|
|
|
|
|
|
|
||
Original Article |
bjorkman{at}cco.caltech.edu
 |
Abstract |
|---|
 Top Abstract IntroductionMaterials and MethodsResultsDiscussionReferences |
|---|
Key Words: β-trefoil protein • hydrogen bond network • multilectin receptor • pituitary hormones • sulfated GalNAc
MR is a 180-kD type I transmembrane protein that contains three extracellular regions: an NH2-terminal cysteine-rich domain (Cys-MR), a domain containing fibronectin type II repeats, and eight tandem C-type lectin carbohydrate-recognition domains (CRDs; 4). The extracellular domains are linked to a transmembrane region and a small cytoplasmic domain. These structural characteristics are shared among members of the MR family, which includes the phospholipase A2 receptor 7 8 9, the dendritic cell receptor DEC-205 10, and an MR-like receptor expressed in epithelium 11. The CRDs of the extracellular region mediate calcium-dependent binding to sugars such as mannose, fucose, and N-acetylglucosamine that are commonly found on microorganisms, but rarely seen in sufficient density in terminal positions of mammalian oligosaccharides 12 13. Three-dimensional structures of members of the C-type lectin superfamily 14 have facilitated mechanistic studies of sugar binding by the MR CRDs 15, and the crystal structure of the principal mannose-recognition domain of MR (CRD4) has now been determined.
Recent studies demonstrate that the Cys-MR region also has carbohydrate-binding properties. Cys-MR binds to glycoproteins containing sulfated sugars that terminate in SO4-4GalNAcβ1,4GlcNAcβ1-, such as the sulfated hormones lutropin and thyroid stimulating hormone 16 17 18. Further studies of its carbohydrate-recognition properties indicate that Cys-MR can also bind chondroitin sulfates A and B and sulfated oligosaccharides of the Lewisa and Lewisx types 19. MR binding of hormones is thought to regulate their bioavailability 20 and facilitate rapid clearance of lutropin from the serum through binding to MR on hepatic endothelial cells 21. Studies using an Fc chimeric protein containing mouse Cys-MR revealed the presence of additional ligand(s) for Cys-MR. The fusion protein binds to cells in germinal centers and to macrophages from the splenic marginal zone and lymph node subcapsular sinus 22. The Cys-MR interaction with cells in the spleen results from the binding of sulfated carbohydrates 19. These results suggest that Cys-MR directs MR-bearing cells toward germinal centers during immune responses, and that sulfated carbohydrate ligands regulate the trafficking and function of cells bearing MR.
To establish the mechanism of sulfated sugar recognition by Cys-MR, we solved crystal structures of Cys-MR alone and complexed with the monosaccharide 4-sulfated-N-acetylgalactosamine (4-SO4-GalNAc). The structures of Cys-MR and the principal mannose-binding domain of MR (CRD4) provide a framework for modeling the structures of domains in the MR family of proteins, and facilitate an understanding of the role of MR in binding carbohydrates in antimicrobial immunity and in hormone regulation.
Crystallization and Data Collection.
Introduction
Top
Abstract
Introduction
Materials and Methods
Results
Discussion
References
The innate immune response is critical for detection and elimination of infectious microorganisms. Carbohydrates on microbial cell walls are common targets for pattern recognition receptors on immune cells such as macrophages, which recognize a variety of antigens 1. The mannose receptor (MR), expressed on some macrophages, epithelial, and endothial cells 2 3, is the prototype member of a family of multilectin receptors that recognize carbohydrates on the cell walls of infectious organisms 4. Carbohydrate recognition by MR facilitates macrophage uptake of bacteria, yeast, and parasites, thereby contributing to innate immunity towards these pathogens. MR is also thought to play a role in the adaptive immune response by transporting antigens to MHC class II–containing compartments in immature dendritic cells for antigen processing and presentation to T cells 5, and by delivering mycobacterially derived glycolipids to endosomes for T cell presentation by the MHC class I–like molecule CD1b 6.
Materials and Methods
Top
Abstract
Introduction
Materials and Methods
Results
Discussion
References
Expression and Purification of Soluble Cys-MR.
A Cys-MR-Fc fusion protein (hydrophobic signal sequence and the first 139 residues of mouse MR, a 2-residue linker, and the Fc region of human IgG1) was produced in 293T cells 22. Supernatants from transfected cells were harvested 13 d after transfection. The Cys-MR-Fc protein was collected on protein A beads (Pierce Chemical Co.), eluted with elution buffer (Pierce Chemical Co.), dialyzed into PBS, and concentrated to 5 mg/ml using a centricon filter (Amicon). The chimeric protein was digested with papain coupled to agarose beads (0.4 U/mg of Cys-MR-Fc; Sigma Chemical Co.) in PBS, 10 mM EDTA, 10 mM L-cysteine at 37°C for 1 h. The reaction was terminated by adding iodoacetamide to a concentration of 30 mM and centrifuging out the papain beads. Fc fragments and undigested Cys-MR-Fc were removed using protein A beads. The sequence derived from NH2-terminal sequencing of purified Cys-MR begins 19 residues after the initiating methionine (NH2-terminal sequence of mature protein is Leu-Asp-Ala-Arg-Gln; data not shown). Purified human MR starts 18 residues after the initiating methionine with the sequence Leu-Leu-Asp-Ala-Arg-Gln 23, indicating that the position at which the hydrophobic leader peptide is cleaved in the human and mouse versions of MR differs by one residue. The numbering scheme used in this paper corresponds to the numbering of mature mouse Cys-MR.
Crystals (space group P212121; a = 39.6 Å, b = 41.3 Å, c = 98.8 Å; one molecule per asymmetric unit) were grown at 4°C in 1:1 hanging drops containing Cys-MR (40 mg/ml in 10 mM Hepes, pH 7.4, 10 mM NaCl) and 30% (wt/vol) PEG 8000, 0.2 M (NH4)2SO4. Before data collection, crystals were transferred to a cryoprotectant solution (10 mM Hepes, pH 7.0, 32% PEG 8000, 0.2 M [NH4]2SO4, 10% glycerol). A native data set used for multiple isomorphous replacement including anomalous scattering (MIRAS) phase determination (Native I; Table ) was collected at –150°C from a single crystal to 1.9 Å using an R-AXIS II detector (Molecular Structures Corp.) mounted on a rotating anode generator (model R200; Rigaku). Heavy atom derivative data sets (prepared by soaking native crystals in synthetic mother liquor containing heavy atoms for 7 d) and the 1.7 Å native data set used for refinement (Native II; Table ) were collected using the same procedures. Crystals in complex with 4-SO4-GalNAc (space group P212121; a = 39.4 Å, b = 41.5 Å, c = 100.3 Å; one molecule/asymmetric unit) were obtained by soaking Cys-MR crystals in 2 mM 4-SO4-GalNAc (Sigma Chemical Co.), 32% PEG 8000, 0.2 M (NH4)2SO4 for 10 d at 4°C. Native data were collected to 2.2 Å as described above. Data were processed and scaled with the HKL package ( 24; Table ).
|
in difference Fourier maps. The refined model (Rfree = 20.8%; Rcryst = 19.8%) includes residues 2–135 of Cys-MR, 264 water molecules, 1 sulfate group, and no residues in disallowed regions of the Ramachandran plot. There is no ordered density for residues 1 and residues 136–139 of Cys-MR, or for the two-residue linker inserted before the Fc. Ordered density is not observed in the vicinity of the potential N-linked glycosylation site at Asn87.
The structure of Cys-MR complexed with 4-SO4-GalNAc was solved at 2.2 Å resolution by molecular replacement using native Cys-MR as a search model. The model was refined using CNS 29 as described above. An initial model of 4-SO4-GalNAc was obtained by combining coordinates for GalNAc and a sulfate group, ensuring proper connectivity and bond angles. The ligand was modeled into a large continuous electron density feature with the sulfate group positioned identically to the sulfate in the native structure. The 4-SO4-GalNAc molecule is well defined in Fo-Fc maps contoured at 3.0 and 2Fo-Fc maps contoured at 1.1 , with the exception of the N-acetyl group, which has weak electron density. The refined model (Rfree = 23.2%; Rcryst = 21.3%) includes 134 amino acids (residues 2–135 of Cys-MR), 153 water molecules, and the 4-SO4-GalNAc group. None of the residues are in disallowed regions of the Ramachandran plot.
Fig. 1A and Fig. B, Fig. 2A–D, and Fig. 3A and Fig. B, were made using MOLSCRIPT 31 and Raster3D 32. Electrostatic potentials were calculated, and Fig. 2 E was made using GRASP 33.
|
|
|
|
Results |
|---|
|
TopAbstractIntroductionMaterials and MethodsResultsDiscussionReferences |
|---|
and β 38 39, and the actin binding protein hisactophilin 40. Members of this family generally share little or no detectable sequence similarity ( Fig. 1 C), but weak sequence similarity between Cys-MR and the ricin B chain was previously reported 41, thus anticipating the structural similarity. Following the conventions for the description of the fold 35 34, Cys-MR contains three lobes (I, β strands 1–4; II, β strands 5–8; and III, β strands 9–12; Fig. 1A and Fig. B) that are related by approximate three-fold internal symmetry.
The six cysteines for which Cys-MR is named are involved in three disulfide bonds ( Fig. 1A–C). The first, between Cys16 and Cys30, connects β strand 2 and the loop between strands 3 and 4 in lobe I. The second disulfide bond, located in lobe II, is related to the first by the pseudo three-fold symmetry, connecting Cys55 in strand 6 to Cys72 in the loop between strands 7 and 8 ( Fig. 1 B). Two disulfide bonds are found in similar positions in the structure of the ricin B chain ( Fig. 1A and Fig. C), which allowed correct prediction of the observed disulfide bonding pattern of Cys-MR 41. The third disulfide bond in Cys-MR is not related to the others by the internal three-fold symmetry; instead, it connects Cys83 in strand 8 to the Cys130 in the extended polypeptide chain COOH-terminal to strand 12. This disulfide bond anchors the extended region after strand 12, a feature not found in β-trefoil proteins such as ricin and FGF ( Fig. 1 C), to the remainder of the Cys-MR structure. In lobe III, the residues at the positions analogous to the cysteines in lobes I and II are Tyr97 in strand 10 and Gly113 in the loop between strands 11 and 12, whose C atoms are separated by 7.1 Å (compared with 5.0 and 5.1 Å for the C separation between the cysteines involved in the disulfide bonds in the first two lobes). Even if residues 97 and 113 were substituted by cysteines, a disulfide bond could not form because the C atom of residue 113 faces in the opposite direction to the side chain of residue 97 ( Fig. 1 B). The reorientation of residue 113 compared with its lobe I and II counterparts occurs because the loop between strands 11 and 12 is shorter than the counterpart loops in lobes I and II ( Fig. 1B and Fig. C). Lengthening the loop between strands 11 and 12 would likely disturb ligand binding by Cys-MR, as this loop forms the side of the ligand binding pocket (see below).
The Cys-MR structure includes an as yet unidentified ligand that cocrystalizes with the protein. Electron density that cannot be accounted for by the amino acid sequence, N-linked carbohydrate, or water molecules is found in lobe III near the loop connecting strands 11 and 12 ( Fig. 2 A, and Table ). Components of the crystallization buffer, which includes ammonium sulfate and Hepes (N-[2-hydroxyethyl] piperazine-N'-[2-ethanesulfonic acid]; see Materials and Methods), are possible sources of the ligand. A sulfate group fits into the tetrahedral portion of the electron density, and an anomalous difference Fourier map calculated using the native data and refined phases revealed a 7.0 peak at the presumed sulfur atom location ( Fig. 2 A). The unidentified ligand is unlikely to be ammonium sulfate since the remaining portion of the density is too large to represent an ammonium group and ammonium sulfate is expected to be completely ionized under the near-neutral pH conditions of the crystallization. A molecule of Hepes can be fit into the electron density with its SO3 group occupying the position of the sulfate group, but there is no density to account for half of the piperazine ring or the hydroxyethyl group. In the absence of conclusive identification of the ligand, it was modeled as a sulfate group in the refined native Cys-MR structure (Table ).
|
atoms; 0.54 Å rms deviation for all atoms); thus, there are no major main chain or side chain rearrangements induced by ligand binding. Since the Cys-MR binding site is occupied in the native structure, we cannot address whether conformational changes occur upon ligand binding. However, ligand-induced conformational changes have not been observed in other lectin structures, which, in their unoccupied states, generally contain preformed binding sites in which ordered water molecules contribute hydrogen bonds that substitute for hydrogen bonds from the sugar hydroxyls 42. The 4-SO4-GalNAc ligand binds in a pocket formed on one side by residues 111–116 in the loop between strands 11 and 12, on the other side by the side chains of residues including Asn102 and Trp117, and on the bottom by the Asn99 side chain. The sulfate portion of 4-SO4-GalNAc points into the pocket, making extensive hydrogen bonds and van der Waals contacts with residues in the 11–12 loop region ( Fig. 2 C). The corresponding region of DEC-205 shares little sequence similarity with Cys-MR and contains a deletion ( Fig. 1 C), thus rationalizing the observation that the cysteine-rich domain of DEC-205 does not bind lutropin 19.
The galactose ring of 4-SO4-GalNAc, which is in the chair conformation, is positioned with the polar A face exposed to solvent and the nonpolar B face stacked against Trp117, making numerous van der Waals interactions with Asn102, Leu116, and Trp117 (Table , and Fig. 2 D). The stacking interaction between the galactose ring and Trp117 is reminiscent of interactions seen in the structures of other protein–galactose complexes, including plant lectins, bacterial toxins, galectins, and the galactose-binding mutant of mannose-binding protein, in which the nonpolar face of the galactose moiety stacks against the tryptophan or phenylalanine side chains ( 42; Fig. 2 D). In addition to van der Waals interactions, the 3- and 6-hydroxyl groups of the galactose ring make hydrogen bonds with Asn102 and Leu116, and participate in two water-mediated hydrogen bonds with the protein ( Fig. 2 C, and Table ). The N-acetyl group makes no contacts with the protein.
The specificity of Cys-MR for sulfated carbohydrates is explained at the structural level by the multitude of interactions between the protein and the sulfate group of 4-SO4-GalNAc. Of the eight direct hydrogen bonds between Cys-MR and the ligand, six involve the sulfate group (Table ). The first sulfate oxygen accepts two hydrogen bonds, one from the Asn99 side chain and the other from the Asn102 side chain. The other free oxygens of the sulfate group accept four hydrogen bonds, all involving main chain NH groups of the protein – the NH groups of Ser114 and Gly115 with the sulfate OS2, and the NH groups of Leu116 and Trp117 with the sulfate OS3. The NH groups have no rotational freedom as they are part of the peptide bond, and the residues that they belong to are further constrained by hydrogen bonds involving their peptide CO groups. Thus, the Ser114 CO hydrogen bonds to Arg119 NH2, the Gly115 CO hydrogen bonds to Ser118 NH and Arg119 NE, the Leu116 CO hydrogen bonds to Asn10 ND2, and the Trp117 CO hydrogen bonds to the NH group of Tyr100. As many of the groups on the protein that are hydrogen bond donors to the sulfate oxygens have no rotational freedom and belong to residues that are constrained in the protein, the precise fold of the polypeptide backbone is critical for the geometry of the Cys-MR ligand binding site.
Although the sulfate portion of 4-SO4-GalNAc is negatively charged, the binding site on Cys-MR does not have a positive electrostatic potential ( Fig. 2 E) and the sulfate group does not interact with positively charged residues or counter ions (Table ). These features are shared with another protein that binds sulfate, the sulfate-binding protein (SBP) from Salmonella typhimurium 43. Based on studies of SBP and other proteins that bind charged ligands without using counter ions, a mechanism for stabilizing isolated charges on groups sequestered in proteins was proposed 44. In this mechanism, hydrogen bonds, especially those formed with highly polarized peptide units, stabilize charges on buried groups, which are further dispersed by hydrogen bond arrays. The sulfate binding interactions of both Cys-MR and SBP conform to three common features of proteins that bury isolated negative charges: 1 the interactions are mediated primarily through hydrogen bonds rather than salt bridges or counter ions; 2 most of the hydrogen bonds involve peptide NH groups, and 3 the peptide NH groups associated with the sulfate group are coupled to a hydrogen bond array within the protein.
The Cys-MR ligand binding site differs in many respects from the binding sites of other proteins with β-trefoil folds, including the ricin B chain and FGF, both of which bind carbohydrates. The galactose binding site of the ricin B chain is in lobe I instead of lobe III ( Fig. 1A and Fig. C). However, in common with the interaction between the galactose ring of 4-SO4-GalNAc and Cys-MR, the galactose ligand of ricin B is stacked against a tryptophan residue (Trp37), although, compared with the Cys-MR tryptophan, the opposite face of the ricin tryptophan ring contacts the galactose ( Fig. 2 D). While FGF binds a sulfated carbohydrate using a region of its structure analogous to the Cys-MR ligand binding site (the strand 11–12 region of lobe III; Fig. 1A and Fig. C), the FGF site is a fairly flat, positively charged surface that uses electrostatic interactions for ligand recognition 45, by contrast with the neutral pocket that constitutes the Cys-MR binding site, in which electrostatic interactions do not play a role in ligand binding.
Carbohydrate Binding Specificity of Cys-MR.
The structure of Cys-MR bound to 4-SO4-GalNAc can be used to rationalize the observed binding specificity of Cys-MR for sulfated carbohydrates. Biochemical experiments 19 concluded that Cys-MR binds to GalNAc or galactose when it is sulfated on the C3 or C4 position, but only weakly if the sulfate is at the C6 position 19. The lack of discrimination between sulfated galactose and sulfated N-acetylgalactosamine is explained by the absence of contacts between the N-acetyl group of 4-SO4-GalNAc and Cys-MR (Table ). To understand the mechanism that the position of the sulfate is discriminated, we used models of sulfated GalNAc molecules ( Fig. 3 A) to predict the interactions between Cys-MR and GalNAc that is sulfated at the C3 or C6 positions and compared these with the interaction observed in the Cys-MR/4-SO4-GalNAc complex structure. For each carbohydrate model, we positioned the sulfate group in the Cys-MR binding site in the position observed in the crystal structure of Cys-MR complexed with 4-SO4-GalNAc and allowed rotational freedom about the galactose C6-sulfate oxygen and the galactose C5–C6 bonds. In the case of 3-SO4-GalNAc, the galactose ring can be fit into the binding site without steric hindrance, making extensive van der Waals contacts with Trp117. The galactose ring of 6-SO4-GalNAc also fits into the binding site without steric hindrance, but cannot be rotated into a position in which the sugar ring makes extensive van der Waals contacts with Trp117.
The same binding study concluded that Cys-MR recognizes sulfated GalNAc when it is in a terminal, rather than an internal, position within an oligosaccharide chain 19. For example, adding a terminal uronic acid with a β1-3 linkage to a 4-SO4-GalNAc group prevents binding to Cys-MR. From the mode of binding revealed by the crystal structure, this result is expected since linking a saccharide group to the C3 position of 4-SO4-GalNAc should prevent access of the sulfate group to the binding site ( Fig. 2 C). By contrast, linkage of a sugar to the C1 position of sulfated galactose should not generally affect binding to Cys-MR. Thus, Cys-MR binds chondroitin sulfates that have a terminal 4-sulfated GalNAc with a β1-4 linkage to iduronic acid or to glucuronic acid and also to sulfated blood group carbohydrates with a terminal 3-sulfated galactose joined with a β1-4 or β1-3 linkage to N-acetylglucosamine 19. As the hydroxyl group attached to C1 is exposed to solvent in the Cys-MR/4-SO4-GalNAc structure and is predicted to be exposed on 3-SO4-GalNAc bound to Cys-MR ( Fig. 3 A), it should be possible to link another sugar ring at this position without creating steric hindrance. Thus, it is predicted that the sugar adjoining the terminal sulfated sugar would not contact Cys-MR unless it contained bulky substitutions, thus rationalizing the observation that the identity of the sugar linked to the terminal sulfated carbohydrate did not generally affect the ability to bind to Cys-MR 19.
Other ligands of Cys-MR include sulfated oligosaccharides of the blood group Lewisx and Lewisa series. Lewisx and Lewisa saccharides that include a sulfate group at the C3 position of the terminal galactose ring (3-SO4-Lewisx and 3-SO4-Lewisa) bind Cys-MR, with 3-SO4-Lewisx binding slightly (approximately three-fold) better than 3-SO4-Lewisa 19. To model the interaction between Cys-MR and sulfated Lewisx, we used a model of 3-SO4-Lewisx derived from the structures of Lewisx alone 46 and sulfated Lewisx bound to a selectin-like mutant of mannose-binding protein 47. The structure of 3-SO4-Lewisa was modeled from the 3-SO4-Lewisx structure as described 48. The 3-SO4-galactose portions of 3-SO4-Lewisx and 3-SO4-Lewisa ( Fig. 3 B) were superimposed with the model of 3-SO4-GalNAc bound to Cys-MR ( Fig. 3 A), allowing rotational freedom about the sulfo–galactose bond. In the case of 3-SO4-Lewisx, a stacking interaction between Trp117, the sulfated galactose ring, and the fucose ring is predicted ( Fig. 3 B). When 3-SO4-Lewisa is positioned similarly in the binding site, the N-acetyl portion of the glucose ring of Lewisa clashes with Cys-MR residue Asn102 ( Fig. 3 B), which may account for the observed preference for 3-SO4-Lewisx over 3-SO4-Lewisa 19.
|
Discussion |
|---|
|
TopAbstractIntroductionMaterials and MethodsResultsDiscussionReferences |
|---|
The two types of carbohydrate-binding regions of MR have different structures and sugar-binding properties. The eight CRDs of MR are predicted to fold into structures resembling C-type lectin domains 4 14, as recently confirmed by the crystal structure of MR CRD4. The CRD fold contains -helical and β-strand secondary structural elements and regions with no ordered secondary structure that include the calcium-binding loops 14 42. Calcium is required for the integrity of the fold and for binding carbohydrates, such that a calcium ion forms direct coordination bonds with mannose, fucose, N-acetylglucosamine, and glucose molecules. Protein–ligand interactions in CRDs include hydrogen bonds between the sugar hydroxyls and protein side chains 14 42. The fold of the NH2-terminal Cys-MR region, the other carbohydrate-binding portion of MR, bears no resemblance to CRDs. Instead, Cys-MR is a member of the β-trefoil family of proteins, which are approximately three-fold symmetric structures composed of 12 β strands 34. Other β-trefoil proteins, such as acidic FGF (aFGF) and the trefoil region of the ricin B chain, bind carbohydrates, but their binding sites and mechanisms of carbohydrate recognition differ from those of Cys-MR ( Fig. 1 A). Thus, the Cys-MR binding site and residues critical for ligand recognition could not have been predicted from structural comparisons, but were revealed in the crystal structure of Cys-MR complexed with 4-SO4-GalNAc. The binding site is a neutral pocket that accommodates the sulfate group, with numerous hydrogen bonds between the sulfate oxygens and protein atoms, primarily main chain NH groups, which serve to disperse the negative charge. Although the galactose ring of the ligand makes specific contacts with Cys-MR, the majority of interactions involve the sulfate group, rationalizing the specificity of Cys-MR for acidic carbohydrate ligands such as sulfated galactose 4.
Identification of the residues in the Cys-MR binding site facilitates comparisons with the sequences of cysteine-rich regions of other proteins in the MR family. The cysteine-rich domains of other members of the MR family, including DEC-205 10 and the phospholipase A2 receptor 7 8 9, contain a deletion in their sequences in the region analogous to that used by Cys-MR for binding 4-SO4-GalNAc ( Fig. 1 C). Thus, these receptors are not predicted to bind sulfated carbohydrates using in a manner analogous to Cys-MR, consistent with the observation that this region of DEC-205 does not bind lutropin 19. Together with the structure of CRD4, the structure of Cys-MR provides a structural framework to evaluate sugar recognition by the two types of carbohydrate-binding portions of the MR family of proteins.
|
Acknowledgments |
|---|
This work was supported by the Howard Hughes Medical Institute (P.J. Bjorkman and M.C. Nussenzweig) and grants from the National Institutes of Health (M.C. Nussenzweig). Y. Liu was a recipient of Ferguson Fund predoctoral Fellowship and Teaching Assistantship (California Institute of Technology).
Submitted: 7 December 1999
Revised: 27 January 2000
Accepted: 4 February 2000
Feinber, H., S. Park-Snyder, A.R. Kolatkar, C.T. Heise, M.E. Taylor, and W.I. Weis, manuscript submitted for publication.
|
References |
|---|
|
TopAbstractIntroductionMaterials and MethodsResultsDiscussionReferences |
|---|
Janeway C.A. Jr.. The immune system evolved to distinguish infectious nonself from non-infectious self, Immunol. Today., 13, 1992, 11–16 .[Medline]
Takahashi K., Donovan M.J., Rogers R.A. & Ezekowitz R.A.B.. Distribution of murine mannose receptor expression from early embryogenesis through to adulthood, Cell Tissue Res., 292, 1998, 311–323 .[Medline]
Linehan S.A., Martínez-Pomares L., Stahl P.D. & Gordon S.. Mannose receptor and its putative ligands in normal murine lymphoid and nonlymphoid organsin situ expression of mannose receptor by selected macrophages, endothelial cell, perivascular microglia, and mesangial cells, but not dendritic cells, J. Exp. Med., 189, 1999, 1961–1972 .
Stahl P.D. & Ezekowitz R.A.B.. The mannose receptor is a pattern recognition receptor involved in host defense, Curr. Opin. Immunol., 10, 1998, 50–55 .[Medline]
Sallusto F., Cella M.I., Danieli C. & Lanzavecchia A.. Dendritic cells use macropinocytosis and the mannose receptor to concentrate macromolecules in the major histocompatibility complex class II compartmentdownregulation by cytokines and bacterial products, J. Exp. Med., 182, 1995, 389–400 .
Prigozy T.I., Sieling P.A., Clemens D., Stewart P.L., Behar S.M., Porcelli S.A., Brenner M.B., Modlin R.L. & Kronenberg M.. The mannose receptor delivers lipoglycan antigens to endosomes for presentation to T cells by CD1b molecules, Immunity., 6, 1997, 187–197 .[Medline]
Ishizaki J., Hanasaki K., Higashino K., Kishimo J., Kikuchi N., Ohara O. & Arita H.. Molecular cloning of pancreatic group I phospholipase A2 receptor, J. Biol. Chem., 269, 1994, 12156–12162.
Lambeau G., Ancian P., Barhanin J. & Lazdunski M.. Cloning and expression of a membrane receptor for secretory phospholipases A2, J. Biol. Chem., 269, 1994, 1575–1578 .
Ancian P., Lambeau G., Madei M.-G. & Lazdunski M.. The human 180 kDa receptor for secretory phospholipases A2, J. Biol. Chem., 270, 1995, 8963–8970 .
Jiang W.P., Swiggard W.J., Heufler C., Peng M., Mirza A., Steinman R.M. & Nussenzweig M.C.. The receptor DEC-205 expressed by dendritic cells and thymic epithelial cells is involved in antigen processing, Nature., 375, 1995, 151–155 .[Medline]
Wu K., Yuan J. & Lasky L.A.. Characterization of a novel member of the macrophage mannose receptor type C lectin family, J. Biol. Chem., 271, 1996, 21323–21330 .
Taylor M.E., Bezouska K. & Drickamer K.. Contribution to ligand binding by multiple carbohydrate recognition domains in the macrophage mannose receptor, J. Biol. Chem., 267, 1992, 1719–1726 .
Taylor M.E. & Drickamer K.. Structural requirements for high affinity binding of complex ligands by the mannose receptor, J. Biol. Chem., 268, 1993, 399–404 .
Weis W.I., Taylor M.E. & Drickamer K.. The C-type lectin superfamily in the immune system, Immunol. Rev., 163, 1998, 19–34 .[Medline]
Mullin N.P., Hitchen P.G. & Taylor M.E.. Mechanism of Ca2+ and monosaccharide binding to a C-type carbohydrate-recognition domain of the macrophage mannose receptor, J. Biol. Chem., 272, 1997, 5668–5681 .
Fiete D. & Baenziger J.U.. Isolation of the SO4-4-GalNAcβ1, 4GlcNAcβ1, 2Man-specific receptor from rat liver, J. Biol. Chem., 272, 1997, 14629–14637 .
Fiete D., Beranek M.C. & Baenziger J.U.. The macrophage endothelial cell mannose receptor cDNA encodes a protein that binds oligosaccharides terminating with SO4-4-GalNAcβ1,4GlcNAcβ or Man at independent sites, Proc. Natl. Acad. Sci. USA., 94, 1997, 11256–11261 .
Fiete D.J., Beranek M.C. & Baenziger J.U.. A cysteine-rich domain of the "mannose" receptor mediates GalNAc-4-SO4 binding, Proc. Natl. Acad. Sci. USA., 95, 1998, 2089–2093 .
Leteux C., Chai W., Loveless R.W., Yuen C.-T., Uhlin-Hansen L., Combarnous Y., Jankovic M., Maric S.C., Misulovin Z., Nussenzweig M.C. & Feizi T.. The cysteine-rich domain of the macrophage mannose receptor is a multispecific lectin which recognizes chondroitin sulfates A and B and sulfated oligosaccharides of blood-group Lewisa and Lewisx types in addition to the sulfated N-glycans of luteinizing hormone, J. Exp. Med., 191, 2000, 1117–1126 .
Baenziger J.U., Kumar S., Brodbeck R.M., Smith P.L. & Beranek M.C.. Circulatory half-life but not interaction with the lutropin chorionic-gonadotropin receptor is modulated by sulfation of bovine lutropin oligosaccharides, Proc. Natl. Acad. Sci. USA., 89, 1992, 334–338 .
Smith P.L., Bousfield G.R., Kuman S., Fiete D. & Baenziger J.U.. Equine lutropin and chorionic gonadotropin bear oligosaccharides terminating with SO4-4-GalNAc and Sia--2,3Gal, respectively, J. Biol. Chem., 268, 1993, 795–802 .
Martínez-Pomares L., Kosco-Vilbois M., Darley E., Tree P., Herren S., Bonnefoy J.-Y. & Gordon S.. Fc chimeric protein containing the cysteine-rich domain of the murine mannose receptor binds to macrophages from splenic marginal zone and lymph node subcapsular sinus and to germinal centers, J. Exp. Med., 184, 1996, 1927–1937 .
Taylor M.E., Conary J.T., Lennartz M.R., Stahl P.D. & Drickmer K.. Primary structure of the mannose receptor contains multiple motifs resembling carbohydrate-recognition domains, J. Biol. Chem., 265, 1990, 12156–12162 .
Otwinowski Z. & Minor W.. Processing of X-ray diffraction data collected in oscillation mode, Methods Enzymol., 276, 1997, 307–326.
De La Fortelle E. & Bricogne G.. Maximum-likelihood heavy-atom parameter refinement for multiple isomorphous replacement and multiwavelength anomalous diffraction methods, Methods Enzymol., 276, 1997, 472–494.
Abrahams J.P. & Leslie A.G.W.. Methods used in the structure determination of bovine mitochondrial F1 ATPase, Acta Crystallogr. D., 52, 1996, 30–42 .[Medline]
Kleywegt G.J. & Jones T.A.. Template convolution to enhance or detect structural features in macromolecular electron density maps, Acta Crystallogr. D., 53, 1997, 179–185 .[Medline]
Jones T.A. & Kjeldgaard M.. Electron density map interpretation, Methods Enzymol., 277, 1997, 173–208.[Medline]
Brünger A.T., Adams P.D., Clore G.M., Gros P., Grosse-Kunstleve R.W., Jiang J.-S., Kuszewski J., Nilges M., Pannu N.S. & Read R.J.. Crystallography and NMR systema new software system for macromolecular structure determination, Acta Crystallogr. D., 54, 1998, 905–921 .[Medline]
Brünger A.T.. Free R valuea novel statistical quantity for assessing the accuracy of crystal structures, Nature., 355, 1992, 472–475.[Medline]
Kraulis P.J.. MOLSCRIPTa program to produce both detailed and schematic plots of protein structures, J. Appl. Crystallogr., 24, 1991, 946–950.
Merritt E.A. & Murphy M.E.P.. Raster3D version 2.0, a program for photorealistic molecular graphics, Acta Crystallogr. D., 50, 1994, 869–873 .[Medline]
Nicholls A., Bharadwaj R. & Honig B.. GRASPgraphical representation and analysis of surface properties, Biophys. J., 64, 1993, A166 .
Murzin A.G., Lesk A.M. & Chothia C.. Beta-trefoil fold patterns of structure and sequence in the Kunitz inhibitors, interleukins-1β and 1 and fibroblast growth factors, J. Mol. Biol., 223, 1992, 531–543 .[Medline]
McLachlan A.D.. Three-fold structural pattern in the soybean trypsin inhibitory (Kunitz), J. Mol. Biol., 133, 1979, 557–563 .[Medline]
Rutenber E. & Robertus J.D.. Structure of ricin B-chain at 2.5 Å resolution, Proteins., 10, 1991, 260–269 .[Medline]
Zhu X., Komiya H., Chirino A., Faham S., Fox G.M., Arakawa T., Hsu B.T. & Rees D.C.. Three-dimensional structures of acidic and basic fibroblast growth factors, Science., 251, 1991, 90–93 .
Finzel B.C., Clancy L.L., Holland D.R., Muchmore S.W., Watenpaugh K.D. & Einspahr H.M.. Crystal structure of recombinant human interleukin-1β at 2.0 Å resolution, J. Mol. Biol., 209, 1989, 779–791 .[Medline]
Graves B.J., Hatada M.H., Hendrickson W.A., Miller J.K., Madison V.S. & Satow Y.. Structure of interleukin-1 at 2.7 Å resolution, Biochemistry., 29, 1990, 2679–2684 .[Medline]
Habazetti J., Gondol D., Wiltscheck R., Otlewski J., Schleicher M. & Holak T.A.. Structure of hisactophilin is similar to interleukin-1β and fibroblast growth factor, Nature., 359, 1992, 855–858 .[Medline]
Harris N., Peters L.L., Eicher E.M., Rits M., Raspberry D., Eichbaum Q.G., Super M. & Ezekowitz R.A.. The exon-intron structure and chromosomal localization of the mouse macrophage mannose receptor gene Mrc1identification of a ricin-like domain at the N-terminus of the receptor, Biochem. Biophys. Res. Commun., 198, 1994, 682–692 .[Medline]
Weis W.I. & Drickamer K.. Structural basis of lectin-carbohydrate recognition, Annu. Rev. Biochem., 65, 1996, 441–473 .[Medline]
Pflugrath J.W. & Quiocho F.A.. The 2 Å resolution structure of the sulfate-binding protein involved in active transport in Salmonella typhimurium, J. Mol. Biol., 200, 1988, 163–180 .[Medline]
Quiocho F.A., Sack J.S. & Vyas N.K.. Stabilization of charges on isolated ionic groups sequestered in proteins by polarized peptide units, Nature., 329, 1987, 561–564 .[Medline]
DiGabriele A.D., Lax I., Chen D.I., Svahn C.M., Jaye M., Schlessinger J. & Hendrickson W.A.. Structure of a heparin-linked biologically active dimer of fibroblast growth factor, Nature., 393, 1998, 812–817 .[Medline]
Perez S., Mouhous-Riou N., Nifant'ev N.E., Tsvetkov Y.E., Bachet B. & Imberty A.. Crystal and molecular structure of a histo-blood group antigen involved in cell adhesionthe Lewisx trisaccharide, Glycobiology., 6, 1996, 537–542 .
Ng K.K.-S. & Weis W.I.. Structure of a selectin-like mutant of mannose binding protein complexed with sialylated and sulfated Lewisx oligosaccharides, Biochemistry., 36, 1997, 979–988 .[Medline]
Kogelberg H., Frenkiel T.A., Homans S.W., Lubineau A. & Feizi T.. Conformational studies on the selectin and natural killer cell receptor ligands sulfo- and sialyl-lacto-N-fucopentaoses (SuLNFPII and SLNFPII) using NMR spectroscopy and molecular dynamics simulations. Comparisons with the nonacidic parent molecule LNFPII, Biochemistry., 35, 1996, 1954–1964 .[Medline]
Simpson D.Z., Hitchen P.G., Elmhirst E.L. & Taylor M.E.. Multiple interactions between pituitary hormones and the mannose receptor, Biochem. J., 343, 1999, 403–411 .[Medline]
Martínez-Pomares L., Mahoney J.A., Káposzta R., Linehan S.A., Stahl P.D. & Gordon S.. A functional soluble form of the murine mannose receptor is produced by macrophages in vitro and is present in mouse serum, J. Biol. Chem., 273, 1998, 23376–23380 .
Hodel A., Kim S.-H. & Brünger A.T.. Model bias in macromolecular crystal structures, Acta Crystallogr. A., 48, 1992, 851–858.
Kolatkar A.R. & Weis W.I.. Structural basis of galactose recognition by C-type animal lectins, J. Biol. Chem., 271, 1996, 6679–6685.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Facebook 
Reddit 
Technorati 
Twitter What's this?
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| TABLE OF CONTENTS |
|
