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Original Article |
ebutcher{at}stanford.edu
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Key Words: receptors, lymphocyte homing • receptors, chemokine • cell adhesion • endothelium, vascular • lymphoid tissue
Recent evidence has highlighted the potential role in lymphocyte migration and homing of a relatively new class of soluble leukocyte chemoattractants called chemokines, which signal through PTX-sensitive G
Our results demonstrate that SLC is displayed by a subset of HEVs that support T cell arrest, and that absence of SLC presentation by HEVs or functional inhibition of the SLC/MIP-3β receptor CCR7 abrogates the firm arrest of T but not B cells in PP-HEVs. Together, these data suggest a critical role for CCR7 in T cell–HEV recognition, and reveal a fundamental difference in the signaling requirements for T and B cell recruitment to PP-HEVs. Furthermore, our studies demonstrate an unexpected segregation of HEV sites of T and B cell accumulation within PPs, correlating with differential chemokine display in well-defined microenvironmental domains. These results suggest a novel mechanism for the microenvironmental targeting of lymphocyte subsets, at the level of HEV recognition and recruitment from the blood.
mAbs.
Lymphocyte Isolation and Treatment.
Animals.
PP Preparation and Intravital Microscopy.
Analysis of In Situ Lymphocyte Behavior in PP-HEVs.
The total number of cells entering HEVs in the blood per minute was defined as the total cell flux. Cells were considered noninteracting when they moved at the velocity of RBCs, appearing as streaks on videotape images. These were easily distinguished from rolling cells, whose velocity is typically 10-fold lower: these rolling cells appear as circles on each video frame, and were characterized by velocities in our experiments <300 µm/s (generally 20–150 µm/s, similar to previous reports [2, 3, 43]). Cells were considered interacting whether they rolled, arrested, or both. The rolling fraction is the percentage of cells that interact with a given venule in the total number of cells that enter that venule during the same period. Velocities of >15 consecutive rolling lymphocytes were calculated as described 1. Sticking/arrest or firm adherence was defined as interacting cells that become immobile for >3 s 1. Total flux and rolling fraction data were collected during a period 4–8 min after injection, and represent at least 10 consecutive cells in at least 10 HEVs per preparation. Accumulated cells (i.e., cells that did not move during a 30-s observation) in the same HEV were enumerated at the 8-min time point. The values of total flux, rolling fraction, or accumulated cell numbers for the experimental population were normalized to those of the coinjected internal standard cells assessed within the HEV and time period, thus controlling for natural variability in hemodynamics and vascular characteristics. The resulting ratio provides an index of the behavior of the chemokine-treated test cells (e.g., the total flux of test cells divided by the flux of internal standard cells), and this index is then expressed as a percentage of that of the index of control (mock-treated) experimental cell flux/internal standard cell flux determined in parallel littermate animals. The control experiments using the mock-treated cells were performed immediately before and after those using chemokine treatments. The use of internal standard populations, and the analysis of data and results of in vivo homing data in this manner have been discussed in more detail elsewhere 12.
Statistical Methods.
Desensitization with CCR7 ligand SLC, but not with the CXCR4 ligand SDF-1
Introduction
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Abstract
Introduction
Materials and Methods
Results
Discussion
References
Recirculating naive lymphocytes are recruited from the blood stream into the secondary lymphoid tissues of Peyer's patches (PPs) and LNs via selective interactions with high endothelial venules (HEVs). Lymphocyte recognition of HEVs involves a multistep adhesion cascade in which primary adhesive interactions (rolling) are followed by firm arrest (sticking) in response to pertussis toxin (PTX)-sensitive rapid signaling events ( 1 2 3; for a review, see reference 4). Although the functional contribution of endothelial and lymphocytic adhesion molecules has been characterized 1 2 3 4 5, the identity and physiological roles of the signaling receptor(s) and their ligand(s) involved in lymphocyte–HEV recognition have not been defined. 
i-linked serpentine receptors 6 7. PPs and peripheral LNs (PLNs) express message RNA for at least three chemokines, including secondary lymphoid tissue chemokine (SLC) and macrophage inflammatory protein (MIP)-3β, which act through a common CC chemokine receptor, CCR7; and stromal cell–derived factor (SDF)-1, which signals through CXC chemokine receptor (CXCR)4; each of which attracts the majority of lymphocytes 8 9 10 11, including the naive cells that characteristically home to secondary lymphoid organs 12. We and others have hypothesized that CCR7 on lymphocytes may be critical for lymphocyte–HEV interactions because CCR7 ligands are most abundant in secondary lymphoid tissues and, in particular, because SLC message is expressed by endothelial cells of HEVs 10. Furthermore, recent in vitro studies have demonstrated rapid adhesion and transendothelial chemotaxis of B and T cells in response to CCR7 ligands in assays that model in vivo shear and adhesive environments of secondary lymphoid tissues 8 10 14 15. To explore the involvement of CCR7 and its ligands in physiological lymphocyte interactions with PP-HEVs, we selectively attenuated CCR7 function in lymphocytes by ligand desensitization, observed their interactions in situ using intravital microscopy, and compared their behavior with that of normal lymphocytes interacting with SLC-deficient HEVs in DDD/1 mice. In addition, we assessed the in situ display of CCR7 ligand SLC by PP-HEVs using confocal microscopy, compared SLC localization with sites of lymphocyte accumulation, and investigated the differential accumulation of T and B cells in PP-HEVs.
Materials and Methods
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Reagents.
CellTrackerTM Green, 5-chloromethylfluorescein diacetate (CMFDA; Molecular Probes, Inc.) was prepared as a 0.5 mg/ml stock solution in DMSO, stored at –30°C, and used within 1 wk. Tetramethylrhodamine-5-(and-6)-isothiocyanate (5[6]-TRITC; Molecular Probes, Inc.) was prepared as a 2 mM solution in DMSO, diluted to a stock 1:10 with saline, sterile filtered, and stored in single aliquots at –30°C. AlexaTM 488 and AlexaTM 546 protein labeling kits were used as directed by the manufacturer (Molecular Probes, Inc.). Chemokines mouse SLC (Exodus-2/thymus-derived chemotactic agent 4 [TCA-4]/6Ckine; a gift of DNAX Research Institute), human MIP-3β (ELC), and human SDF-1 (PeproTech) were prepared as 0.5 mg/ml stock solutions in saline and used immediately, or stored as aliquots at –80°C. Labeling medium (LM) was 50% RPMI 1640 and 50% Ca/Mg-free HBSS (BioWhittaker), supplemented with 1.5% bovine calf serum and 10 mM Hepes (cRPMI) (Sigma Chemical Co.) and was made fresh daily.
mAbs Mel-14 (rat IgG2a, anti–mouse L-selectin 16), MECA 79 (rat IgM, anti–peripheral node addressin [PNAd]), MECA 367 and MECA 89 (rat IgM, anti–mucosal addressin cell adhesion molecule [MAdCAM]-1 17), 9B5 (rat IgM, anti–human CD44), and 4B1E7C10 (hamster IgG, anti–mouse SLC [18]) were generated in our laboratories. Hybridoma cells for these mAbs as well as mAbs UC8-1B9 (hamster IgG, anti-dinitrophenol [DNP]; American Type Culture Collection), Tib 213 (rat IgG2b, anti–mouse CD11a; American Type Culture Collection 19), and PS/2 (rat IgG2b, anti–mouse CD49d; American Type Culture Collection) were purified from culture supernatant. mAbs were stored at –80°C in endotoxin-free saline at 1 mg/ml. For in situ staining studies, 12–75 µg of antibody was injected via left carotid arterial cannulation. FITC-, AlexaTM 488–, and AlexaTM 546–conjugated mAbs to DNP, human CD44, mouse L-selectin, mouse CD11a, mouse CD49d, mouse SLC, and mouse MAdCAM-1 were made in our laboratories. FITC-conjugated mAbs to murine CD3 and B220 were from PharMingen.
LN cells (LNCs) were freshly isolated from PLNs (axillary, brachial, cervical, and subiliac) and mesenteric LNs (MLNs). Splenocytes were freshly isolated from disaggregated spleens, and cleared of red blood cells using a Whole Blood Erythrocyte Lysing kit (R&D Systems). Cells were incubated for 1 h at 37°C in cRPMI, then washed and resuspended to 1.5 x 107 cells/ml in warm LM. Representative samples were stained with FITC-conjugated mAbs to T and B cell markers (CD3 and B220, respectively) and determined by flow cytometry to be 
95% lymphocytes. For isolation of T and B cell populations, negative selection columns were used as directed by the manufacturer (R&D Systems, and Cedarlane). Purity as determined by CD3 or B220 staining was on average >96% for T cells, and >90% for B cells. For fluorescent labeling, 2 ml LNCs was incubated with 4 µM CMFDA or 0.8 µM TRITC in LM for 10 min at 37°C. Lymphocytes were centrifuged through 2 ml of warm bovine calf serum, and supernatant was discarded. The pellet was resuspended to 1.5 x 107 cells/ml in warm LM. Aliquots of LNCs were treated with chemokine or saline for 45 min, washed again through serum, resuspended at 1 x 108 cells/ml in warm saline, and used immediately.
We used adult BALB/c and DDD/1 23 mice (bred in the animal facility of Palo Alto Veterans Medical Center) of both sexes (10–14 wk, 18–25 g body wt) as recipients for in vivo experiments, and young BALB/c mice (6–10 wk, 12–20 g body wt) as lymphocyte donors. All experimental procedures comply with National Institutes of Health guidelines for the care and use of laboratory animals, and were approved by the Standing Committees on Animals of Stanford Medical School and The Palo Alto Veterans Medical Center. Animals were under complete surgical anesthesia throughout all experimental procedures.
Mice were anesthetized by intraperitoneal injection (10 ml/kg) of physiologic saline containing xylazine (1 mg/ml) and ketamine (5 mg/ml). A loop of small intestine displaying at least one PP was prepared for intravital microscopy as described previously 1 2 with modifications. In brief, a polyethylene catheter was inserted in the left carotid artery and was used for injection of cell suspensions and mAbs. A midline incision was made to expose the peritoneal cavity, and a jejunal or duodenal loop was exposed and arranged within a rubber O-ring upon a small raised platform attached to a stage, and kept moist with warm saline. After the PP to be examined was positioned, a glass coverslip was applied to enclose the preparation for microscopic viewing. The stage was then placed on a Nikon intravital microscope equipped with a silicon-intensified target camera (Cohu, Inc.). The PP was visualized by epifluorescence from a mercury lamp source. Video images were recorded using a time-base generator (For-A) and a VHS VCR (JVC). Venular segments to be video-recorded were located by the characteristic autofluorescence of stromal elements in PP primary follicles, and identified by characteristic physical structure.
Boluses (100–200 µl) of fluorescently labeled LNCs or splenocytes (108 cells/ml) were retrogradely injected via the carotid artery catheter over 30 s. Lymphocyte behavior in PLNs was recorded through 16x Neofluor (Nikon) or 20x water immersion objective (Achroplan; Carl Zeiss, Inc.). Cell behavior in individual vessel segments was determined by playback of videotapes. For each experiment, cells of one color were selected as the experimental population, and these cells were resuspended after fluorescent labeling to 1.5 x 107/ml, aliquoted into two tubes, and incubated with chemokine (desensitized test cells) or control medium (mock-treated control cells) for 45 min at 37°C, then washed by centrifugation through a serum cushion. Cells of the other color, used as an internal standard population, were similarly washed after labeling, then mixed at 1:1 ratio with the experimental test or control cells at a final concentration of 108 cells/ml in warm saline. (Aliquots of injected mixtures were saved for FACS® analyses to determine the precise ratio of experimental to standard cells injected.) Cells were then immediately injected via carotid artery cannula into mice prepared for intravital microscopy. Areas within the selected PPs were surveyed in the initial 2 min for HEVs supporting lymphocyte interactions, and then observed for an additional 10 min. Microscope filter sets were alternated every 30 s to allow observations of differently labeled (experimental versus internal standard) cells, thus allowing comparison of behavior of identically labeled test (chemokine-treated) cells versus mock-treated (control) cells, in littermate animals, with the behavior of the same internal standard cell population.
Data in graphs are shown as means ± SD, unless otherwise indicated. For statistical comparison of two samples, a two-tailed Student's t test was used when applicable. Differences were considered statistically significant when P < 0.05.
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Chemokine-desensitized Lymphocyte Interactions in PP-HEVs.
Lymphocytes exposed to high concentrations of a chemokine become refractory to subsequent stimulation with the same chemokine, or other agonists that signal through the same receptor(s). This selective unresponsiveness is thought to be mediated by the well-described phenomenon of ligand-induced homologous receptor desensitization 20 21. We have previously shown that SLC and MIP-3β can desensitize mouse LNCs, as well as human PBLs, and that these chemokines signal via the same chemokine receptor, CCR7 22. Here we asked whether desensitization of lymphocytes with SLC, which is expressed by HEVs and can trigger rapid integrin-mediated adhesion in vitro, could inhibit in situ signals that trigger integrin-mediated firm adhesion of rolling lymphocytes. We observed the behavior of chemokine-treated or control lymphocytes within PP-HEVs, while recording video images for later analysis. Unfractionated, monocyte-depleted LNCs derived from PLNs and MLNs were fluorescently labeled, then pretreated with chemokine or control media before use. Cells were washed through a warm serum gradient and immediately injected into a mouse prepared for intravital microscopy. , substantially inhibits accumulation of intravenously injected LNCs in PP-HEVs in vivo ( Fig. 1). Total flux and rolling fraction of SLC-treated lymphocytes through HEV were, if anything, slightly increased over control. Rolling velocity ( Fig. 1 legend) and surface levels of adhesion receptors L-selectin, LFA-1, and 4β7 on desensitized lymphocytes (data not shown) remained unchanged from mock-treated control. Chemokine desensitization dramatically reduced the conversion of rolling to sticking behavior and firm arrest, an effect that is similar to PTX treatment and consistent with inhibition of the specific Gi protein–linked signaling event required for integrin-mediated activated rapid adhesion.
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i heterotrimeric G protein–mediated signaling event (data not shown). Together, these results suggest that B cell firm arrest in HEVs is triggered by a chemokine (or other chemoattractant) receptor independent of or in addition to CCR7.
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, and their receptors.
Microenvironmental Control of Lymphocyte Recruitment.
We wished to address further the role of SLC, speculating that for SLC to trigger adhesion of blood-borne lymphocytes, it must be presented on the lumen of HEVs. We intravenously injected fluorescently labeled anti–mouse SLC mAbs, anti–MAdCAM-1 mAbs, or control mAbs into normal BALB/c mice for intravital staining of vessel endothelium ( Fig. 5). Anti-SLC reacted with most MAdCAM-1+ HEVs in PPs, but reactivity was variable in different regions. The most intensely staining segments were predominately located within interfollicular areas, whereas MAdCAM-1+ HEVs lacking detectable SLC were most common in or adjacent to follicles. Presentation of SLC by segments of venular trees draining individual follicles was also variable, particularly in older mice. Anti-SLC reactivity appeared stronger in PPs of young mice, and rarely, a small section of a precapillary arteriole would display some anti-SLC reactivity (our unpublished observations). Despite high levels of injected labeled mAb, we were unable to detect any SLC on PP-HEVs of DDD/1 mice ( Fig. 5).
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i-mediated signals trigger integrin-dependent sticking of rolling lymphocytes, and likely participate in their diapedesis and localization within tissue microenvironments. Recently, chemokines have been highlighted as prime candidates that provide PTX-sensitive signals mediating lymphocyte–endothelial recognition and directed migration. Here, we have investigated the role of known rapid adhesion-triggering chemokines in the recognition of PP-HEVs by circulating B and T lymphocytes. Our data suggest that naive T cells rely upon recognition of luminally presented SLC and use signals mediated by CCR7 to arrest firmly in PP-HEVs. In contrast, B cells, though capable of responding to SLC, use distinct (or additional) mechanisms to arrest in PP-HEVs. Furthermore, we present evidence that differential display of SLC and B cell adhesion-triggering factors by interfollicular versus follicle-associated high endothelium allows microenvironmental control of T versus B cell recruitment from the blood. With the results presented here, the evidence for CCR7 and SLC involvement in T cell recognition of HEVs seems compelling. Previous studies have shown that HEVs express message for SLC 10, that SLC can trigger rapid integrin-dependent arrest of naive B and T lymphocytes under physiologic shear in vitro 8 22, and that a selective defect in T cell homing to LNs in DDD/1 mice is associated with deficient SLC and MIP-3β expression 24. Here we show that HEVs in fact display SLC protein, that desensitization of CCR7-mediated signaling with SLC (or MIP-3β) inhibits T cell arrest, and that T cell accumulation correlates with sites of SLC expression. Moreover, we show that the T cell homing deficiency in DDD/1 mice involves a lack of T cell adhesion-triggering responses on HEVs, a phenotype indistinguishable from that of SLC- or MIP-3β–desensitized T cells in wild-type hosts. We conclude that SLC and its receptor CCR7 can mediate T cell recognition of HEVs in lymphoid organs.
These studies provide the first direct evidence of chemokine involvement in adhesion triggering during physiologic lymphocyte–endothelial interactions, thus confirming the hypothesized role of chemokines in lymphocyte–endothelial cell recognition 25. Chemokine-triggered adhesion may be part of a general paradigm in which functionally distinct subsets of circulating lymphocytes use specialized chemokine receptors, in place of or in addition to CCR7, for homing from the blood, especially in settings of inflammation. A significant subset of circulating human memory T cells expresses CCR7 ( 26; and Campbell, J.J., and L. Wu, unpublished observation), both human and mouse memory T cells respond to SLC and MIP-3β 11 13 18 22 27 28, and recent evidence indicates increased expression of CCR7 and concomitant greater response to SLC after activation 13 14. Thus, memory cells may also use CCR7, like naive T cells, during recirculation through organized lymphoid tissues. Display of other chemokine receptors, such as CXCR3, G protein–coupled receptor GPR-9-6 (CCR9), CCR4, CCR5, and CCR6, also subdivides circulating memory cells, and may help confer unique vascular recognition and tissue- or inflammation-selective homing properties. For example, recent studies suggest a physiological role for CCR4 on systemic memory T cells, and its ligand thymus and activation-regulated chemokine (TARC) displayed by inflamed venules, in the adhesion-triggering step of memory lymphocyte homing to cutaneous sites of inflammation 29, and potentially for CCR9 and its ligand thymus-expressed chemokine (TECK) in intestinal lymphocyte homing 30.
Although our studies implicate SLC receptor(s) in T cell–HEV interactions, and demonstrate SLC presentation by HEV segments mediating T cell accumulation, they do not exclude involvement of MIP-3β (or other unidentified CCR7 ligands) in T cell arrest. Like SLC, MIP-3β has potent chemotactic activity for naive T and B cells, and for subsets of memory CD4 and CD8 cells, but not for granulocytes and monocytes 11 22 27 28. Importantly, MIP-3β acts through the same receptor as SLC, CCR7, can cross-desensitize CCR7 to further signals from either MIP-3β or SLC 22 31, and, as we have shown here, desensitization with MIP-3β selectively prevents accumulation of T but not B cells in PP-HEVs. MIP-3β is expressed in lymphoid tissues, notably in parafollicular and inner cortical regions of LNs, and by LN stromal elements including dendritic cells derived from T cell zones 11 32. Although in situ hybridization studies suggest that MIP-3β is not produced by endothelium of HEVs, it is possible that luminal presentation may occur by transcytosis from surrounding tissue. Indeed, findings in other systems demonstrate that chemokines can gain access to the venular lumen even when expressed or introduced in a perivascular location 33. HEV presentation of MIP-3β by this mechanism may account for our inability to block T cell arrest in PP-HEVs with bolus injections of anti-SLC mAbs: intravenous injection of up to 1 mg per mouse yielded only variable and incomplete inhibition of T cell accumulation, never exceeding 50% of control (our unpublished results). However, this may also reflect incomplete blockade of SLC in vivo, as anti-SLC mAbs accumulate only slowly on HEVs (over minutes) compared with anti–MAdCAM-1 mAbs or other anti-endothelial cell antibodies we have used for in situ endothelial visualization. Resolution of the role of MIP-3β or other CCR7 ligands in addition to SLC will require generation of additional, high-affinity anti-SLC and MIP-3β mAbs, and/or evaluation of single and dual gene-targeted mice in future studies.
L-selectin, which is rapidly shed upon cross-linking, has also been shown to transmit signals that can affect integrin affinity 34. Naive B and T lymphocytes interacting with PP-HEVs rely on L-selectin for adequate attachment, and to some degree, for rolling 2 35. Although chemokine desensitization of T lymphocytes with SLC did not affect attachment and rolling, sticking was abrogated, suggesting that L-selectin signals, if operating in this context, are insufficient to mediate arrest. While we cannot rule out a contribution by L-selectin signaling, particularly for B cells, it is unlikely to be sufficient for physiological adhesion-triggering, given that lymphocyte arrest is PTX sensitive, whereas L-selectin signaling is not 36.
Although we have not here evaluated lymphocyte interactions in situ with LN HEVs, in the course of our studies we noted that some PLN HEVs display SLC, and that desensitization with SLC or MIP-3β prevents accumulation of injected T lymphocytes in LN as well as PP-HEVs. In conjunction with the known deficiency in T cell homing to LNs in DDD/1 mice, these observations suggest that CCR7 and its ligands play comparable roles in T cell adhesion triggering in LNs as in PPs. This postulate has been confirmed in parallel studies from Stein and coworkers 36a.
One of the most interesting discoveries here is the differential vascular interactions of B versus T cells, and their control at the level of adhesion triggering. Accumulation of T and B cell subsets is markedly segregated in PP-HEVs, with T cells collecting preferentially in interfollicular HEV segments, associated with high levels of local SLC expression. Conversely, although B cells can also arrest in SLChi HEVs, they tend to accumulate within intrafollicular HEV segments, or segments immediately adjacent to follicles—segments which are often SLClow/–. This microenvironmental control of T versus B cell interactions with HEVs was surprising in light of earlier models, which assumed that naive B and T cells were recruited equivalently through HEVs, with segregation to their appropriate compartments occurring entirely after entry into the lymphoid tissue parenchyma 37 38. Indeed, earlier studies of B and T cell interactions with HEVs, using either ex vivo Stamper-Woodruff frozen section assays or short-term homing of adoptively transferred lymphocyte subsets, failed to identify differential association of T versus B cells with HEV segments ( 39 40 41 42; and Butcher, E.C. and I.L. Weissman, unpublished results). This likely reflects the techniques employed, which did not allow visualization of homing interactions, or, in the case of frozen section studies, may not have adequately modeled in vivo physiology. However, these prior studies also focused on HEV interactions in PLNs of young mice, in which follicular domains are characteristically less prominent and less immunologically active than in the PPs studied here. To the extent that the HEV segments recruiting B but not T cells are induced or regulated by the follicular microenvironment, as we propose (see below), it seems likely that such segments would be relatively infrequent and capable of less selective B (versus T) cell recruitment in LNs. It is interesting to note that we have also occasionally observed focal segregation of B and T cell accumulation in MLNs and sheep RBC-immunized PLNs, with B greater than T cell recruitment by subcapsular (follicle-associated) HEVs (data not shown). (In these organs, such segregation is less prominent than in PPs, suggesting that follicular localization might be directed principally by chemotaxis of B cells after extravasation.) Furthermore, consistent with our findings, another study of PPs noted that B cell arrest occurs in a wider range of vessels (by diameter) than T cells, including smaller venules observed inside follicles 43.
In this context, it is noteworthy that early studies of T versus B cell interaction did reveal a relative preference of B versus T cells for PP (versus PLN) HEVs, both in ex vivo frozen section assays and in short-term in vivo homing studies 40 41. This behavior may be explained in part by the fact that naive B cells display slightly higher levels of the intestinal PP homing receptor 4β7, and slightly lower levels of PLN homing receptor L-selectin, than naive T cells 44 45. However, it is reasonable to propose that a higher level of B cell adhesion-triggering factors in PP-HEVs (relative to PLN HEVs) could also contribute to their selective localization, as well as to the greater frequency of homed B than T cells in PPs.
The physical structure of the vasculature in PPs may also promote the follicular localization of B cells, as PP-HEVs first arise from capillary arcades predominantly in or near B cell follicles, and then drain out through parafollicular T cell zones 46. Therefore, the majority of PP-HEVs that cells first encounter lie within the proximal influence of B cell microenvironments, whereas T cell zones effect control over "downstream" HEV segments. Our results show that B cells accumulate efficiently in the upstream, high order, follicle-associated segments in response to an as yet unidentified adhesion-triggering factor(s). In contrast, although T cells efficiently tether and roll in these high order (predominantly) SLClow segments in and/or near follicles, they arrest only infrequently. Instead, T cells continue rolling downstream to arrest eventually in lower order SLChi HEVs located within the interfollicular zone. B cells that escape early recruitment to the follicular segments can also arrest downstream on SLChi HEVs; whether SLC itself contributes to B cell arrest on these lower order vessels remains to be determined. Thus, the selective homing of T and B cells is determined by their signaling receptors, and not only by disparate levels of counterreceptors for PP-HEV adhesion molecules. Taken together, these studies demonstrate a novel mechanism for the selective recruitment of lymphocyte subsets to specialized lymphoid tissue domains—the differential presentation of factors that trigger firm adhesion.
The segmental control of chemokine expression, as we have demonstrated, is most likely in response to and within the context of microenvironmental cues. PPs and LNs can be extensively remodeled during instances of immune or inflammatory stimulation, and these secondary lymphoid tissues (or their specialized domains) must be capable of rapid and robust architectural refinement, particularly through the specific recruitment of specialized subsets of lymphocytes 47. It will be important to evaluate the extent to which developing lymphoid compartments, or those undergoing acute inflammatory reactions, display altered patterns of chemokine expression on HEVs and within cortical and follicular compartments. Indeed, recent studies of inflamed LNs, PPs, and spleen have demonstrated significant changes in chemokines and adhesion molecules in response to inflammatory challenge, though the relationship to recruitment of lymphocyte subsets has not been examined ( 48 49 50 51 52 53 54 55; and McEvoy, L.M., unpublished observations). Taken together with our findings, such observations suggest that lymphocyte subset recruitment by LN and PP-HEVs may be tightly controlled through elements of the multistep adhesion cascade, and can be dynamically regulated by altered chemokine display.
In addition to its role in T cell recognition of HEVs, as evidenced here, SLC has been hypothesized to direct migration of lymphocytes and also dendritic cells to and within lymphoid and other tissues 10 13 56 57 58. For example, in situ hybridization and immunohistological studies have revealed high levels of SLC message in the endothelium of lymphatic openings and luminal presentation on lymphatic vessels within extralymphoid tissues, suggesting a potential role of SLC and CCR7 in regulating cellular entry into afferent lymph 10 59. Consistent with this, recent studies have shown that activated dendritic cells upregulate CCR7, and have provided evidence that SLC is required for efficient dendritic cell recruitment to draining LNs during inflammation 24 59 60. It is attractive to postulate that CCR7 and SLC may play a similar role for memory or activated lymphocytes in extravascular recruitment to draining LNs via lymphatics. Furthermore, SLC (as well as MIP-3β) is expressed by stromal cells associated with T cell zones in spleen, thymus, and other lymphoid structures 11 13 18 24 61 62. Expression of CCR7 ligands at such sites may serve to recruit or position relevant cells to the appropriate T cell–rich zones, and/or to ensure a close relationship between antigen-presenting stromal cells and naive and antigen-reactive T cells. However, if T cells recruited through SLChi HEVs are transiently desensitized to SLC, as is possible, regulation of diapedesis and homing into T cell zones would likely require additional chemoattractant mechanisms. If this model is correct, one could imagine that the widespread and high level of expression of CCR7 ligands within T cell zones might serve more to prevent inappropriate "wandering" or retrograde migration of T cells back to HEVs, by establishing a uniform or desensitizing field of CCR7 agonists. Thus, a clear picture of the role of SLC or additional chemotactic factors in extravasation and directed migration of lymphocytes awaits further study.
In conclusion, we have provided direct evidence for chemokine regulation of lymphocyte–endothelial recognition in vivo. Our results indicate that T cell arrest on HEVs can be mediated by SLC and requires signaling through the SLC receptor, CCR7, and demonstrate that B and T cells use distinct adhesion-triggering factors for HEV recognition. Furthermore, we have shown that adhesion-triggering factors for B and T cells are differentially presented in HEV segments, conferring focussed subset recruitment to appropriate, well-defined microenvironments for B and T cell localization within PPs. Thus, our findings demonstrate a fundamental difference in the molecular requirements for T and B cell homing, and reveal a previously unsuspected degree of vascular specialization controlling selective recruitment of circulating lymphocytes, determined not only by tissue type, but also by microdomain.
Consistent with our results, Forster and colleagues 63 have now shown that lymphocytes from CCR7-deficient mice display reduced localization to lymphoid organs, though whether the defect is at the level of HEV interaction or extravasation was not determined. They observed significant reduction of B cell homing as well, especially to LNs: in light of our findings, this may suggest that CCR7 can play a role in B cell diapedesis.
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Acknowledgments |
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This work was supported by National Institutes of Health grants GM37734 and AI37832, by National Institutes of Health Training Grant 5T32 AI07290 (to R.A.Warnock), and by a Postdoctoral Fellowship from the Arthritis Foundation (to J.J. Campbell).
Note added in proof. Vassileva et al. have now shown that there are two SLC genes in mice, and that the DDD/1 (plt) mouse mutation is a deletion of the gene responsible for SLC expression in lymphoid tissues (Vassileva, G., H. Soto, A. Zlotnik, H. Nakano, T. Kakiuchi, J.A. Hedrick, and S.A. Lira. 1999. J. Exp. Med. 190:1183–1188).
Submitted: 9 June 1999
Revised: 8 September 1999
Accepted: 12 October 1999
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Bargatze R.F. & Butcher E.C.. Rapid G protein–regulated activation event involved in lymphocyte binding to high endothelial venules, J. Exp. Med., 178, 1993, 367–372 .
Bargatze R.F., Jutila M.A. & Butcher E.C.. Distinct roles of L-selectin and integrins alpha 4 beta 7 and LFA-1 in lymphocyte homing to Peyer's patch-HEV in situthe multistep model confirmed and refined, Immunity., 3, 1995, 99–108 .[Medline]
Warnock R.A., Askari S., Butcher E.C. & von Andrian U.H.. Molecular mechanisms of lymphocyte homing to peripheral lymph nodes, J. Exp. Med., 187, 1998, 205–216 .
Butcher E.C., Williams M., Youngman K., Rott L. & Briskin M.. Lymphocyte trafficking and regional immunity, Adv. Immunol., 72, 1999, 209–253 .[Medline]
Salmi M., Tohka S., Berg E.L., Butcher E.C. & Jalkanen S.. Vascular adhesion protein 1 (VAP-1) mediates lymphocyte subtype–specific, selectin-independent recognition of vascular endothelium in human lymph nodes, J. Exp. Med., 186, 1997, 589–600 .
Kim C.H. & Broxmeyer H.E.. Chemokinessignal lamps for trafficking of T and B cells for development and effector function, J. Leukoc. Biol., 65, 1999, 6–15 .[Abstract]
Zlotnik A., Morales J. & Hedrick J.A.. Recent advances in chemokines and chemokine receptors, Crit. Rev. Immunol., 19, 1999, 1–47 .[Medline]
Campbell J.J., Hedrick J., Zlotnik A., Siani M.A., Thompson D.A. & Butcher E.C.. Chemokines and the arrest of lymphocytes rolling under flow conditions, Science., 279, 1998, 381–384 .
Bleul C.C., Fuhlbrigge R.C., Casasnovas J.M., Aiuti A. & Springer T.A.. A highly efficacious lymphocyte chemoattractant, stromal cell–derived factor 1 (SDF-1), J. Exp. Med., 184, 1996, 1101–1109 .
Gunn M.D., Tangemann K., Tam C., Cyster J.G., Rosen S.D. & Williams L.T.. A chemokine expressed in lymphoid high endothelial venules promotes the adhesion and chemotaxis of naive T lymphocytes, Proc. Natl. Acad. Sci. USA., 95, 1998, 258–263 .
Ngo V.N., Tang H.L. & Cyster J.G.. Epstein-Barr virus–induced molecule 1 ligand chemokine is expressed by dendritic cells in lymphoid tissues and strongly attracts naive T cells and activated B cells, J. Exp. Med., 188, 1998, 181–191 .
Williams M.B. & Butcher E.C.. Homing of naive and memory T lymphocyte subsets to Peyer's patches, lymph nodes, and spleen, J. Immunol., 159, 1997, 1746–1752 .[Abstract]
Willimann K., Legler D.F., Loetscher M., Roos R.S., Delgado M.B., Clark-Lewis I., Baggiolini M. & Moser B.. The chemokine SLC is expressed in T cell areas of lymph nodes and mucosal lymphoid tissues and attracts activated T cells via CCR7, Eur. J. Immunol., 28, 1998, 2025–2034 .[Medline]
Nagira M., Imai T., Yoshida R., Takagi S., Iwasaki M., Baba M., Tabira Y., Akagi J., Nomiyama H. & Yoshie O.. A lymphocyte-specific CC chemokine, secondary lymphoid tissue chemokine (SLC), is a highly efficient chemoattractant for B cells and activated T cells, Eur. J. Immunol., 28, 1998, 1516–1523 .[Medline]
Pachynski R.K., Wu S.W., Gunn M.D. & Erle D.J.. Secondary lymphoid-tissue chemokine (SLC) stimulates integrin alpha 4 beta 7-mediated adhesion of lymphocytes to mucosal addressin cell adhesion molecule-1 (MAdCAM-1) under flow, J. Immunol., 161, 1998, 952–956 .
Gallatin W.M., Weissman I.L. & Butcher E.C.. A cell-surface molecule involved in organ-specific homing of lymphocytes, Nature., 304, 1983, 30–34 .[Medline]
Streeter P.R., Berg E.L., Rouse B.T., Bargatze R.F. & Butcher E.C.. A tissue-specific endothelial cell molecule involved in lymphocyte homing, Nature., 331, 1988, 41–46 .[Medline]
Tanabe S., Lu Z., Luo Y., Quackenbush E.J., Berman M.A., Collins-Racie L.A., Mi S., Reilly C., Lo D., Jacobs K.A. & Dorf M.E.. Identification of a new mouse beta-chemokine, thymus-derived chemotactic agent 4, with activity on T lymphocytes and mesangial cells, J. Immunol., 159, 1997, 5671–5679 .[Abstract]
Sanchez-Madrid F., Simon P., Thompson S. & Springer T.A.. Mapping of antigenic and functional epitopes on the alpha- and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1, J. Exp. Med., 158, 1983, 586–602 .
Bohm S.K., Grady E.F. & Bunnett N.W.. Regulatory mechanisms that modulate signalling by G-protein-coupled receptors, Biochem. J., 322, 1997, 1–18 .[Medline]
Ye R.D. & Boulay F.. Structure and function of leukocyte chemoattractant receptors, Adv. Pharmacol., 39, 1997, 221–289 .[Medline]
Campbell J.J., Bowman E.P., Murphy K., Youngman K.R., Siani M.A., Thompson D.A., Wu L., Zlotnik A. & Butcher E.C.. 6-C-kine (SLC), a lymphocyte adhesion-triggering chemokine expressed by high endothelium, is an agonist for the MIP-3β receptor CCR7, J. Cell Biol., 141, 1998, 1053–1059 .
Nakano H., Tamura T., Yoshimoto T., Yagita H., Miyasaka M., Butcher E.C., Nariuchi H., Kakiuchi T. & Matsuzawa A.. Genetic defect in T lymphocyte-specific homing into peripheral lymph nodes, Eur. J. Immunol., 27, 1997, 215–221 .[Medline]
Gunn M.D., Kyuwa S., Tam C., Kakiuchi T., Matsuzawa A., Williams L.T. & Nakano H.. Mice lacking expression of secondary lymphoid organ chemokine have defects in lymphocyte homing and dendritic cell localization, J. Exp. Med., 189, 1999, 451–460 .
Butcher E.C.. Leukocyte-endothelial cell recognitionthree (or more) steps to specificity and diversity, Cell., 67, 1991, 1033–1036 .[Medline]
Sallusto F., Lenig D., Forster R., Lipp M. & Lanzavecchia A.. Two subsets of memory T lymphocytes with distinct homing potentials and effector functions, Nature., 401, 1999, 708–712 .[Medline]
Kim C.H., Pelus L.M., White J.R., Applebaum E., Johanson K. & Broxmeyer H.E.. CK beta-11/macrophage inflammatory protein-3 beta/EBI1-ligand chemokine is an efficacious chemoattractant for T and B cells, J. Immunol., 160, 1998, 2418–2424 .
Yoshida R., Nagira M., Imai T., Baba M., Takagi S., Tabira Y., Akagi J., Nomiyama H. & Yoshie O.. EBI1-ligand chemokine (ELC) attracts a broad spectrum of lymphocytesactivated T cells strongly up-regulate CCR7 and efficiently migrate toward ELC, Int. Immunol., 10, 1998, 901–910 .
Campbell J.J., Haraldsen G., Pan J., Rottman J., Qin S., Ponath P., Andrew D.P., Warnke R., Ruffing N. & Kassam N.. The chemokine receptor CCR4 in vascular recognition by cutaneous but not intestinal memory T cells, Nature., 400, 1999, 776–780 .[Medline]
Zabel B.A., Agace W.W., Campbell J.J., Heath H.M., Parent D., Roberts A.I., Ebert E.C., Kassam N., Qin S. & Zovko M.. Human G protein–coupled receptor GPR-9-6/CC chemokine receptor 9 is selectively expressed on intestinal homing T lymphocytes, mucosal lymphocytes, and thymocytes and is required for thymus-expressed chemokine–mediated chemotaxis, J. Exp. Med., 190, 1999, 1241–1256 .
Yoshida R., Nagira M., Kitaura M., Imagawa N., Imai T. & Yoshie O.. Secondary lymphoid-tissue chemokine is a functional ligand for the CC chemokine receptor CCR7, J. Biol. Chem., 273, 1998, 7118–7122 .
Yoshida R., Imai T., Hieshima K., Kusuda J., Baba M., Kitaura M., Nishimura M., Kakizaki M., Nomiyama H. & Yoshie O.. Molecular cloning of a novel human CC chemokine EBI1-ligand chemokine that is a specific functional ligand for EBI1, CCR7, J. Biol. Chem., 272, 1997, 13803–13809 .
Middleton J., Neil S., Wintle J., Clark-Lewis I., Moore H., Lam C., Auer M., Hub E. & Rot A.. Transcytosis and surface presentation of IL-8 by venular endothelial cells, Cell., 91, 1997, 385–395 .[Medline]
Gopalan P.K., Smith C.W., Lu H., Berg E.L., McIntire L.V. & Simon S.I.. Neutrophil CD18-dependent arrest on intercellular adhesion molecule 1 (ICAM-1) in shear flow can be activated through L-selectin, J. Immunol., 158, 1997, 367–375 .[Abstract]
Kunkel E.J., Ramos C.L., Steeber D.A., Muller W., Wagner N., Tedder T.F. & Ley K.. The roles of L-selectin, beta 7 integrins, and P-selectin in leukocyte rolling and adhesion in high endothelial venules of Peyer's patches, J. Immunol., 161, 1998, 2449–2456 .
Laudanna C., Constantin G., Baron P., Scarpini E., Scarlato G., Cabrini G., Dechecchi C., Rossi F., Cassatella M.A. & Berton G.. Sulfatides trigger increase of cytosolic free calcium and enhanced expression of tumor necrosis factor-alpha and interleukin-8 mRNA in human neutrophils. Evidence for a role of L-selectin as a signaling molecule, J. Biol. Chem., 269, 1994, 4021–4026 .
Stein J.V., Rot A., Luo Y., Narasimhaswamy M., Nakano H., Gunn M.D., Matsuzawa A., Quackenbush E.J., Dorf M.E. & von Andrian U.H.. The CC chemokine thymus-derived chemotactic agent 4 (TAC-4, secondary lymphoid tissue chemokine 6Ckine, Exodus-2) triggers lymphocyte function–associated antigen 1–arrest of rolling T lymphocytes in peripheral node high endothelial venules, J. Exp. Med., 191, 2000, 61–75 .
Weissman I.L., Gutman G.A. & Friedberg S.H.. Tissue localization of lymphoid cells, Ser. Haematol., 7, 1974, 482–504 .[Medline]
Goodnow C.C. & Cyster J.G.. Lymphocyte homingthe scent of a follicle, Curr. Biol., 7, 1997, R219–R222 .[Medline]
Gutman G.A. & Weissman I.L.. Homing properties of thymus-independent follicular lymphocytes, Transplantation., 16, 1973, 621–629 .[Medline]
Stevens S.K., Weissman I.L. & Butcher E.C.. Differences in the migration of B and T lymphocytesorgan-selective localization in vivo and the role of lymphocyte-endothelial cell recognition, J. Immunol., 128, 1982, 844–851 .[Abstract]
Pals S.T., Kraal G., Horst E., de Groot A., Scheper R.J. & Meijer C.J.. Human lymphocyte-high endothelial venule interactionorgan-selective binding of T and B lymphocyte populations to high endothelium, J. Immunol., 137, 1986, 760–763 .[Abstract]
Pellas T.C. & Weiss L.. Migration pathways of recirculating murine B cells and CD4+ and CD8+ T lymphocytes, Am. J. Anat., 187, 1990, 355–373 .[Medline]
Miura S., Tsuzuki Y., Fukumura D., Serizawa H., Suematsu M., Kurose I., Imaeda H., Kimura H., Nagata H. & Tsuchiya M.. Intravital demonstration of sequential migration process of lymphocyte subpopulations in rat Peyer's patches, Gastroenterology., 109, 1995, 1113–1123 .[Medline]
Andrew D.P., Rott L.S., Kilshaw P.J. & Butcher E.C.. Distribution of alpha 4 beta 7 and alpha E beta 7 integrins on thymocytes, intestinal epithelial lymphocytes and peripheral lymphocytes, Eur. J. Immunol., 26, 1996, 897–905 .[Medline]
Tang M.L., Steeber D.A., Zhang X.Q. & Tedder T.F.. Intrinsic differences in L-selectin expression levels affect T and B lymphocyte subset-specific recirculation pathways, J. Immunol., 160, 1998, 5113–5121 .
Yamaguchi K. & Schoefl G.I.. Blood vessels of the Peyer's patch in the mouseI. Topographic studies, Anat. Rec., 206, 1983, 391–401 .[Medline]
Butcher E.C. & Picker L.J.. Lymphocyte homing and homeostasis, Science., 272, 1996, 60–66 .[Abstract]
Mebius R.E., Breve J., Duijvestijn A.M. & Kraal G.. The function of high endothelial venules in mouse lymph nodes stimulated by oxazolone, Immunology., 71, 1990, 423–427 .[Medline]
van Halteren A.G., Mebius R.E. & Kraal G.. Vascular addressin expression in Peyer's patchesan in vivo study of site-associated regulation, Adv. Exp. Med. Biol., 355, 1994, 125–130 .[Medline]
Mebius R.E., Streeter P.R., Michie S., Butcher E.C. & Weissman I.L.. A developmental switch in lymphocyte homing receptor and endothelial vascular addressin expression regulates lymphocyte homing and permits CD4+ CD3– cells to colonize lymph nodes, Proc. Natl. Acad. Sci. USA., 93, 1996, 11019–11024 .
Kraal G. & Mebius R.E.. High endothelial venuleslymphocyte traffic control and controlled traffic, Adv. Immunol., 65, 1997, 347–395 .[Medline]
Cuff C.A., Schwartz J., Bergman C.M., Russell K.S., Bender J.R. & Ruddle N.H.. Lymphotoxin alpha3 induces chemokines and adhesion moleculesinsight into the role of LT alpha in inflammation and lymphoid organ development, J. Immunol., 161, 1998, 6853–6860 .
Connor E.M., Eppihimer M.J., Morise Z., Granger D.N. & Grisham M.B.. Expression of mucosal addressin cell adhesion molecule-1 (MAdCAM-1) in acute and chronic inflammation, J. Leukoc. Biol., 65, 1999, 349–355 .[Abstract]
Ngo V.N., Korner H., Gunn M.D., Schmidt K.N., Riminton D.S., Cooper M.D., Browning J.L., Sedgwick J.D. & Cyster J.G.. Lymphotoxin /β and tumor necrosis factor are required for stromal cell expression of homing chemokines in B and T cell areas of the spleen, J. Exp. Med., 189, 1999, 403–412 .
Tanaka Y., Imai T., Baba M., Ishikawa I., Uehira M., Nomiyama H. & Yoshie O.. Selective expression of liver and activation-regulated chemokine (LARC) in intestinal epithelium in mice and humans, Eur. J. Immunol., 29, 1999, 633–642 .[Medline]
Sozzani S., Allavena P., D'Amico G., Luini W., Bianchi G., Kataura M., Imai T., Yoshie O., Bonecchi R. & Mantovani A.. Differential regulation of chemokine receptors during dendritic cell maturationa model for their trafficking properties, J. Immunol., 161, 1998, 1083–1086 .
Sallusto F., Schaerli P., Loetscher P., Schaniel C., Lenig D., Mackay C.R., Qin S. & Lanzavecchia A.. Rapid and coordinated switch in chemokine receptor expression during dendritic cell maturation, Eur. J. Immunol., 28, 1998, 2760–2769 .[Medline]
Yanagihara S., Komura E., Nagafune J., Watarai H. & Yamaguchi Y.. EBI1/CCR7 is a new member of dendritic cell chemokine receptor that is up-regulated upon maturation, J. Immunol., 161, 1998, 3096–3102 .
Saeki H., Moore A.M., Brown M.J. & Hwang S.T.. Cutting edgesecondary lymphoid-tissue chemokine (SLC) and CC chemokine receptor 7 (CCR7) participate in the emigration pathway of mature dendritic cells from the skin to regional lymph nodes, J. Immunol., 162, 1999, 2472–2475 .
Kellermann S.A., Hudak S., Oldham E.R., Liu Y.J. & McEvoy L.M.. The CC chemokine receptor-7 ligands 6Ckine and macrophage inflammatory protein-3beta are potent chemoattractants for in vitro- and in vivo-derived dendritic cells, J. Immunol., 162, 1999, 3859–3864 .
Nagira M., Imai T., Hieshima K., Kusuda J., Ridanpaa M., Takagi S., Nishimura M., Kakizaki M., Nomiyama H. & Yoshie O.. Molecular cloning of a novel human CC chemokine secondary lymphoid-tissue chemokine that is a potent chemoattractant for lymphocytes and mapped to chromosome 9p13, J. Biol. Chem., 272, 1997, 19518–19524 .
Hedrick J.A. & Zlotnik A.. Identification and characterization of a novel beta chemokine containing six conserved cysteines, J. Immunol., 159, 1997, 1589–1593 .[Abstract]
Forster R., Schubel A., Breitfeld D., Kremmer E., Renner-Muller I., Wolf E. & Lipp M.. CCR7 coordinates the primary immune response by establishing functional microenvironments in secondary lymphoid organs, Cell., 99, 1999, 23–33.[Medline]
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