The Journal of Experimental Medicine
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Published online
doi:10.1084/jem.20082232
The Journal of Experimental Medicine, Vol. 206, No. 3, 607-622
The Rockefeller University Press, 0022-1007 $30.00
© Li et al.
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ARTICLE

 Thymus-homing peripheral dendritic cells constitute two of the three major subsets of dendritic cells in the steady-state thymus

JiChu Li1, JooHung Park2, Deborah Foss3, and Irving Goldschneider3

1 Department of Viral Pathogenesis, Beth Israel Deaconess Medical Center, Boston, MA 02215
2 Department of Biology, Changwon National University, Changwon, Kyongnam, 641-773, Korea
3 Department of Immunology, School of Medicine, University of Connecticut Health Center, Farmington, CT 06030

CORRESPONDENCE Irving Goldschneider: igoldsch{at}neuron.uchc.edu

Many dendritic cells (DCs) in the normal mouse thymus are generated intrathymically from common T cell/DC progenitors. However, our previous work suggested that at least 50% of thymic DCs originate independently of these progenitors. We now formally demonstrate by parabiotic, adoptive transfer, and developmental studies that two of the three major subsets of thymic DCs originate extrathymically and continually migrate to the thymus, where they occupy a finite number of microenvironmental niches. The thymus-homing DCs consisted of immature plasmacytoid DCs (pDCs) and the signal regulatory protein {alpha}–positive (Sirp{alpha}+) CD11b+ CD8{alpha}– subset of conventional DCs (cDCs), both of which could take up and transport circulating antigen to the thymus. The cDCs of intrathymic origin were mostly Sirp{alpha}– CD11b– CD8{alpha}hi cells. Upon arrival in the thymus, the migrant pDCs enlarged and up-regulated CD11c, major histocompatibility complex II (MHC II), and CD8{alpha}, but maintained their plasmacytoid morphology. In contrast, the migrant cDCs proliferated extensively, up-regulated CD11c, MHC II, and CD86, and expressed dendritic processes. The possible functional implications of these findings are discussed.

Abbreviations used: 7-AAD, 7-aminoactinomycin D; cDC, conventional DC; CSA, cyclosporin A; eGFP, enhanced GFP; pDC, plasmacytoid DC; Sirp{alpha}, signal regulatory protein {alpha}; tg, transgenic.

© 2009 Li et al.
This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jem.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).


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