The Journal of Experimental Medicine
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The Journal of Experimental Medicine, Vol 42, 747-752, Copyright, 1925, by The Rockefeller Institute for Medical Research New York

ARTICLE

THE IMMUNIZING ANTIGEN OF THE PNEUMOCOCCUS : III. THE PURIFICATION OF THE WATER-SOLUBLE ANTIGEN.



William A. Perlzweig Ph.D.1 and Chester S. Keefer M.D.1

1 From the Chemical Division of the Medical Clinic of the Johns Hopkins University and Hospital, Baltimore.

Actively immunizing fractions of protein nature have been isolated from broth culture filtrates of Pneumococcus Type I by ultrafiltration, precipitation at a definite hydrion concentration, and the separation of a soluble picrate fraction. The method appears to be suitable for the initial purification of this antigen.

Submitted on August 12, 1925


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