The Journal of Experimental Medicine
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Published online
doi:10.1084/jem.20080601
The Journal of Experimental Medicine, Vol. 206, No. 2, 359-370
The Rockefeller University Press, 0022-1007 $30.00
© Lee et al.
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ARTICLE

 Differential roles of migratory and resident DCs in T cell priming after mucosal or skin HSV-1 infection

Heung Kyu Lee1, Melodie Zamora1, Melissa M. Linehan1, Norifumi Iijima1, David Gonzalez2, Ann Haberman3, and Akiko Iwasaki1

1 Department of Immunobiology, 2 Department of Internal Medicine, and 3 Department of Laboratory Medicine, Yale University School of Medicine, New Haven, CT 06520

CORRESPONDENCE Akiko Iwasaki: akiko.iwasaki{at}yale.edu

Although mucosal surfaces represent the main portal of entry for pathogens, the mechanism of antigen presentation by dendritic cells (DCs) that patrol various mucosal tissues remains unclear. Instead, much effort has focused on the understanding of initiation of immune responses generated against antigens delivered by injection. We examined the contributions of migratory versus lymph node–resident DC populations in antigen presentation to CD4 and CD8 T cells after needle injection, epicutaneous infection, or vaginal mucosal herpes simplex virus (HSV) 1 infection. We show that upon needle injection, HSV-1 became lymph-borne and was rapidly presented by lymph node–resident DCs to CD4 and CD8 T cells. In contrast, after vaginal HSV-1 infection, antigens were largely presented by tissue-derived migrant DCs with delayed kinetics. In addition, migrant DCs made more frequent contact with HSV-specific T cells after vaginal infection compared with epicutaneous infection. Thus, both migrant and resident DCs play an important role in priming CD8 and CD4 T cell responses, and their relative importance depends on the mode of infection in vivo.

Abbreviations used: cDC, conventional DC; DN, double negative; EpCAM, epithelial cell adhesion molecule; f.p., footpad; iNOS, inducible NO synthase; ivag, intravaginal; pDC, plasmacytoid DC; PTX, pertussis toxin; TIP DC, TNF/inducible NO synthase–producing DC.

© 2009 Lee et al.
This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jem.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).


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