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Index of Online Supplemental Material for
J. Exp. Med. 10.1084/jem.20061318
Ling et al. • Figure S1 Scheme for the CPS-priming GFP-PTG challenge experimental system.
• Figure S2 Determination of Toxoplasma gondii viability after ex vivo infection by plaque assay.
• Figure S3 PECs from primed Sjl (CD45.1+) and IGTP-/- (CD45.2+) were mixed together before GFP-PTG infection (MOI of 1, 15 min) ex vivo.
• Figure S4 Rapid parasite elimination does not require B cells and Ig.
• Figure S5 Confocal photomicrographs of dextran-labeled macrophages infected with GFP-PTG
• Figure S6 Parasite degeneration in primed WT macrophages.
• Figure S7 EM photomicrograph showing PVM indentation in a primed WT macrophage.
• Figure S8 Detached parasite plasma membrane (arrow heads) forming complex stacks.
• Figure S9 A partially denuded parasite being enveloped by an autophagosome.
• Figure S10 Electron micrograph of T. gondii remnants
• Video 1 Stacked images of primed and GFP-PTG-infected WT cells stained with LAMP-1
• Video 2 Stacked images of primed and IGTP-deficient cells stained with LAMP-1
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