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¹ Department of Physiology, ² Department of Dentistry and Oral Surgery, ³ Department of Cell Differentiation, the Sakaguchi Laboratory of Developmental Biology, ⁴ Department of Orthopedic Surgery and ⁵ Department of Ophthalmology, Keio University School of Medicine, Shinjuku-ku, Tokyo 160-8582, Japan
⁶ Laboratory for Cell Function and Dynamics, Advanced Technology Development Group, Brain Science Institute, Institute of Physical and Chemical Research, Wako-city, Saitama 351-0198, Japan
⁷ Life Function and Dynamics, Exploratory Research for Advanced Technology Office, Japan Science and Technology Agency, Wako-city, Saitama 351-0198, Japan

CORRESPONDENCE Yumi Matsuzaki: penguin{at}sc.itc.keio.ac.jp

Mesenchymal stem cells (MSCs) are defined as cells that undergo sustained in vitro growth and can give rise to multiple mesenchymal lineages. Because MSCs have only been isolated from tissue in culture, the equivalent cells have not been identified in vivo and little is known about their physiological roles or even their exact tissue location. In this study, we used phenotypic, morphological, and functional criteria to identify and prospectively isolate a subset of MSCs (PDGFR⁺Sca-1⁺CD45^–TER119^–) from adult mouse bone marrow. Individual MSCs generated colonies at a high frequency and could differentiate into hematopoietic niche cells, osteoblasts, and adipocytes after in vivo transplantation. Naive MSCs resided in the perivascular region in a quiescent state. This study provides the useful method needed to identify MSCs as defined in vivo entities.

Abbreviations used: BLI, bioluminescence imaging; BMMNC, BM mononuclear cell; CFU-F, fibroblast CFU; CFU-S, spleen CFU; HSC, hematopoietic stem cell; IHC, immunohistochemistry; mMSC, murine MSC; MPC, mesenchymal progenitor cell; MSC, mesenchymal stem cell; PDGFR, platelet-derived growth factor receptor ; Sca-1, stem cell antigen 1; vSMC, vascular smooth muscle cell.

© 2009 Morikawa et al.
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This article has been cited by other articles:

• Morikawa, S., Mabuchi, Y., Kubota, Y., Nagai, Y., Niibe, K., Hiratsu, E., Suzuki, S., Miyauchi-Hara, C., Nagoshi, N., Sunabori, T., Shimmura, S., Miyawaki, A., Nakagawa, T., Suda, T., Okano, H., Matsuzaki, Y. (2009). Prospective identification, isolation, and systemic transplantation of multipotent mesenchymal stem cells in murine bone marrow. JCB 187: i4-i4 [Full Text]  

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