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J. Exp. Med. 10.1084/jem.20070447
Scheeren et al.

• Figure S1 Four representative IgD/CD27 dot plots of the fetal tissue analyzed.

• Figure S2 Gestational age of the analyzed fetal tissue and the percentage of IgD⁺CD27⁺ B cells within the IgD⁺ B cells population are shown.

• Figure S3 CD19⁺ sIgD⁻CD27⁺ cells (thick line) expressed CD34 and are BCR⁻, as there was no expression of Ig light chains κ or λ.

• Figure S4 Semiquantitative RT-PCR for AID mRNA expression with RNA from CD19⁺IgD⁺ sorted B cells from the fetal liver fetal BM and fetal spleen (n = 2).

• Figure S5 The reconstitution efficiency is shown around 8 wk after transplantation of human HSCs

• Figure S6 Quantitative real-time RT-PCR for NOTCH2 in RAJI cells transduced with a lentiviral vector expressing a NOTCH2 sh-RNA or a control lentiviral vector.

 

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