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Index of Online Supplemental Material for
J. Exp. Med. 10.1084/jem.20072102
Liu et al. • Figure S1 Methylation-specific PCR confirmed cyclin D1 promoter DNA hypomethylation in MCL cell lines Granta (G) and NCEB (N), and methylation in mutant cell lines G4 and N1.
• Figure S2 Bisulfite sequencing analysis of a 1:1 mix of genomic DNA and nonhomologous recombinant cell lines.
• Figure S3 FISH analysis of a variant MCL patient, demonstrating loss of one cyclin D1 allele in the neoplastic lymphocytes.
• Figure S4 Western blot analysis of protein levels of cyclin D1 and transcription factors in cell lines.
• Figure S5 Immunofluorescent staining of an MCL patient sample.
• Figure S6 TUNEL assays of G and N parental cell lines, and the G4 and N1 mutant cells lacking the translocated chromosome after infection with retroviruses containing the NPM shRNA or a control shRNA.
• Supplemental text and Table S1 (PDF)
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