Mutant versions of FBW7 from T-ALL patients don't bind NICD.

T cell acute lymphoblastic leukemia (T-ALL) is triggered by mutations in the NOTCH1 receptor. Once activated, this transmembrane protein is clipped to release its intracellular domain (NICD), which then activates the transcription of genes that goad stem cells to become T cells.

Many transformed T cells have too much NICD, as their NOTCH1 proteins carry mutations that protect them from proteasomal degradation. This increase sends many NOTCH1 targets, particularly oncogenes, into overdrive.

The new reports show that NICD levels are also high in some leukemic cell lines that lack NOTCH1 mutations. The defect in these cells lay instead in an enzyme called FBW7, which normally ubiquitinates NOTCH1 so it is recognized by the proteasome. The mutated version, which was also found in some T-ALL patients, failed to bind and ubiquitinate NICD.

Thompson et al. also found that the dysfunctional FBW7 did not bind some of its other targets, such as c-Myc and cyclin-E—oncogenes that are turned on by NICD. This failure might account for the resistance of these cell lines to antileukemia drugs that prevent NOTCH1 receptor cleavage.

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