Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents

This Article Full Text Full Text (PDF, 2662K) PPT slides of all figures Supplemental Material Index Alert me when this article is cited Citation Map Services Email this article Similar articles in this journal Similar articles in PubMed Alert me to new content in the JEM Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Weller, S. Articles by Reynaud, C.-A. Search for Related Content PubMed PubMed Citation Articles by Weller, S. Articles by Reynaud, C.-A. Pubmed/NCBI databases Substance via MeSH Related Collections Related Article Social Bookmarking                            What's this?

¹ Institut National de la Santé et de la Recherche Médicale (INSERM) U783, Développement du Système Immunitaire, ² INSERM U550, Génétique des maladies infectieuses, ³ INSERM IFR 94, Service commun de Tri cellulaire, Université Paris Descartes, Faculté de Médecine, Site Necker Enfants-Malades, Paris 75015, France
⁴ Centre d'étude des déficits immunitaires, Hôpital Necker Enfants-Malades, Paris 75015, France
⁵ Service de Chirurgie Pédiatrique, Hôpital Bicêtre, Le Kremlin Bicêtre 94270, France

CORRESPONDENCE Jean-Claude Weill: weill{at}necker.fr OR Claude-Agnès Reynaud: reynaud{at}necker.fr

T cell–dependent immune responses develop soon after birth, whereas it takes 2 yr for humans to develop T cell–independent responses. We used this dissociation to analyze the repertoire diversification of IgM⁺IgD⁺CD27⁺ B cells (also known as "IgM memory" B cells), comparing these cells with switched B cells in children <2 yr of age, with the aim of determining whether these two subsets are developmentally related. We show that the repertoire of IgM⁺IgD⁺CD27⁺ B cells in the spleen and blood displays no sign of antigen-driven activation and expansion on H-CDR3 spectratyping, despite the many antigenic challenges provided by childhood vaccinations. This repertoire differed markedly from those of switched B cells and splenic germinal center B cells, even at the early stage of differentiation associated with µ heavy chain expression. These data provide evidence for the developmental diversification of IgM⁺IgD⁺CD27⁺ B cells, at least in very young children, outside of T cell–dependent and –independent immune responses.

© 2008 Weller et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jgp.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

CiteULike    Complore    Connotea    Del.icio.us    Digg    Facebook    Reddit    Technorati    Twitter    What's this?

Related Article

Sheepish B cells: evidence for antigen-independent antibody diversification in humans and mice

David Tarlinton
J. Exp. Med. 2008 205: 1251-1254. [Abstract] [Full Text] [PDF]

This article has been cited by other articles:

• Kuraoka, M., Liao, D., Yang, K., Allgood, S. D., Levesque, M. C., Kelsoe, G., Ueda, Y. (2009). Activation-Induced Cytidine Deaminase Expression and Activity in the Absence of Germinal Centers: Insights into Hyper-IgM Syndrome. J. Immunol. 183: 3237-3248 [Abstract] [Full Text]  
• Bende, R. J., van Maldegem, F., van Noesel, C. J.M. (2009). Chronic inflammatory disease, lymphoid tissue neogenesis and extranodal marginal zone B-cell lymphomas. haematol 94: 1109-1123 [Abstract] [Full Text]  
• Faili, A., Stary, A., Delbos, F., Weller, S., Aoufouchi, S., Sarasin, A., Weill, J.-C., Reynaud, C.-A. (2009). A Backup Role of DNA Polymerase {kappa} in Ig Gene Hypermutation Only Takes Place in the Complete Absence of DNA Polymerase {eta}. J. Immunol. 182: 6353-6359 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search

TABLE OF CONTENTS