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Correction

Randolph et al. Vol. 196, No. 4, August 19, 2002. Pages 517–527.

Due to a typographical error, a CD16^- and CD16⁺ were switched in the middle of the third paragraph of page 521. The corrected paragraph appears below.

Differentiation and Migration Analysis after Depletion of CD16⁺ Monocytes.
In some depletion experiments, only the M-DC8⁺ subpopulation of CD14⁺CD16⁺ monocytes was removed. Removal of M-DC8⁺ cells only had no marked effect on the yield or phenotype of reverse-transmigrated cells (unpublished data), but when the depletion scheme eliminated all CD16⁺ monocytes (CD16 depleted), 47 ± 22% (P < 0.05; four experiments) to 66 ± 13% (P < 0.005; four experiments) fewer reverse transmigrated cells were recovered from unstimulated and zymosan-stimulated cultures, respectively (Fig. 6 A). These results are in agreement with the CFSE experiments in Fig. 3. Even after thorough depletion of CD16⁺ monocytes, many reverse-transmigrated cells recovered from cultures receiving only the CD16^- monocytes expressed CD16 upon reverse transmigration, in contrast to the subendothelial monocyte-derived cells from the same cultures (Fig. 6 B). Thus, these cells appear to upregulate CD16 expression during reverse transmigration. When flow cytometry was conducted to analyze the maturation status of cells in the reverse-transmigrated fraction, the number of HLA-DR⁺CD86⁺ cells was 63 ± 14% (average of three experiments; P < 0.005) decreased per unit area of endothelial cell surface when blood CD16⁺ monocytes were depleted from the starting population (Fig. 6 C; cells shown in each group were recovered from an equivalent area of endothelial surface). Moreover, the residual DCs recovered after depletion of CD16⁺ monocytes expressed an order of magnitude less CD86 on the cell surface, indicating that these reverse-transmigrated, CD16^- monocyte-derived cells were less mature than the reverse-transmigrated cells that develop in cultures that contained CD16⁺ blood monocytes.

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This Article Full Text (PDF, 20K) Alert me when this article is cited Services Email this article Similar articles in this journal Alert me to new content in the JEM Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Search for Related Content Social Bookmarking                            What's this?