© The Rockefeller University Press, 0022-1007/1997/3/893/ $5.00
The Journal of Experimental Medicine, Volume 185, Number 5, March 3, 1997 893-900
Role of the Multiple T Cell Receptor (TCR)-
Chain Signaling Motifs in Selection of the T Cell Repertoire
Elizabeth W. Shores*,
Tom Tran*,
Alexander Grinberg
,
Connie L. Sommers,
Howard Shen, and
Paul E. Love
From the * Division of Hematologic Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892; and the Laboratory of Mammalian Genes and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892
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Abstract
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Immature thymocytes undergo a selection process within the thymus based on their T cell antigen receptor (TCR) specificity that results either in their maturation into functionally competent, self-MHC–restricted T cells (positive selection) or their deletion (negative selection). The outcome of thymocyte selection is thought to be controlled by signals transduced by the TCR that vary in relation to the avidity of the TCR–ligand interaction. The TCR is composed of four distinct signal transducing subunits (CD3-
, -
, -
, and 
) that contain either one (CD3-, -, -) or three (-) signaling motifs (ITAMs) within their intracytoplasmic domains. A possible function for multiple TCR ITAMs could be to amplify signals generated by the TCR during selection. To determine the importance of the multiple TCR- chain ITAMs in thymocyte selection, transgenes encoding 
/βTCRs with known specificity were bred into mice in which chains lacking one or more ITAMs had been genetically substituted for endogenous . A direct relationship was observed between the number of chain ITAMs within the TCR complex and the efficiency of both positive and negative selection. These results reveal a role for multiple TCR ITAMs in thymocyte selection and identify a function for TCR signal amplification in formation of the T cell repertoire.
Address correspondence to Elizabeth W. Shores, Division of Hematologic Products, Center for Biologics, HFM 538, Bldg. 29A, Rm 2B23, 29 Lincoln Drive, MSC 4555, Bethesda, MD 20892-4555.
During their development, thymocytes are subjected to a selection process that results in the survival of functionally competent, self-MHC–restricted cells (positive selection), and the deletion of potentially autoreactive cells (negative selection; reference 1). The fate of immature thymocytes is ultimately dictated by the specificity of their TCRs (2) and is presumably controlled by TCR-mediated signals that vary with the avidity of TCR-self ligand interactions within the thymus. The TCR complex contains multiple signal transducing subunits (CD3-, -, -, and ) that share a common functional sequence, the immunoreceptor tyrosine-based activation motif (ITAM)1 within their intracytoplasmic domains (3, 4). After TCR engagement, phosphorylation of ITAMs leads to the recruitment of SH2 domain–containing proteins (e.g., tyrosine kinases) to the TCR complex and initiation of the T cell activation cascade (4–7). The CD3 subunits each contain a single ITAM, whereas contains three ITAMs within its longer cytoplasmic tail. ITAM sequences are conserved but nonidentical, and it remains unclear whether individual motifs perform unique or similar functions (8). It has been suggested that due to its singular configuration, chain may function as the predominant TCR signaling structure and that its triplicated ITAMs may serve primarily to facilitate TCR signal amplification (9, 10).
chain is required for normal T cell development as -deficient (–/–) mice have markedly reduced numbers of both CD4+CD8+ (double positive, DP) and CD4+CD8– and CD4– CD8+ (single positive, SP) thymocytes (11–14). However, the major developmental defects in –/– mice were subsequently shown to be the result of impaired TCR surface expression rather than lack of -mediated signals per se, as transgene-encoded chain variants that lacked ITAM sequences but could still facilitate TCR surface expression (-0 ITAM), restored T cell development in –/– mice (15). These results demonstrated that chain signals were not specifically required for T cell development as signal transduction by TCR complexes that contain the CD3 subunit ITAMs, but not chain ITAMs, was sufficient for the generation of mature T cells.
A specific role for the multiple ITAM TCR structure could be in selection of the T cell repertoire during thymocyte development. Recent data support the idea that relatively low affinity TCR–ligand interactions can promote the selection of immature thymocytes, whereas higher affinity interactions are required for mature T cell activation (2, 16–20). Thus, the multiple chain ITAMs could be required to amplify TCR signals originating from the relatively weak interactions that mediate thymocyte selection. Since this question could not be easily addressed in mice with a heterogeneous population of T cells with diverse TCR specificities, we fixed the specificity of the TCRs expressed on developing thymocytes by introducing transgenes that encode productively rearranged TCR- and -β chains (/β-TCR Tg) into –/– mice (21, 22). We then bred into the /β-TCR Tg/–/– background transgenes encoding either full-length chain (3 ITAM), chains containing a single ITAM (1 ITAM), or a chain lacking all three ITAMs (- 0 ITAM). The results of these studies reveal that chain signals influence both positive and negative thymocyte selection and identify a role for the multiple ITAM TCR structure in selection of the T cell repertoire.
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Materials and Methods
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Transgenic Mice.
Transgenes encoding full-length chain (-3 ITAM Tg) and chain variants with intact extracellular and transmembrane domains, but containing only a single ITAM (either the membrane proximal ITAM or the membrane distal ITAM; [-1 ITAM Tg]), or containing no ITAMs (-0 ITAM Tg) were generated using the identical (human CD2) promoter/ enhancer cassette. The generation of –/– mice, transgenes, and genetic reconstitution of –/– mice with transgenes has been described previously (11, 15). The /β-TCR transgenes used in these experiments encoded either an MHC class I–restricted TCR specific for male antigen (H-Y; 21) or an MHC class II–restricted TCR specific for pigeon cytochrome C (AND; 22). /βTCR transgenes were introduced into the –/– background by breeding and identified by Southern blotting or PCR analysis of tail DNA. All mice used for these studies were bred and maintained in a barrier (specific pathogen-free) facility.
Proliferation Assays.
Single cell suspensions were prepared from LNs from normal (+/+) or transgene-reconstituted –/– mice. T cells were purified by incubating cells on rabbit anti–mouse Igcoated plates. Accessory cells and APCs (either for FcR-mediated antibody cross-linking or allogeneic stimulator cells) were prepared from spleen cell suspensions of C57BL/6 or BALB/c mice. APCs were depleted of T cells with anti-Thy1.2 + C' and irradiated with 3,000 rads. 105 responder T cells were combined with 5 x 105 accessory cells in flat-bottom 96-well plates in the presence or absence of the indicated stimulants. After the specified time, cells were pulsed for 8 h with 1 µCi [3H]thymidine and harvested. For mitogen stimulation, Con A at 2 µg/ml was added to the culture. For anti-CD3 stimulation, 25% tissue culture supernatant from the 145-2C11 hybridoma was added to the culture. Cells were cultured in presence of syngeneic (C57BL/6) APCs and harvested after 48 h. For the mixed lymphocyte reaction, responder T cells were combined with either syngeneic (C57BL/6) or allogeneic (BALB/c) APCs. Cells were harvested after a total of 96 h.
Flow Cytometry.
For multicolor flow cytometry (FCM), thymocytes or LN cells were first incubated with antibody to the Fc receptor (mAb 2.4G2) to prevent FcR binding. For two- and threecolor FCM, cells were incubated with FITC-conjugated, PEconjugated, and biotinylated antibodies, followed by the addition of red 670 streptavidin (GIBCO BRL, Gaithersburg, MD). The FCM was performed on a Becton Dickinson Immunocytometry Systems FACScan® using standard Cell Quest software. Data were collected on 10–20 x 104 viable cells as determined by forward and side light scatter. The majority of monoclonal antibodies used for FCM analysis were purchased from PharMingen (San Diego, CA) and included biotinylated anti-CD4 (RM4.5) and PE–anti-CD8 (6.7), FITC and PE–anti-CD3 (145-2C11), FITC–anti-TCRVβ14 (14-2), and FITC–anti-V11 (RR8-1). Anti-TCRVβ8 (F23.1) and anti–H-Y clonotypic receptor (T3.70) were purified from cell culture supernatants and labeled with FITC.
Superantigen-induced Thymocyte Deletion.
4–8-wk-old mice were injected intraperitoneally with PBS alone, 10 µg staphylococcus enterotoxin B (SEB; Sigma Chemical Co., St. Louis, MO) in PBS, or 20 µg SEB in PBS, every other day for 1 wk (three doses). 2 d after the final injection, mice were killed and thymocytes were harvested. Thymocytes were stained with anti-Vβ8–FITC or anti-Vβ14–FITC, followed by anti-CD3–PE. Deletion was assessed by software gating on CD3high (SP cells) and determining the percentage of cells expressing individual TCRVβ chains. Percent deletion was determined by dividing the percentage of CD3high, TCRVβ8+ thymocytes remaining after SEB injection by the percentage of CD3high, TCRVβ8+ cells present in mice injected with PBS alone.
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Results
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Reconstitution of –/– mice with a transgene encoding a variant chain lacking all three chain ITAMs (-0 ITAM) restores TCR surface expression and promotes the generation of large numbers of CD4+CD8– and CD4–CD8+ SP thymocytes and peripheral T cells (15). Significantly, the T cells generated in -0 ITAM Tg mice are functionally competent as assessed by their ability to respond to TCR-dependent stimuli (Fig. 1; 23). These results demonstrate that TCR- chain signals are not specifically required for either the generation or activation of mature T cells.
To examine the importance of the multiple TCR- ITAMs in the selection of the T cell repertoire, we fixed the specificity of the TCRs expressed on developing thymocytes by introducing a transgene that encodes productively rearranged TCR- and -β chains specific for the male H-Y antigen in the context of H-2Db (H-Y TCR; 21). This approach enabled us to examine both positive selection (H-2Db female mice) and negative selection (H-2Db male mice) of thymocytes bearing a single /β-TCR.
Examination of –/– mice that express the H-Y transgene revealed that the developmental impairment observed previously in –/– mice was also observed in both male and female H-Y+/–/– mice (Fig. 2). These results were expected because of the extremely low level of TCR surface expression in the absence of chain (11–14) and are consistent with a requirement for the TCR for normal thymocyte development and selection. To address the importance of the chain ITAMs in thymocyte selection, we next examined H-Y+/–/– mice reconstituted with transgenes encoding either full-length chains (H-Y+/-3 ITAM Tg mice), chains containing a single ITAM (H-Y+/-1 ITAM Tg mice), or chains lacking all three ITAMs (H-Y+/- 0 ITAM Tg mice) (15). Each of the transgenes was capable of restoring TCR surface expression in mice lacking endogenous chain (Fig. 3; 15). For these experiments transgenic founder lines were chosen that gave comparable levels of TCR surface expression that were equal to or greater than that found on thymocytes from +/+ mice (Fig. 3; 15).
In female H-2Db, H-Y+/+/+ mice, interaction of the H-Y TCR on immature thymocytes with an unidentified ligand results in positive selection and generation of CD8 SP thymocytes that express high surface levels of the H-Y TCR (detected by reactivity with the clonotype-specific antibody, T3.70; reference 24; Fig. 3 A, Table 1 A). Importantly, a phenotype similar to that of H-Y+/+/+ mice was observed in female H-Y+/–/– mice reconstituted with the full-length transgene (H-Y+/-3 ITAM Tg; Fig. 3 A, Table 1 A). These mice also had large thymi that contained a high percentage of T3.70+, CD8 SP cells indicating that positive selection was effectively restored in –/– mice by expression of a transgene-encoded full-length (3 ITAM) chain (Fig. 3). However, in female H-Y+/–/– mice reconstituted with a transgene encoding the signaling-deficient chain (H-Y+/-0 ITAM Tg), positive selection of T3.70+ thymocytes was markedly impaired as evidenced by the extremely low percentage of T3.70+, CD8 SP thymocytes (Fig. 3 A, Table 1 A). Interestingly, H-Y+/–/– females reconstituted with transgenic chains that contain a single ITAM (either the first [membrane proximal] or third [membrane distal]) exhibited an intermediate phenotype (Fig. 3 A). Although there were subtle differences in the two H-Y+/-1 ITAM Tg lines, the percentage of T3.70+, CD8 SP thymocytes in both lines consistently fell between those observed in mice reconstituted with either the -3 ITAM or -0 ITAM transgenes. The direct relationship between the number of chain ITAMs and the generation of clonotypic (T3.70+) CD8 SP thymocytes was even more evident when absolute numbers of T3.70+, CD8+ thymocytes in the various transgenic lines were compared (Table 1 A). Examination of LNs from H-Y+/-0 ITAM Tg females also revealed a lower percentage of T3.70+, CD8+ T cells relative to H-Y+/-3 ITAM Tg females (Fig. 4). A similar relationship between the number of TCR- ITAMs and the efficiency of positive selection was also observed with a class II–restricted TCR-β Tg (AND; reference 22; Fig. 5). Together, these results demonstrate that TCR ligand interactions that can generate signals that promote positive selection in the presence of full-length chains are unable to generate these signals in the absence of chain ITAMs.
Despite the near absence of T3.70+, CD8+ LN T cells, large numbers of T3.70–, CD8 SP thymocytes and T cells were detected in H-Y+/-0 ITAM Tg females (Figs. 3 A and 4). Since these T3.70– CD8 SP cells expressed /β-TCRs composed of transgenic TCR-β chains paired with non- transgenic, endogenously derived TCR- chains, their TCR specificity was not restricted to the H-Y antigen. Therefore, the reduction in T3.70+, CD8 SP thymocytes and T cells in H-Y+/-0 ITAM Tg mice specifically reflected the failure of the H-Y TCR to promote positive selection of T3.70+ DP thymocytes.
To assess the role of the ITAMs in negative selection, we next examined H-Y+/ Tg male mice. Both H-Y+/+/+ males and H-Y+/-3 ITAM males had small thymi with few (<107) thymocytes which were predominantly CD4– CD8– (Fig. 3 B, Table 1 B). The marked reduction in DP thymocytes in these mice is consistent with previous data indicating that negative selection occurs before the DP stage in H-Y Tg males (25). In contrast to mice expressing full-length chain, H-Y+/-0 ITAM Tg males exhibited a very different phenotype. Thymi from these mice were three- to sixfold larger than H-Y+/-3 ITAM Tg males (Table 1 B) and contained predominantly T3.70+, DP thymocytes (Fig. 3 B). Again, the phenotype exhibited by both lines of H-Y+/-1 ITAM Tg mice was intermediate between H-Y+/-3 ITAM Tg males and H-Y+/-0 ITAM Tg males. For example, thymi from H-Y+/-1 ITAM Tg males contained lower percentages of DP thymocytes than did H-Y+/-0 ITAM Tg males, but contained higher percentages of CD4–CD8low cells which are thought to be the immediate precursors of DP thymocytes (26–28; Fig. 3 B, Table 1 B). Thus, the early negative selection of H-Y clonotypic (T3.70+) thymocytes was attenuated in H-Y+/-0 ITAM Tg and H-Y+/-1 ITAM Tg males.
We next examined LNs to determine the fate of the DP thymocytes observed in male H-Y+/-0 ITAM Tg and H-Y+/-1 ITAM Tg mice. Whereas LN from both H-Y+/ +/+ males and H-Y+/-3 ITAM Tg males contained almost no "conventional" CD4high or CD8high T cells, large numbers of both CD4high and CD8high T cells were present in H-Y+/-0 ITAM Tg (Fig. 4) and H-Y+/-1 ITAM Tg males (data not shown). These CD4high and CD8high T cells expressed high levels of TCR which were uniformly T3.70low/– indicating that they were composed primarily of nontransgenic TCR- chains. Therefore, although negative selection of T3.70+ DP thymocytes was not abrogated in H-Y+/-0 ITAM Tg and H-Y+/-1 ITAM Tg males, some DP thymocytes were able to escape negative selection. Furthermore, these thymocytes were also capable of being positively selected on the basis of non–H-Y restricted TCRs generated from rearrangement and expression of endogenous TCR- genes.
To study the role of -mediated signals in deletion of more mature (SP) thymocytes, we examined superantigenmediated thymocyte deletion in the various transgenic lines. The superantigen SEB deletes SP thymocytes bearing TCRs that include Vβ8 (29). Adult mice were injected with SEB or PBS every other day for 1 wk and the percentage of TCRVβ8+ SP thymocytes remaining was determined by FCM analysis. At similar doses of antigen, the efficiency of SEB-induced thymocyte deletion in -0 ITAM mice was significantly reduced relative to -3 ITAM Tg mice (Fig. 6). Nevertheless, thymocyte deletion by SEB was detectable in -0 ITAM Tg mice and increased with increasing SEB dosage (Fig. 6). The deletion of Vβ8+ thymocytes was specific as the percent TCRVβ14+ SP thymocytes (which are not deleted by SEB) was similar in -0 ITAM Tg and -3 ITAM Tg mice (data not shown). Therefore, in the absence of TCR- signals, deletion of SP thymocytes by SEB was significantly impaired.
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Figure 6 Superantigen-mediated deletion of TCRVβ8+ thymocytes. Mice were injected with PBS alone, 10 µg SEB in PBS, or 20 µg SEB. Thymocytes were stained with anti-Vβ8–FITC or anti-Vβ14–FITC, followed by anti-CD3–PE. Deletion was assessed by software gating on CD3high (SP cells) and determining the percentage of cells expressing individual TCRVβ chains. Deletion was specific for cells expressing Vβ8 as cells expressing Vβ14 were not deleted (29). Results are given as mean ± SEM and are from at least six different SEB injected mice per group compiled from two to three separate experiments.
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Discussion
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In these studies, we investigated the role of the TCR- chain ITAMs in thymocyte selection by fixing the specificity of the TCR and then varying the TCR signaling potential by altering the number of TCR- chain ITAMs within the TCR complex. The results reveal a direct relationship between the number of chain ITAMs in the TCR complex and the efficiency of both positive and negative thymocyte selection. In addition, they suggest that the multiple chain ITAMs function primarily to amplify signals generated by the TCR during thymocyte selection. In this respect, our findings are consistent with in vitro results obtained from mature T cell clones which failed to identify a specific function for individual ITAMs, but found that triplication of a single motif resulted in a quantitative enhancement of signaling (9, 10). However, our current results do not preclude a specific role for individual TCR- ITAMs in more specialized T cell functions that have yet to be examined.
Our results with two /β-TCR Tg systems (H-Y and AND) suggest that positive selection, which is thought to be mediated by low avidity TCR–ligand interactions (2, 16–19) is particularly dependent upon signal amplification by the TCR- chain. In H-Y+/-0 ITAM Tg female mice, most thymocytes that expressed only the H-Y–specific TCR failed to be positively selected, whereas a large number of thymocytes that expressed non–H-Y–specific TCRs were capable of being positively selected. We predict that the mature T cells generated in both non-/β-TCR Tg/-0 ITAM Tg (15) and H-Y+/-0 ITAM Tg mice are selected on the basis of TCRs with relatively high affinity for self ligands, and that these TCRs might otherwise promote negative selection in +/+ or -3 ITAM Tg mice where the TCR contains the full complement of ITAMs. Signal amplification by the chain ITAMs may therefore not be absolutely required for positive selection if the avidity of the positively selecting TCR–ligand interaction is sufficiently high. In these instances, signal transduction by the remaining ITAMs supplied by the CD3 chains (which are targeted to the cell surface in our model system) is apparently adequate for selection in the absence of -mediated signals.
Interestingly, incremenatal reduction in the number of TCR- ITAMs resulted in graded effects on positive selection. Graded or quantitative effects on positive selection have also been observed with increasing concentrations of positively selecting ligand (16–19). These and other (20) data suggest that a broad range of signaling responses below a certain threshold may be capable of mediating positive selection, albeit with different efficiency.
Our results demonstrate that TCR signal amplification also plays an important role in negative selection; however, in H-Y Tg mice, the impact of removing the ITAMs from the TCR complex varied depending on the developmental stage. Negative selection of early "transtitional" (CD8lowCD4–) thymocytes was abrogated in the absence of -mediated signals, presumably because low CD8 surface density lowers the avidity of the ligand interaction (21, 25). These cells could give rise to T3.70+ (CD4highCD8high) DP thymocytes, but DP thymocytes were more susceptible to negative selection as demonstrated by their low numbers in H-Y+/-0 ITAM Tg males. Nevertheless, negative selection was also impaired at the DP stage in H-Y+/-0 ITAM Tg mice to the extent that some DP thymocytes survived, rearranged, and expressed endogenous TCR-, and were positively selected on the basis of non–H-Y clonotypic TCRs. Superantigen-induced deletion of SP thymocytes was also affected in -0 ITAM Tg mice, demonstrating that negative selection which occurs at later stages of thymocyte developement is also impaired in the absence of -mediated signals.
That reduction in the number of TCR ITAMs quantitatively affects both positive and negative selection is consistent with the hypothesis that the outcome (positive or negative) of thymocyte selection is dictated by quantitative rather than qualitative differences in the TCR signaling response (2). Although it might have been predicted, we did not observe positive selection of T3.70+ thymocytes in H-Y+ 0-ITAM Tg males. However, the signaling capacity of the H-Y TCR in H-Y+ 0-ITAM Tg males may not have been sufficiently reduced to observe such an outcome, as negative selection, though impaired, was not abrogated in these mice. Our findings suggest that signals generated from negative selecting interactions are more difficult to attenuate than those generated during positive selection, not only because higher avidity TCR–ligand interactions are thought to promote negative selection, but also because of the effect of TCR signal amplification. Therefore, these results provide an alternative interpretation to studies suggesting that positive and negative selection are biochemically distinguishable based primarily on the finding that approaches that disrupt positive selection fail to perturb negative selection (30, 31).
Finally, it has remained unclear how relatively small differences in the affinity or avidity of TCR self–ligand interactions can differentially lead either to positive or negative selection (32). Our data suggest that the ability of the TCR to elicit such developmentally distinct cell fate decisions stems from its unique structure. Multiple ITAMs may serve to greatly magnify TCR signals and thereby translate relatively small differences in avidity into large signaling differences. Thus, the unique configuration of the TCR, with its capacity for signal amplification, plays a pivitol role in determining the ultimate fate of developing thymocytes and in shaping the mature T cell repertoire.
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Acknowledgments
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We thank B.J Fowlkes, A.S. Rosenberg, A. Singer, and M. Vacchio for reading the manuscript and for helpful discussions.
Submitted: 3 January 1997
1Abbreviations used in this paper: DP, double positive; FCM, flow cytometry; ITAM, immunoreceptor tyrosine-based activation motif; SEB, Staphylococcus enterotoxin B; SP, single positive.
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References
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Abstract
Materials and Methods
