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Original Article |
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T Cells in Antierythrocyte Immunoglobulin Transgenic Mice
honjo{at}mfour.med.kyoto-u.ac.jp
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Abstract |
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Tg mice specific for a self-thymus leukemia (TL) antigen in the recombination activating gene (RAG)2–/– background. In the presence of the self-antigen, Tg / T cells increased in number and manifested activated phenotypes. Peritoneal B-1 cells in these mice migrated into mesenteric lymph nodes and differentiated into autoantibody-secreting cells, resulting in strong autoimmune hemolytic anemia. Furthermore, transfer of RAG2–/– x HL bone marrow or peritoneal cells into the peritoneal cavity of RAG2–/– x TCR-/ Tg mice gave rise to donor-derived B-1 cells in mesenteric lymph nodes, and these cells produced the autoantibody. Thus, this study demonstrates that the migration of B-1 cells and differentiation into the antibody-secreting cells can be induced by noncognate T cell help and implies the possibility that / T cells may induce B-1 cell differentiation in vivo.
Key Words: RAG2 deficient • / TCR transgenic • germinal center • peritoneal cavity • hemolytic anemia
We have generated transgenic (Tg) mice with H and L chains of anti-RBC antibody (HL mice [9]). As autoreactive B cells are eliminated at the immature stages in the bone marrow (BM), the number of self-reactive B cells is markedly decreased in the BM, peripheral blood, spleen, and LNs in HL mice. In contrast, the PerC of the HL mice contains a normal number of autoreactive B-1 cells. Therefore, HL mice are one of the ideal models to analyze the mechanisms of B-1 cell activation in vivo. Our previous study using HL mice has shown that peritoneal B-1 cells can be induced to produce autoantibody by T cell–independent stimulation like LPS, suggesting that bacterial infections may be responsible for B-1 cell activation 10. Indeed, over half of the HL mice bred under conventional conditions manifest severe anemia, whereas none of the HL mice bred under specific pathogen-free (SPF) conditions produce the autoantibody despite the full expansion of B-1 cells in the PerC 11. In addition, administration of some Th2 cytokines like IL-5 and IL-10 but not IL-4 can induce differentiation of autoreactive B-1 cells in HL mice 12. These studies indicate that B-1 cells in the PerC can be activated by the noncognate help of T cells, probably by the secretion of cytokines.
More recently, we have suggested that the migration of B-1 cells out of the PerC may be essential for activation and differentiation of B-1 cells 13. The alymphoplasia (aly) mutation of the nuclear factor (NF)-
To test the hypothesis that migration and differentiation of B-1 cells may be regulated by noncognate T cell help, we have developed a mouse line by crossing the HL mice with TCR-
Preparation of Single Cell Suspensions.
Detection of Anti-RBC Autoantibody Production.
Enzyme-linked Immunospot Assay.
Flow Cytometry.
Cytoplasmic Staining.
Adoptive Transfer.
Immunohistological Analysis.
ELISA.
Introduction
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Introduction
Materials and Methods
Results
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B cells are divided into two subsets, B-1 and B-2, by their distinct tissue distribution, antigen specificity, and surface markers. B-1 cells are a predominant population in the peritoneal cavity (PerC), whereas they are rare in spleen and extremely few, if any, are in LNs 12. B-1 cells often recognize self-antigens with low affinity and broad specificities 34. In contrast, B-2 cells recognize a wide variety of antigens with high affinity and strong specificities. B-1 cells have higher surface IgM levels than B-2 cells 5. B-1 cells are also distinguished from B-2 cells by their unique surface markers such as CD5, Mac-1, and low B220 expression 6. As B-1 cells are often involved in the production of pathogenic and natural autoantibodies 3789, their generation, migration, and differentiation into antibody-producing cells are important issues in understanding autoimmune diseases and innate immunity. 
B–inducing kinase gene 14 causes the defect of the migration of peritoneal B-1 cells into the gut-associated lymphoid tissue, especially lamina propria (LP). This migration defect is associated with the defect of IgA production in the LP. Indeed, it has been suggested that there is a link between peritoneal B cells and IgA plasma cells in the LP 15. Several lines of evidence demonstrated that half of the IgA plasma cells in the LP are derived from PerC B-1 cells. 161718. / Tg mice on the recombination activating gene (RAG)2–/– background, in which all T cells recognize a self-thymus leukemia (TL) antigen. In these mice, the / T cells became activated in the presence of the self-antigen. In addition, B-1 cells migrated into mesenteric LNs (MLNs) and differentiated into autoantibody-producing cells, resulting in severe autoimmune hemolytic anemia in the HL mice. Thus, this study demonstrates that the migration of B-1 cells and differentiation into antibody-producing cells can be induced by a T cell help that is not through so-called cognate interaction, and implies the possibility that / T cells may induce B-1 cell differentiation in vivo. In addition, these results clearly support the hypothesis that migration from the PerC is required for the terminal differentiation of B-1 cells.
Materials and Methods
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Introduction
Materials and Methods
Results
Discussion
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Tg Mice.
Tg mouse lines carrying either the H or L chain gene for the anti-RBC antibody (4C8 antibody) were established and double-Tg (HL) mice with H and L chain transgenes were generated as described previously 9. RAG2-deficient mice 19 and Tg mice carrying productively rearranged V2/V5 TCR genes that recognize a TL antigen of H-2b haplotype (KN6 Tg mice [20]) were crossed to obtain RAG2-deficient KN6 Tg heterozygous mice. By mating these mice with H and L chain Tg mice, we finally obtained RAG2-deficient KN6 Tg x HL mice. The mice were maintained under SPF conditions in our animal facility. The H-2 haplotype was determined by flow cytometry with peripheral blood cells. In this study, mice were analyzed at 8–14 wk of age.
LP lymphocytes and intraepithelial lymphocytes (IELs) of the small intestine were isolated by the method described previously 2122. BM cells were prepared by flashing femur bones with a staining buffer (PBS containing 2% FCS and 0.05% sodium azide). Peritoneal washout cells were prepared by flushing the PerC with 10 ml of the staining buffer.
The amounts of autoantibodies on erythrocytes were measured by flow cytometry with FITC-goat anti–mouse IgM antibody (ICN Biomedicals/Cappel) as described previously 9. Mean fluorescence intensity was measured by FACSCaliburTM (Becton Dickinson).
The number of antibody-producing cells in HL mice was determined by enzyme-linked immunospot (ELISPOT) assay with goat anti–mouse IgM antibody (Cappel) as described previously with slight modification 23. We used alkaline phosphatase–goat anti–mouse IgM antibody (Southern Biotechnology Associates, Inc.).
The following monoclonal antibodies were used: FITC-conjugated antibodies anti–TCR-
/β (H57-597) and anti-V2 TCR (UC3-10A6); PE-conjugated antibodies anti-CD45R/B220 (RA3-6B2), anti-CD11b (Mac-1 chain, M1/70), anti–TCR-/ (GL3), anti-CD5 (53-7.3), anti-CD43 (S7), anti-CD44 (IM7), and anti-CD69 (H1.2F3); and Cy-Chrome–conjugated antibody anti-CD45R/B220. These antibodies were purchased from BD PharMingen. FITC-F(ab')2 fragment of goat anti–mouse IgM was from Cappel. PE-streptavidin was from Dako. Antiidiotype antibody (S54) against the transgene-encoded anti-RBC antibody (4C8) was biotinylated according to the directions provided by the manufacturer (Pierce Chemical Co.). Flow cytometric analysis was performed as described 24 by a FACSCaliburTM with CELLQuestTM software v3.1 (Becton Dickinson). After excluding dead cells by propidium iodide gating, cells present in the lymphocyte gate defined by forward and side light scatters were analyzed.
Single cell suspensions from MLNs, PerC, and LP of small intestine were mounted on glass slides by cytocentrifuge preparation (Cytospin 3; Shandon Inc.). They were fixed in methanol for 15 min, and blocked with 5% heat-inactivated normal goat serum. Slides were incubated with FITC-F(ab')2 fragment of goat anti–mouse IgM (Cappel) for 30 min at room temperature. After the final wash, the slides were mounted and examined under a confocal fluorescence microscope.
A total of 1.5 x 107 BM cells or 7 x 106 PerC cells from RAG2-deficient HL mice were injected intraperitoneally into RAG2–/– recipient mice with or without KN6 Tg. After 6 wk, the mice were killed, and lymphocytes in various lymphoid organs were analyzed by flow cytometry and cytoplasmic staining. Autoantibody levels on erythrocytes were measured by flow cytometry.
Sections of MLN were fixed in formalin and stained with hematoxylin and eosin by standard methods. Fluorescence immunohistology was performed on frozen sections. Sections of 6 µm were cut from tissue blocks and mounted onto glass slides. The sections were air dried for 30 min, fixed in 4% paraformaldehyde for 15 min, and blocked with 5% heat-inactivated normal goat serum. The sections were stained with biotin–anti-mouse IgM antibody (Cappel), followed by FITC–anti–peanut agglutinin (PNA; Vector Laboratories) or anti-V2 TCR (BD PharMingen) antibody and Texas red–streptavidin (Life Technologies). After the final wash, the slides were mounted and examined under a confocal fluorescence microscope.
Amounts of IL-4, IL-5, IL-6, and IL-10 in the serum from mice were assessed using the mouse IL-4, IL-5, IL-6, and IL-10 ELISA systems (Amersham Pharmacia Biotech) according to the manufacturer's protocol.
Results
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Introduction
Materials and Methods
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Activated Tg / T Cells Induce Autoantibody Production and Anemia in HL x RAG2–/– Mice.
RAG2–/– x KN6 Tg mice have neither conventional T cells nor B cells, but have Tg / T cells that recognize an autologous TL antigen encoded by one of the MHC class I genes designated 27b 25. The MHC class I gene 27b is mapped on the MHC TL region in C57BL/6 strain (H-2b) but not in BALB/c (H-2d). The TL antigen is mainly expressed on thymocytes, splenic T and B cells, PerC Mac-1+, and CD5+ cells. Although Tg / T cells are mostly deleted in RAG2+/– mice with the H-2b haplotype as described previously 20, higher numbers of Tg / T cells were identified in RAG2–/– mice (Fig. 1 A), probably because they escaped clonal deletion more easily in the absence of endogenous lymphocytes expressing the TL antigen. The percentages of the Tg / T cells increased dramatically in MLNs and PerC in RAG2–/– mice with the H-2b haplotype. However, the total cell number in PerC (0.8 ± 0.2 x 105) of these mice was somewhat decreased compared with that of RAG2+/– mice (1.5 ± 0.5 x 105), probably because the continuous interaction with Mac-1+ cells, which express relatively large amounts of the TL antigen 25, resulted in rapid apoptosis of activated Tg / T cells. In contrast, the total cell number in MLNs (23.8 ± 6.1 x 105) was increased in RAG2–/– compared with that in RAG2+/– mice (5.6 ± 1.1 x 105). Thus, the size of the Tg / T cell compartment in MLNs is clearly enlarged in RAG2–/– mice with the H-2b haplotype.
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/ T cells in RAG2–/– x KN6 Tg x HL mice with the H-2b haplotype, we analyzed cells in spleen, MLNs, PerC, and IELs by flow cytometry with anti-V2 antibody and either anti–TCR-/, anti-CD44, or anti-CD69 antibody. Tissue distribution of / T cells in these mice was indistinguishable from that of RAG2–/– x TCR-/ Tg mice with the H-2b haplotype (data not shown). As shown in Fig. 1 B, Tg / T cells in the H-2b haplotype mice downregulated TCR expression slightly, whereas their cell sizes were enlarged as assessed by forward scatter, and their expression levels of CD44 and CD69, activation markers of peripheral T cells 262728, were enhanced compared with Tg / T cells in the H-2d haplotype mice. These results indicate that the majority of Tg / T cells in the MLNs and PerC are activated in RAG2–/– x KN6 Tg x HL mice with the H-2b haplotype.
To assess whether the presence of activated Tg / T cells enhances autoantibody production and anemia in HL mice, we examined the amount of the autoantibody bound to erythrocytes and the incidence of anemia in the presence or absence of the H-2b haplotype. We identified large amounts of the autoantibody bound to erythrocytes in RAG2–/– x KN6 Tg x HL mice with the H-2b haplotype, whereas we could not detect significant amounts of the autoantibody in other control mice that do not have activated Tg / T cells in MLNs (Fig. 2 A). Similarly, induction of anemia was observed only in RAG2–/– x KN6 Tg x HL mice with the H-2b haplotype (Fig. 2 B). These results indicate that the presence of activated / T cells is critical for induction of the autoantibody production and anemic phenotype in HL mice.
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/ T Cells and Autoantibody-producing B Cells in the MLNs of HL Mice./ T cells and differentiate into autoantibody-producing cells in MLNs, we performed immunohistological analysis. MLNs of C57BL/6 mice have primary and secondary follicles which contain PNA+ germinal centers, and plasma cells are found in the paracortex of LNs (Fig. 5, top panels). In contrast, PNA+ germinal centers were not detected in follicles of MLNs of RAG2–/– x KN6 Tg x HL mice with the H-2b haplotype, although we could identify small follicles. In these mice, Tg / T cells were accumulated in the paracortex area of B cell follicles, whereas IgM+ plasma cells were found near Tg / T cells (Fig. 5, bottom right panel). MLNs of RAG2–/– x KN6 Tg x HL mice without the H-2b haplotype did not contain IgM+ cells (data not shown). These results indicate that activated Tg / T cells helped activation and terminal differentiation of autoreactive B-1 cells in vivo without germinal center formation.
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/ T cells, we transferred BM cells from RAG2–/– x HL mice into the PerC of RAG2–/– x KN6 Tg mice with or without the H-2b haplotype and analyzed lymphoid tissues 6 wk after the transfer. Flow cytometric analyses of PerC cells revealed that a large number of IgM+ cells could be detected in recipient RAG2–/– x KN6 Tg mice regardless of the H-2 haplotype (Fig. 7). In addition, the majority of PerC IgM+ cells expressed the Mac-1 antigen but not CD5 (data not shown), suggesting that they belong to the B-1b cell subset 30. In contrast, MLNs of RAG2–/– x KN6 Tg mice with the H-2b haplotype but not those without the H-2b haplotype contained IgM+ cells, the majority of which also express Mac-1. It is worth noting that MLNs also contained a large number of Mac-1 strongly positive macrophages when activated Tg / T cells were present. Moreover, we identified a significant amount of the autoantibody bound to erythrocytes only in RAG2–/– x KN6 Tg mice with the H-2b haplotype. Spleen and BM of recipient mice contained few IgM+ B cells. Taken together, these results suggest that BM cells from RAG2–/– x HL mice can generate B-1b cells in PerC and that activated / T cells can induce these B-1b cells to migrate to MLNs and to differentiate into autoantibody-producing cells.
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/ T cells can induce these B-1 cells to migrate to MLNs and to differentiate into autoantibody-producing cells.
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/ T cells are either activated or tolerized by the TL antigen associated with the H-2b locus. However, in the RAG2 deficiency, a large number of activated Tg / T cells escape tolerance and expand in MLNs (Fig. 1). In this system, we have shown that the anti-RBC antibody level in the serum was markedly elevated, resulting in reduction of the hematocrit value (Fig. 2). In addition, the numbers of PerC B-1 cells were drastically reduced with concomitant increase of IgM+ cells in MLNs (Fig. 4). IgM+ cells in MLNs are actually antibody-secreting cells (Fig. 2 C) and show plasma cell morphology (Fig. 3). These antibody-producing cells were found in MLNs without forming germinal centers (Fig. 5). Serum IL-10 level was elevated in these mice (Fig. 6). These results suggest a scenario in which Tg PerC B-1 cells migrate to MLNs and differentiate into antibody-producing plasma cells in the presence of activated / T cells even with different antigen specificity. This hypothesis gained further support by the lymphocyte transfer experiment of RAG2–/– x HL BM or PerC cells into the PerC of RAG2–/– x KN6 Tg mice. Only when Tg / T cells are activated by the presence of the H-2b haplotype do donor-derived B-1 cells migrate to MLNs and produce the autoantibody (Fig. 7 and Fig. 8).
The results indicating that Tg B-1 cells can be activated and induced to migrate to MLNs by a T cell help that is not through so-called cognate interaction agree with our previous reports that LPS, IL-5, or IL-10 administration can activate Tg B-1 cells in the PerC, giving rise to the autoantibody production and autoimmune hemolytic anemia in HL mice 1012. As LPS cannot directly stimulate T cells, macrophages are likely to be another source of cytokines and chemokines for migration and activation of PerC B-1 cells as proposed previously 29. In this study, we have also shown that activated / T cells not only themselves accumulate in MLNs but also induce accumulation of macrophages in MLNs (Fig. 7), suggesting that activated T cells may secrete some chemokines to induce migration of macrophages 313233. Macrophages, in turn, may communicate with activated T cells by cytokines 3435. As B cells express TL antigen, there is a possibility that / T cells directly interact with Tg B-1 cells and can give "semi-cognate" help. However, we could not detect typical germinal centers in MLNs (Fig. 5), indicating that Tg B-1 cells did not differentiate into plasma cells by cognate help of T cells. In addition, the serum IL-10 levels were significantly increased in these mice. Taken together, these results suggest that both activated T cells and macrophages may be involved in activation, migration, and differentiation of peritoneal B-1 cells in a noncognate manner.
/ T cells have unique features in contrast to /β T cells 3637. / T cells develop earlier in ontogeny than /β T cells. / T cells expressing specific V chain show unique tissue distribution such as in the epidermal and mucosal epithelia in the adult mouse. Some / T cells develop mainly from fetal progenitors, a common feature with B-1 cells. / T cells recognize nonpeptide antigens like pyrophosphate derivatives, as well as polypeptide antigens like nonclassical class I molecules and heat shock proteins 38394041. Thus, it has been suggested that / T cells are involved in the primary and innate host defense mechanisms. As B-1 cells are also involved in innate immunity 542, / T cells may cooperate with B-1 cells in such an evolutionally ancient host defense system.
Recently, we showed that normal peritoneal B-1 cells migrate to MLNs and LP to differentiate into antibody-producing cells. The alymphoplasia mutation in NF-B–inducing kinase gene causes severe defects in migration and differentiation of peritoneal B-1 cells 13. This suggests that PerC B-1 cells respond to chemokine for their migration and that NF-B is involved in the signal transduction process of chemokine receptors. Unlike the findings of previous reports that antibody-secreting cells can be found in PerC 10122329, our recent study clearly indicates that peritoneal B-1 cells have to migrate to either LNs or LP to differentiate 13. B-1 cell differentiation into plasma cells depends on chemokines that stimulate migration of PerC B-1 cells into LP of the gut or MLNs where B-1 cells become plasma cells probably by stimulation with cytokines. LP of the gut and MLNs are preferred targets of migration probably because of the anatomical localization. This study confirms and extends the latter finding. Interestingly, B-1 cells in RAG2–/– x KN6 Tg x HL mice carrying the H-2b haplotype predominantly migrate into MLNs but not into the LP. MLN is also the preferred site of HL B-1 cell migration under conventional conditions 9. HL Tg B cells might be deleted during migration to LP of the gut because they have to go into blood circulation.
We have identified B220–IgM+ cells as the major population of IgM+ cells in the MLNs of RAG2–/– x KN6 Tg x HL mice in the presence of the H-2b haplotype. As the majority of IgM+ cells in the MLNs in these mice show plasma cell morphology (Fig. 3) and a significant fraction (10–1) secretes IgM (Fig. 2 C and 4 A), we assume that B220–IgM+ cells are most likely plasma cells because the presence of B220–IgA+ plasma cells in LP of the gut in various mouse strains 21. As almost all B-2 cells are deleted in the periphery and only PerC B-1 cells survived in this system, these IgM+B220– plasma cells are most likely derived from PerC B-1 cells. In general agreement with this conclusion, when BM and PerC cells were transferred into the PerC of RAG2–/– x KN6 Tg mice, we found B-1 cells in PerC and plasma cells in MLNs (Fig. 7 and Fig. 8).
In summary, we have shown that autoreactive B-1 cells in PerC migrate into MLNs and differentiate into antibody-producing cells by a T cell help that is not through so-called cognate interaction in HL Tg mice. This help involves other type of cells, probably macrophages, for providing various chemokines and cytokines. This result agrees with and explains our previous findings that HL Tg PerC B-1 cells can be activated by cytokines (IL-5 and IL-10) or LPS, and that normal PerC B-1 cells have to migrate to MLNs or LP of the gut in order to differentiate into antibody-producing cells.
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Acknowledgments |
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/ Tg mouse, Ms. T. Taniuchi and M. Tanaka for their excellent technical assistance, and Ms. K. Saito for manuscript preparation. This work was supported by grants for the Center for Excellence Program from the Ministry of Education, Science, Sports and Culture of Japan.
Submitted: 5 July 2000
Revised: 28 September 2000
Accepted: 9 October 2000
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References |
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| TABLE OF CONTENTS |
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) and without the H-2b haplotype (
) were assessed by ELISA. Horizontal long bars indicate the minimum detectable levels of each cytokine. The Student's t test for unpaired data was used to compare the values between two groups.
