© The Rockefeller University Press, 0022-1007/1999/9/881/ $5.00
The Journal of Experimental Medicine, Volume 190, Number 6, September 20, 1999 881-884
Development of Cd8
/ and Cd8/β T Cells in Major Histocompatibility Complex Class I–Deficient Mice
Gobardhan Dasa and
Charles A. Janeway, Jr.a
a Howard Hughes Medical Institute and Yale University Medical School, New Haven, Connecticut 06510
LH-416, Howard Hughes Medical Institute and Yale University School of Medicine, 310 Cedar St., New Haven, CT 06510.203-737-1765203-785-2793
charles.janeway{at}yale.edu
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Abstract
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Peripheral CD8+ T cells mainly use CD8
/β, and their development is mainly dependent on the major histocompatibility complex (MHC) class I proteins Kb and Db in H-2b mice. In this report, we have shown that the development of CD8/β TCR-/β cells in lymphoid organs as well as in intestinal intraepithelial lymphocytes (iIELs) is dependent on the MHC class I Kb and Db proteins. In contrast, TCR-/β CD8/ cells are found mainly in iIELs, and their numbers are unaffected in KbDb double knockout mice. Most of the TCR-
/
cells in the iIELs also bear CD8/, and they are also unaffected in KbDb –/– mice. In β2-microglobulin (β2m)-deficient mice, all of the TCR-/β CD8/ and CD8/β T cells disappear, but TCR-/ cells are unaffected by the absence of β2m.
Key Words: CD8/ T cells • CD8/β T cells • major histocompatibility complex class I • KbDb-deficient mice • β2m-deficient mice
© 1999 The Rockefeller University Press
Tcells that are CD8+ recognize antigens as peptides of 8–10 amino acids bound to MHC class I molecules. Deletion of either the Kb and Db genes, the MHC class I–associated molecule β2-microglobulin (β2m), or the transporters associated with antigen processing (TAP) molecules severely reduces the numbers of CD8+ T cells in peripheral lymphoid organs 1234. In the periphery, CD8+ T cells usually express CD8/β, which serves as a coreceptor for the MHC class I molecules during antigen recognition. By contrast, the majority of the CD8+ T cells residing in gut epithelia express CD8/ 567891011. Intestinal intraepithelial lymphocytes (iIELs) are composed of roughly equal numbers of TCR-/β and TCR-/ cells. Previous studies revealed that development of TCR-/β cells among iIELs is β2m and TAP dependent, whereas / T cells are dependent on neither β2m nor TAP 1213. In this report, we examined the requirement of classical MHC class I Kb and Db molecules in the development of CD8/ and CD8/β T cells. Our results demonstrate that CD8+ T cells in peripheral lymphoid organs like spleen and lymph node primarily bear CD8/β, whereas CD8+ T cells in iIELs mainly express CD8/. In KbDb double knockout mice, most of the CD8/β-bearing TCR-/β cells disappear in the periphery as well as in iIELs. CD8/-bearing T cells are found mainly in iIELs, suggesting that their development is independent of KbDb antigens. In B6-β2m knockout mice, CD8+ TCR-/β cells disappear completely, but / T cells are unaffected even though some of them also bear a CD8 coreceptor. This suggests that the development of CD8/β TCR-/β cells is mainly dependent on Kb and Db proteins, whereas CD8/ TCR-/β cells are dependent on β2m-associated MHC class Ib molecules and CD8/ TCR-/ cells develop independently of MHC class I molecules altogether.
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Materials and Methods
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Mice and Antibodies.
C57BL/6 (B6) and B6-β2m–deficient mice were purchased from The Jackson Laboratory. KbDb double knockout mice were a gift from Dr. Hidde Ploegh (Dept. of Pathology, Harvard University School of Medicine, Boston, MA). Anti–TCR-/β (H-57), anti–TCR-/ (GL-3), and anti-CD8β (Ly-3.2) antibodies were purchased from PharMingen. Anti-CD8 (53-6.7) was purchased from Sigma Chemical Co.
Isolation of Lymphocytes.
For the isolation of lymphocytes from spleen and lymph node, spleens and lymph nodes were macerated individually using frosted glass slides. The resulting suspension was centrifuged on a Ficoll gradient at 900 g for 10 min. Cells at the interface were collected, and T cells were enriched by passing through a nylon wool column as described previously 14. iIELs were prepared as described previously 15. In brief, small intestines were harvested and washed by passing through PBS. Mesentery and Peyer's patches were carefully removed. The intestines were cut longitudinally and then in 
0.5-cm pieces. Intestinal pieces were agitated in 50 ml of extraction buffer (PBS, 3% FCS, and 10 mM EDTA) for 30 min at 37°C. This slurry was centrifuged at 2 g for 2 min to remove the aggregates. The cell suspension was layered on a discontinuous Percoll (Amersham Pharmacia Biotech) gradient. This gradient was then centrifuged at 900 g for 20 min. Cells at the interface of the 40/70% layer were collected and washed in staining buffer. Lymphocytes from the liver were prepared as described previously 16. In brief, livers were macerated using stainless steel mesh and suspended in PBS, 3% FCS. They were loaded on a discontinuous Percoll gradient and centrifuged at 900 g for 20 min. Cells were collected from the 40/70% interface, washed, and used for further experiments.
FACS® Staining and Analysis.
Cells were suspended in staining buffer (PBS, 3% FCS, 0.01% sodium azide) at a concentration of 107 cells/ml. 100 µl of the suspension was incubated with directly conjugated antibodies for 30 min on ice. Cells were washed twice with staining buffer and fixed with 1% paraformaldehyde. Fluorescence intensities were measured with a FACScanTM (Becton Dickinson).
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Results and Discussion
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For positive selection, CD8+ T cells require MHC class I proteins, and in this process the CD8 molecules serve as coreceptor 17. In peripheral CD8+ T cells, the CD8 molecule is expressed as a membrane-bound heterodimeric protein consisting of and β chains 18. However, certain CD8+ T cells express an alternate CD8/ homodimer. Thus, we examined the organ distribution of these unusual T cells. We found that most of the CD8+ T cells in spleen, lymph node, thymus, and liver express CD8/β, and they all consistently bear TCR-/β. By contrast, in iIELs most of the T cells express CD8, but only a small portion of the T cells express CD8β as well (Fig. 1 a). In spleen, lymph node, thymus, and liver, only a very small fraction of CD8+ TCR-/ cells were found. In contrast, in iIELs a large portion of the CD8/1 T cells were found to also be TCR-/+ (Fig. 1 b). In three-color staining, gating on TCR-/β revealed that in spleen, lymph node, thymus, and liver, almost all of the CD8-bearing T cells express CD8β, but in iIELs a large number of TCR-/β cells express only CD8. Among these, only a fraction (9–11%) are CD8β+ (Fig. 1 c). On further analysis of the iIELs, it was found that among iIELs, TCR-/β and TCR-/ cells are present at almost equal numbers, and a majority of the cells express the CD8 cell surface molecule (Fig. 2 a). Gating on TCR-/ cells among the iIELs revealed that most such cells express CD8 and none express CD8β (Fig. 2 b).
β2m-associated MHC class I proteins are present on the cell surface at 105–106 molecules per cell and are divided into two distinct categories. The classical MHC class I proteins are derived from the genes for K, D, and L, which in B6 mice are called Kb and Db (there is no L molecule in the H-2b haplotype). The nonclassical MHC class I proteins are called class Ib molecules, are the products of Qa, TL, and M regions, and are also coded by the non-MHC genes of the CD1 locus on chromosome 1.
To evaluate the type of MHC restriction of both CD8/ and CD8/β T cells, we examined the composition of these two groups of cells in KbDb double knockout mice. It was found that most of the CD8/β TCR-/β cells disappeared in spleen, lymph node, and liver as well as in iIELs (Fig. 3 a). In iIELs, numbers of CD81 cells were not affected. In the thymus, there were normal numbers of CD4+CD8+ double positive (DP) cells in KbDb double knockout mice. These are presumed to be of the TCR-/β lineage. However, there were virtually no CD4–CD8+ single positive (SP) cells (Fig. 3 c), and all of the DP cells were found to use CD8/β (Fig. 3 b). Therefore, these DP cells in the thymus are uncommitted immature thymocytes that had not undergone intrathymic selection. Thus, CD8/1 T cells are mostly found in iIELs. This clearly indicates the potential for recognition of nonclassical MHC class Ib molecules by T cells bearing a TCR-/β and the CD8/ homodimer.
To examine whether this group of CD8/ T cells required β2m-associated MHC class I or class Ib molecules, we analyzed the composition of CD8/ cells in β2m–/– mice. It was found that CD8+ T cells are absent in spleen, lymph node, and liver. However, substantial numbers of CD8+ T cells were present in thymus and iIELs (Fig. 4 a). In thymus, all of the CD8+ T cells bear CD4 as well. No CD8 SP (CD4–CD8+) cells were found in these mice. A careful analysis revealed that all CD8+ T cells in iIELs possess a / TCR (Fig. 4 b). Thus, the development of the vast majority of the TCR-/β CD8/β cells requires contact with Kb or Db molecules. By contrast, CD8/ TCR-/β cells depend on β2m-associated nonclassical MHC class Ib molecules. The development of CD8+ TCR-/ cells in iIELs either does not require MHC class I for their positive selection or they are restricted to β2m-independent MHC class I molecules. The existence of β2m-independent MHC class I molecules is as yet an open question. Recently, a group of stress-induced β2m-independent MHC class I molecules was reported in humans, but the homologous genes are not found in mice 19. Thus, the diversity of a β2m-independent MHC class I molecule in the gut is a worthy goal for future research on the development of CD8+ / T cells.
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Acknowledgments
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We gratefully acknowledge Mr. Grigoroy Losyev for timely supplying antibodies, Ms. Jennifer Boucher-Reid for excellent secretarial assistance, and Mr. Scott Sheridan for excellent technical assistance.
This work was supported by grants from the Howard Hughes Medical Institute.
Submitted: 1 June 1999
Revised: 16 July 1999
Accepted: 16 July 1999
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Abstract
Materials and Methods
