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Index of Online Supplemental Material for
J. Exp. Med. 10.1084/jem.200500111
Kawakami et al.

• Figure S1 The apoptosis rate of CNS antigen-specific and non-CNS antigen-specific T cells within the CNS.

• Figure S2 The presence of antigen reduces the velocity of non-CNS antigen-specific effector T cells within the CNS.

• Figure S3 The distribution of TCR and LFA-1 on T cells in antibody-stained CNS tissue.

• Table S1 T cell motility analyzed by two-photon imaging versus video microscopy.

• Table S2 The location of stationary and motile T_MBP-GFP cells in different CNS regions.

• Video 1 (2.0 MB) A two-photon video (1 h, 3-min time intervals) of autoreactive effector T_MBP-GFP cells in early EAE spinal cord lesions

• Video 2 (132 k) A magnification of Video 1 depicting a "motile" T_MBP-GFP cell that moves through the CNS tissue rapidly.

• Video 3 (84 K) A magnification of Video 1 depicting a "stationary" T_MBP-GFP cell spinning around a fixed point.

• Video 4 (2.5 MB) "Amoeboid" motility of T_MBP-GFP cells meandering through the CNS tissue

• Video 5 (744 k) A two-photon video of nonencephalitogenic T_OVA-GFP cells within spinal cord lesion 4 d after cotransfer with nonlabeled TMBP cells

• Video 6 (296 k) Polarization of TCR (red) and LFA-1 (blue) towards the fixed point of a stationary T_MBP-GFP cell

• Video 7 (684 k) A stationary T_MBP-GFP cell exhibiting TCR polarization

 

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