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Index of Online Supplemental Material for
J. Exp. Med. 10.1084/jem.20080559
Carey et al.

• Figure S1 ;TdT deficiency restricts H-CDR3 length.

• Figure S2 BAFF overexpression does not alter the levels of N⁻ splenic B cells.

• Figure S3 V_H and D_H gene usage in MZ and FO B compartments.

• Figure S4 CDR3 amino acid usage and hydrophobicity in MZ and FO B populations.

• Figure S5 Somatic hypermutation levels in C57BL/6 MZ and FO B cells.

• Figure S6 Representative flow cytometry gates used to identify bone marrow B cell subsets in TdT^+/+/TdT^−/− chimeric mice.

• Figure S7 Representative flow cytometry gates used to identify immature and transitional B cell subsets in the bone marrow.

• Figure S8 Representative flow cytometry gates used to characterize Ig light chain usage in BM and splenic subsets in chimeric mice.

• Figure S9 Representative flow cytometry gates used to identify splenic B cell subsets in TdT^+/+/TdT^−/− chimeric mice.

• Figure S10 Representative flow cytometry gates used to subdivide the FO compartment.

• Figure S11 Adult C57BL/6 TdT^−/− mice have smaller FO compartments than TdT^+/+ mice. n = 2–9 mice per time point.

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