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J. Exp. Med., Volume 189, Number 9, May 3, 1999 1489-1496

Induction of Fas Ligand Expression by HIV Involves the Interaction of Nef with the T Cell Receptor zeta  Chain

By Xiao-Ning Xu,* Bernd Laffert,Dagger Gavin R. Screaton,* Michael Kraft,Dagger Dietlinde Wolf,Dagger Waldemar Kolanus,§ Juthathip Mongkolsapay,* Andrew J. McMichael,* and Andreas S. BaurDagger

From the * Medical Research Council Human Immunology Unit, Institute of Molecular Medicine, John Radcliffe Hospital, Oxford OX3 9DS, United Kingdom; the Dagger  Institute of Clinical and Molecular Virology, University of Erlangen/Nürnberg, 91054 Erlangen, Germany; and § Genzentrum, Ludwig-Maximilians-Universität München, 81377 München, Germany

    Abstract
Top
Abstract
Introduction
Materials and Methods
Results
Discussion
References

During HIV/SIV infection, there is widespread programmed cell death in infected and, perhaps more importantly, uninfected cells. Much of this apoptosis is mediated by Fas-Fas ligand (FasL) interactions. Previously we demonstrated in macaques that induction of FasL expression and apoptotic cell death of both CD4+ and CD8+ T cells by SIV is dependent on a functional nef gene. However, the molecular mechanism whereby HIV-1 induces the expression of FasL remained poorly understood. Here we report a direct association of HIV-1 Nef with the zeta  chain of the T cell receptor (TCR) complex and the requirement of both proteins for HIV-mediated upregulation of FasL. Expression of FasL through Nef depended upon the integrity of the immunoreceptor tyrosine-based activation motifs (ITAMs) of the TCR zeta  chain. Conformation for the importance of zeta  for Nef-mediated signaling in T cells came from an independent finding. A single ITAM motif of zeta  but not CD3epsilon was both required and sufficient to promote activation and binding of the Nef-associated kinase (NAK/p62). Our data imply that Nef can form a signaling complex with the TCR, which bypasses the requirement of antigen to initiate T cell activation and subsequently upregulation of FasL expression. Thus, our study may provide critical insights into the molecular mechanism whereby the HIV-1 accessory protein Nef contributes to the pathogenesis of HIV.

Key words: Jurkat;  immunoreceptor tyrosine-based activation motif;  Nef-associated kinase;  activation-induced cell death;  apoptosis
    Introduction
Top
Abstract
Introduction
Materials and Methods
Results
Discussion
References

In HIV/simian immunodeficiency virus (SIV)1 infection, the nef gene plays a key role in viral replication and progression of disease. This is based on studies in macaques and humans, who remain asymptomatic or long-term nonprogressing when infected with an SIV mutant lacking a nef gene or HIV with multiple nef deletions, respectively (1). More recently, a study using a transgenic mouse model has demonstrated that Nef harbors a major determinant for HIV-induced pathogenicity (4). Despite the considerable importance of Nef for HIV/SIV pathogenesis, its function at the molecular level is poorly understood. At least three in vitro effects of Nef have been described. Nef downregulates the surface receptors CD4 and MHC I (5, 6), increases viral infectivity (7), and stimulates T cell signaling pathways (8).

The major consequence of HIV infection is the depletion of T cells leading to immunoparesis characteristic of AIDS. This is likely due to the widespread programmed cell death (apoptosis) induced by HIV (11). Several different apoptotic pathways have been proposed in HIV infection, including Fas/FasL (14), TNF/TNFR (15), and an interaction between TNF-related apoptosis-inducing ligand (TRAIL) and its receptors (16). In HIV-infected patients, there is upregulation of both Fas and FasL as well as an increased susceptibility of CD4+ and CD8+ cells to Fas-mediated killing (17). Recently, we have demonstrated in macaques that induction of FasL expression and apoptotic cell death of both CD4+ and CD8+ T cells by SIV is dependent on a functional nef gene (22). However, a molecular mechanism integrating this observation into other documented effects of Nef is lacking.

The concept of Nef interfering with early events emanating from the TCR could explain its dual effects on T cell activation and FasL expression, since both functions are regulated by the TCR complex (23). Here we report that HIV-mediated upregulation of FasL in T cells is dependent on the association of Nef with the TCR zeta  chain. By demonstrating that Nef directly targets the TCR of the infected cell, we provide novel insight into the molecular function of Nef in HIV infection.

    Materials and Methods
Top
Abstract
Introduction
Materials and Methods
Results
Discussion
References

Cell Lines and Antibodies.

Generation of Jurkat cell lines constitutively expressing CD8 tag or CD8-Nef chimeras was described recently (8, 26). Jurkat, J.CaM.1 (Lck-), J.45.01 (CD45-), and J.RT3-T3.5 (TCR-) cells were provided by Arthur Weiss (University of California, San Francisco, CA [27]). J.RT3-T3.5 cells expressing various CD16zeta constructs or coexpressing CD8-Nef were generated by electroporation using puromycin and neomycin for selection. Stable clones were enriched for protein expression by magnetic anti-CD16/anti-CD8 beads. The mAbs against the AU-5/ AU-1 epitope and FasL (NOK-1) were purchased from Hiss Diagnostics and PharMingen.

Plasmid Constructions.

Generation of the CD8-Nef (SF2) and CD16zeta /epsilon chimeras as well as COOH-terminal-tagged Nef (AU-1) was described previously (8, 26, 28). Fusion proteins between CD16 and individual zeta  ITAMs (ITAM 1, amino acids [aa] 1-70; ITAM 2, aa 70-110; ITAM 3, aa 110-141) were generated as described previously (28). The mutations in CD16zeta as well as in Nef/CN.94 were generated by a two-step PCR procedure and cloned into the pRcCMV expression vector (Invitrogen). In CD16zeta mu, the tyrosine residues in three ITAM motifs (two tyrosine residues in each ITAM) were mutated to alanines. In CN.94PXmu/Nef.PXmu, the FPVR motif of Nef (aa 72-75) was mutated to VRIT. Construction of the proviral clone NL4-3, containing the SF2 nef gene (NL4-3.SF2Nef), as well as the Nef-negative construct (NL4-3Delta Nef), was described previously (26). For the generation of recombinant baculoviruses, the "bac to bac" system was used (Bio-Rad Laboratories).

Protein Expression Assays.

Transfections into 293T cells, metabolic labeling with 35S-Translabel, immunoprecipitation, Western blot, and in vitro kinase assays were performed as described previously (8, 26). The immunoprecipitates were washed three times (wash buffer: 1% NP-40, 450 mM NaCl, 50 mM Tris-HCl [pH 8], 1 mM EDTA). To show an interaction between Nef and zeta , extraction and washing buffers contained 1% Brij instead of 1% NP-40. FasL promoter activity was tested as described previously (29) by cotransfection of pFasL-Luc (provided by Xiangdong Liu, Department of Virus and Cancer, Aarhus, Denmark) with CD8-Nef constructs or pCTax as positive control (provided by Ralph Grassman, Institute of Virology, Erlangen, Germany). All transfections were performed in duplicate by mixing 6 µl of liposome reagent (DMRIE-C; GIBCO BRL) and 2 µg of each plasmid for 2 × 106 Jurkat or Jurkat mutant cells.

In Vitro HIV Infection of Jurkat or Jurkat Mutant Cell Lines.

Cells (5 × 106) were superinfected with 1 ml of HIV IIIB (1.6 × 104 cpm/ml, reverse transcriptase [RT] activity), NL4-3.SF2.Nef (2.4 × 104 cpm/ml, RT activity), or NL4-3Delta Nef (2.5 × 104 cpm/ml, RT activity) for 2 h. After infection, cells were washed and adjusted to a concentration of 106/ml and incubated for an additional 48 h. Cell culture supernatants were collected on day 5 for analysis of p24 by ELISA or RT activity by Quan-T-RT kit (Amersham Pharmacia Biotech).

Analysis of FasL Expression by Flow Cytometry and Immunoprecipitation.

To assess cell surface FasL expression on HIV-infected cells or transiently transfected Jurkat TAg cells, the metalloprotease inhibitor BB2116 (British Biotech [30]) was added to the medium 4-6 h before the assay to enhance cell surface FasL expression. In brief, cells were stained with 20 µl of biotin-conjugated anti-human FasL mAb (NOK-1; PharMingen) followed by 5 µl of PE-conjugated streptavidin (Sigma). Labeled cells were analyzed on a FACScanTM (Becton Dickinson). Isotype-specific mAbs of irrelevant specificity were used as negative controls (Dako Diagnostics). To assess expression of whole FasL protein, 35S-labeled cells (5 × 106) were immunopreciptatied for FasL using anti-FasL-specific mAb (NOK-1) as described previously (30).

    Results
Top
Abstract
Introduction
Materials and Methods
Results
Discussion
References
Requirement of the TCR zeta  Chain for Binding of Nef-associated Kinase (p62) to Nef.

As shown previously, Nef associates with a serine kinase, termed p62 or Nef-associated kinase (NAK [31]). The Nef-NAK interaction is complex: Nef stimulates the phosphorylation/activation of NAK, and it is only in this activated form that NAK can bind Nef (32). This suggests that Nef must act upstream of NAK to promote NAK activation. Our previous results showing that Nef interfered with early signals emanating from the TCR suggested it may interact with a component of the TCR signaling complex. This prompted us to study Nef-mediated NAK/p62 activation in cell lines with TCR signaling defects. CD8-Nef chimeras (CD8-Nef), containing the extracellular domain of CD8alpha fused to Nef, were stably transfected into wild-type Jurkat and a variety of Jurkat mutant cell lines lacking either Lck (J.CaM.1), CD45 (CD45-), or the entire TCR signaling complex (RT3.T3.5). Expression of CD8-Nef in these cell lines was verified by metabolic labeling and immunoprecipitation (Fig. 1 B). The Nef chimeras from these transfectants were immunoprecipitated and subjected to an in vitro kinase assay. NAK/p62 association was observed in all cell lines except the TCR- cells (Fig. 1 A). The latter result was confirmed in a second, independently transfected cell clone (data not shown).


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  		Fig. 1.   Requirement of the TCR zeta  chain for binding of NAK/p62 to Nef. (A) In vitro kinase assay after immunoprecipitation (IP) of CD8-Nef chimeras using the CD8 tag from stably transfected wild-type and mutant Jurkat cell lines lacking Lck, TCR, or CD45. Lane 1, control (Cont.) Jurkat transfected with the CD8 tag. (B) Control immunoprecipitation showing expression of 35S-labeled CD8-Nef. (C) In vitro kinase assay after immunoprecipitation of CD8-Nef to study NAK/p62 association/phosphorylation in wild-type (Cont., lane 1) and TCR- Jurkat cell lines (lane 2), and after coexpression (stable transfection) of CD8-Nef with either CD16epsilon , CD16zeta 2 (ITAM 2), CD16zeta 3 (ITAM 3), or CD16zeta mu (mutation of all three ITAMs) in the TCR- cell line (lanes 3-6). (D) Control immunoprecipitation of 35S-labeled CD8-Nef from cell lines shown in C. (E) In vitro kinase assay after immunoprecipitation of AU-1-tagged Nef from transiently transfected 293T cells. Lane 1, transfection with Nef alone; lanes 2-7, cotransfection with increasing amounts (0.5 and 1 µg) of CD16epsilon , CD16zeta , or CD16zeta mu. (F) The nitrocellulose filter shown in E was blotted (WB) with an anti-Nef (AU-1) antibody to verify comparable Nef expression.

Next we asked whether NAK binding could be restored in cells lacking the TCR complex by stable transfection with TCR-zeta or CD3epsilon fused to the extracellular domain of CD16 (CD16zeta and CD16epsilon ). These TCR subcomponents contain signaling motifs (immunoreceptor tyrosine-based T cell activation motifs [ITAMs]), which are required and sufficient for T cell activation (28, 33). After obtaining single cell clones, expression of the chimeras was verified by metabolic protein labeling and FACS® analysis (data not shown). In several attempts, we were unable to coexpress CD8-Nef (CN) with CD16zeta in TCR- cells. Cell clones that were obtained either showed no detectable CN or CD16 expression or died rapidly. The effect resembled activation-induced cell death (AICD) by Nef as reported previously (8). Coexpression of CN and CD16epsilon was achieved; however, the obtained cell clones had a low CN as well as CD16 surface expression (Fig. 1 D, lane 3). Therefore, we constructed zeta  chimeras containing the three individual zeta  ITAMs in isolation (CD16zeta 1, 2, or 3; see Materials and Methods for details). In a seperate construct, the tyrosine residues in all three YXXL motifs of CD16zeta were mutated to alanines (CD16zeta mu). We failed to coexpress the first zeta  ITAM with Nef. However, NAK/p62 binding to Nef was reestablished in the TCR- cells by coexpression with the second or third ITAM of zeta  (Fig. 1 C, lanes 4 and 5). In these latter cell lines, expression of CD16zeta 1 and 2 as well as CD8-Nef decreased significantly over time (data not shown), indicating that coexpression of both proteins was not favorable. The difficulties regarding the coexpression of the individual zeta  ITAMs with CD8-Nef may be explained by studies published by Combadiere et al. (34) showing that in particular the first zeta  ITAM but much less the second and third are capable of inducing apoptosis when activated. The signaling-defective zeta  chain (CD16zeta mu) expressed well, but NAK binding to Nef was greatly reduced (lane 6). No NAK/p62 binding was observed by coexpression of CD3epsilon (lane 3). Since NAK binding to Nef was not completely negative with CD16zeta mu, the Nef-zeta complex may recruit additional signaling molecules to the plasma membrane which are important for NAK activation. Assuming that the effects of the first zeta  ITAM would be similar to ITAM 2 and 3, it appeared that at least one functional ITAM of the CD3 zeta  chain was required for binding of p62/NAK to Nef.

The functional link between Nef, zeta , and NAK was confirmed by transient transfection assays in a heterologous system. As shown in Fig. 1 E, cotransfection of CD16zeta (lanes 4 and 5) but not CD16epsilon (lanes 2 and 3) significantly increased binding of p62/NAK to Nef. A minimal increase was seen after cotransfection of CD16zeta mu (lanes 6 and 7), which paralleled the small effect seen in Fig. 1 C, lane 6. Thus, no other T cell-specific components except the functional ITAM(s) from the TCR zeta  chain, were required for NAK activation and NAK/Nef association in 293T cells.

Direct Association of Nef with the TCR zeta  Chain.

Full-length CD8-Nef when expressed at the cell membrane promotes AICD. Upon stable transfection, cell clones are preferentially selected in which Nef is predominantly expressed in the cytoplasm, where it does not exert such a detrimental effect on cell survival. In contrast, NH2-terminal fragments of Nef are expressed at high levels at the plasma membrane where TCR-zeta is located (8). These NH2-terminal fragments can recruit a complex of proteins to form the NH2-terminal kinase complex, which binds between amino acids 20 and 35, and may contribute to T cell activation (26). The NH2 terminus also has a conserved domain containing a proline-rich motif (PxxP, aa 73-82) known to associate with SH3 domains of tyrosine kinases (35). We reasoned that these domains/motifs could interact with and bind TCR-zeta . To prove a direct interaction, coimmunoprecipitation experiments were performed using stable cell lines with different CD8-Nef chimeras (Fig. 2, A and B). The only interaction was seen with a construct expressing the NH2-terminal 94 amino acids of Nef containing the PxxP motif (lane 4). Underscoring the importance of the PxxP motif for zeta  binding, we found that point mutations in the PxxP motif of the 94-amino acid Nef construct (CN.94.PXmu) almost completely abolished zeta  binding (lane 5).


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  		Fig. 2.   Association of membrane-associated Nef with the TCR zeta  chain. (A) Anti-Nef (CD8) immunoprecipitation, then anti-zeta Western blot (WB). (B) Anti-zeta immunoprecipitation, then anti-Nef Western blot from wild-type Jurkat cells stably transfected with CD8 tag (Cont.), and CD8-Nef chimeras containing full-length Nef expressed in the cytoplasm (CD8.Nef.cyt.), the NH2-terminal 49 amino acids of Nef (CD8-Nef.49), the NH2-terminal 94 amino acids (CD8-Nef.94), and CD8-Nef.94PXmu in which the PxxP motif has been mutated. (C) Control immunoprecipitation of CD8.Nef chimeras from 35S-labeled cells to show a comparable protein expression (*). (D) Nef-zeta association after baculovirus coinfection of Hi5 cells. Control anti-Nef Western blot (WB) after anti-Nef (AU-1) immunoprecipitation from Hi5 cells infected with wild-type Nef or Nef with a mutated PxxP motif (lanes 1 and 2). Immunoprecipitated Nef from Hi5 cells ran, in addition to monomers (Nef-m), as dimers (Nef-d) or higher order multimers (arrows), which may be important for Nef function. Hi5 cells were then coinfected with wild-type Nef and zeta  fused to CD16 (CD16zeta ; lanes 3-6). CD16zeta was immunoprecipitated using an AU-5 tag, and the immunoprecipitates were blotted for Nef (AU-1). Two immunoprecipitations were performed in the presence of cytoplasmic lysates from Jurkat (zeta -positive; lane 4) or TCR-zeta -negative cells (lane 5). The positions of the antibody heavy (*) and light (**) chains are indicated. (E) Control Western blot. The nitrocellulose filter shown in D was stripped and blotted with an antibody recognizing the CD16zeta construct (AU-5).

Further evidence for an interaction between Nef and zeta  was obtained by coimmunoprecipitation of Nef and zeta  from Hi5 insect cells coinfected with recombinant baculoviruses (Fig. 2 D). CD16zeta was found to coprecipitate with Nef (Fig. 2 D, lane 3), but not with Nef.PXmu (lane 6). To confirm the specificity of the interaction, aliquots of the anti-zeta immunoprecipitates were incubated with Jurkat (zeta -containing) or the TCR- (zeta -lacking) cytoplasmic lysates. Wild-type Jurkat competed for Nef binding (lane 4), whereas the TCR-zeta -negative cytoplasmic lysates did not (lane 5). The reduced Nef signal in lane 5 may be explained by the reduced amount of immunoprecipitated zeta  (Fig. 2 E, lane 5). Additional evidence for the interaction of both proteins was obtained by coimmunoprecipitation after transient transfection into COS cells and subsequent in vitro kinase assay (data not presented).

Upregulation of FasL Expression by HIV Requires Both Intact Nef and TCR zeta  Chain.

We have previously shown that the upregulation of FasL in SIV infection requires an intact nef gene (22). In general, the level of cell surface FasL expression is quite low when analyzed by FACS® even when metalloproteinase inhibitors are used which block cleavage of FasL from the cell surface. In view of this difficulty, we used additional experimental approaches to analyze Nef-mediated FasL expression (see below). Since stimulation of TCR-zeta effectively upregulates FasL expression (34, 36), we speculated that the interaction of Nef with TCR-zeta would lead to a similar effect. First, to show that HIV-Nef is required for FasL upregulation, we infected Jurkat with wild-type HIV (NL4-3.SF2Nef) or a mutant lacking the nef gene (NL4-3Delta Nef) (Fig. 3). Little if any FasL is seen on cells infected with Nef-deleted HIV, thus confirming our previous results with SIV. The level of viral replication in Jurkat cells was comparable, as determined by RT activity (NL4-3.SF2Nef, 4.6 × 103 cpm; NL4-3Delta Nef, 5.8 × 103 cpm). Upregulation of FasL by HIV is also lost in mutant Jurkat cells lacking the TCR complex, whereas cells reconstituted with zeta  but not with the zeta  mutant restored the FasL expression upon HIV infection as determined by both immunoprecipitation (Fig. 4 A) and FACS® analysis (Fig. 4 B). Viral replication assessed by p24 assay indicated that these cell lines were comparably infected (wild-type, 3.5 ± 0.5; TCR-, 3.8 ± 1.0; CD16zeta , 3.8 ± 0.5; CD16zeta mu, 3.1 ± 0.25 ng/ml).


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  		Fig. 3.   Nef is required for FasL upregulation. Jurkat cells were infected with (A) wild-type HIV-1 (NL4-3.SF2Nef) or (B) SF2 lacking the nef gene (NL4-3Delta Nef). After 48 h, FasL expression (solid line) was assessed by flow cytometry and compared with staining with a control mAb (dashed line). The level of viral replication in Jurkat cells was comparable as determined by RT activity (NL4-3.SF2Nef, 4.6 × 103 cpm; NL4-3Delta Nef, 5.8 × 103 cpm).


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  		Fig. 4.   FasL upregulation by HIV requires the TCR zeta  chain. FasL expression was assessed 48 h after infection with HIV (IIIB strain) by immunoprecipitation using an anti-FasL mAb (A) or by flow cytometry (B). Wild-type (WT) and TCR- Jurkat are compared with the TCR- cells stably transfected with CD16zeta or CD16zeta mu (signaling-defective CD16zeta ). The level of viral replication in Jurkat cells was comparable as determined by p24 assay (wild-type, 3.5 ± 0.5; TCR-, 3.8 ± 1.0; CD16zeta , 3.8 ± 0.5; CD16zeta mu, 3.1 ± 0.25 ng/ml).

Nef Can Induce FasL Expression in the Presence of TCR-zeta .

To investigate a direct upregulation of FasL by Nef, a CD8-Nef construct not capable of binding zeta  (Nef.PXmu; see Fig. 2 B) and CD8-Nef were transiently expressed in Jurkat cells and analyzed for FasL upregulation. CD8-Nef but not the Nef mutant led to a significant cell surface expression of FasL (Fig. 5). Next, FasL upregulation was studied in Jurkat and TCR mutant cell lines using a FasL promotor/luciferase reporter construct. The latter has been shown to be stimulated in transient assays by the HTLV I Tax protein (29). Nef stimulated the FasL promotor in Jurkat and TCR- mutant cells reconstituted with the TCR zeta  chain. No effect was seen using the Nef.PXmu construct or the TCR- Jurkat cell line (Fig. 6). These assays confirmed that a functional Nef protein and the TCR zeta  chain were both required and sufficient to upregulate FasL in T cells.


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  		Fig. 5.   Induction of FasL expression by Nef or a Nef mutant (Nef.PXmu). Jurkat cells were transfected with CD8-Nef or CD8-Nef.PXmu (no binding to TCR-zeta ) construct by electroporation and kept under neomycin selection for 2 wk. Outgrowing cells were selected for CD8 surface expression by Dynabeads and analyzed for FasL expression by FACS® as described above.


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  		Fig. 6.   Upregulation of FasL by Nef requires TCR-zeta . Transient cotransfection of a FasL promotor/luciferase reporter with an empty vector (cont.; negative control), Tax (positive control), Nef, and Nef.PXmu into Jurkat (WT), TCR- Jurkat (TCR-), and TCR- cells reconstituted with CD16-zeta (TCR-/Zeta). The expression of the reporter gene was determined as described in Materials and Methods. Bars, the mean ± SD of three experiments.

    Discussion
Top
Abstract
Introduction
Materials and Methods
Results
Discussion
References

In general, the interaction between Fas and FasL plays a important role in the homeostatic regulation of normal immune responses (37). Stimulation of the TCR-CD3 complex in T cells causes upregulation of FasL and eventually leads to AICD or apoptosis (23). A key molecule in this process is the TCR zeta  chain and the three ITAMs contained therein. Cross-linking of the zeta  chain or constructs containing individual zeta  ITAMs alone were found to be sufficient to induce T cell activation and Fas-mediated apoptosis (28, 35, 36). In agreement with these findings, we have shown here that TCR-zeta as well as the functional integrity of the ITAM signaling motifs of zeta  were required for HIV-mediated upregulation of FasL. However, these findings further implied that HIV targets the TCR zeta  chain directly through a viral protein.

To date, several lines of evidence indicated that the Nef protein exerted such a role. First, Nef-mediated activation of T cells has been demonstrated in a number of reports (8- 10). Second, expression of Nef in the cytoplasm of T cells interferes with early T cell signaling events emanating from the TCR-CD3 complex, including hypophosphorylation of TCR-zeta , whereas expression of a plasma membrane- associated form of Nef causes AICD in Jurkat cells (8). Third, a very aggressive form of Nef from SIV, SIV-YE-Nef, basically functions like an ITAM domain of TCR-zeta (38). Finally, SIV-induced upregulation of FasL in T cells depends on the expression of an intact Nef protein (22), and Nef from a lethal SIV strain (smmPBj14) alone can directly cause FasL upregulation (39). Thus, it appeared very likely that Nef acted at the level of the TCR. Indeed, our study confirms this assumption by showing that Nef can directly interact with the TCR zeta  chain.

Strong evidence for the interaction of Nef with zeta  came from a second, surprising finding. In Jurkat cells lacking the TCR, binding of the Nef-associated serine kinase p62/NAK was abolished. Conversely, reconstitution of these cells with the zeta  ITAM 2 and 3 restored binding of p62/NAK with Nef. Furthermore, the integrity of the ITAM motif appeared to be important, since mutation of the zeta  ITAMs greatly reduced the effect. As shown previously, the p62/NAK kinase has to be activated in order to bind to Nef (32). These results suggest a dynamic interaction of Nef with the zeta  ITAMs, ultimately resulting in the activation of p62/NAK, which in turn binds to Nef. In view of our and other studies, it is likely that activation of p62/NAK is part of Nef-mediated stimulation of T cell signaling pathways; however, at this point it is not clear whether p62/NAK has a role in the Nef-mediated upregulation of FasL. Notably, Nef binds to p62/NAK in cells lacking a TCR (31; e.g., COS cells). In these cells, the TCR zeta  chain may be functionally replaced by other receptors, possibly containing ITAMs. This would explain why Nef has effects in cells usually not infected by HIV (40; e.g., NIH 3T3 cells).

More recently, Howe et al. showed that Nef from SIV or HIV-2 associated with the TCR zeta  chain but failed to show an interaction with HIV-1 Nef (41). Our study differs from that of Howe et al. in at least two respects. First we made constructs to target Nef to the plasma membrane where the TCR is located. Second, we have established functional consequence of the Nef-zeta interation which may have relevence to the pathogenesis of HIV interaction.

Induction of cell death by HIV could be mediated by different viral proteins. Cross-linking of CD4 by HIVgp120 in the presence of Tat protein can induce FasL expression and apoptosis of uninfected T cells (42). Additionally, interaction of HIVgp120 with chemokine receptor CXCR4 on macrophages leads to death of CD8+ T cells mediated by TNF-TNFRII interaction (15). In this study, we report an additional important mechanism of HIV-mediated apoptosis by demonstrating that Nef directly interacts with TCR-zeta and that both molecules are required for HIV-mediated upregulation of FasL. The interaction between Nef and TCR-zeta forms a signaling complex, bypassing the requirement for TCR ligation by antigen, and allowing HIV/SIV to activate T cells and upregulate FasL expression on the infected cells.

Thus, upregulation of FasL by Nef on HIV- or SIV- infected cells may, like FasL expression at sites of immune privilege and on some tumors, allow infected cells to evade the immune response. In addition, the effect of immune evasion is enhanced by Nef-mediated downregulation of surface MHC class I and CD4 expression (5, 6, 22, 43-46; see Fig. 7). Taken together, our results provide additional insights into the molecular mechanism whereby the HIV accessory protein Nef regulates T cell activity and contributes to the pathogenesis of HIV.


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  		Fig. 7.   Model describing mechanisms of immune evasion mediated by the HIV nef gene. Nef is expressed in the early viral life cycle and, after myristoylation, associates with the plasma membrane where several protein interactions take place. Nef interacts with zeta , which leads to the activation of p62/ NAK, which in turn causes the binding of p62/NAK to Nef. These events ultimately stimulate FasL expression, which may protect infected cells from CTL attack by killing Fas+ viral-specific CTLs in the process (1). Nef can also downregulate MHC class I expression and protect the infected cells against killing by CTLs (2), or CD4 expression leading to loss of CD4 T cell function (2).

    Footnotes

Address correspondence to Andreas S. Baur, Institute of Clinical and Molecular Virology, University of Erlangen/Nürnberg, Schlossgarten 4, 91054 Erlangen, Germany. Phone: 49-9131-852-6784; Fax: 49-9131-852-101; E-mail: asbaur{at}viro.med.uni-erlangen.de

Received for publication 8 February 1999.

   X.-N. Xu, B. Laffert, and G.R. Screaton contributed equally to this work.

We thank Drs. Tao Dong for help in virus infection and p24 assay, Xiangdong Liu for the pFasL-luc construct, Ralph Grassman for the pCTax construct, and Arthur Weiss for Jurkat mutant cell lines.

This study was supported in part by the Commonwealth AIDS grant (Australia), the Medical Research Council and the Wellcome Trust (UK), and the Deutsche Forschungsgemeinschaft (Germany).

Abbreviations used in this paper aa, amino acid(s); AICD, activation- induced cell death; ITAM, immunoreceptor tyrosine-based activation motif; mu, mutant; NAK, nef-associated kinase; RT, reverse transcriptase; SIV, simian immunodeficiency virus.

    References
Top
Abstract
Introduction
Materials and Methods
Results
Discussion
References

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