The Journal of Experimental Medicine
for flow cytometry > invitrogen
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
A correction to this article has been published: Dhodapkar et al., J. Exp. Med. 204 (10) 2489
A correction to this article has been published: Dhodapkar et al., J. Exp. Med. 204 (10) 2494
Published online
doi:10.1084/jem.20062545
The Journal of Experimental Medicine, Vol. 204, No. 6, 1359-1369
The Rockefeller University Press, 0022-1007 $30.00
© Dhodapkar et al.
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 2598K)
Right arrow PPT slides of all figures
Right arrow Supplemental Material Index
Right arrow Correction (v204,p2489)
Right arrow Correction (v204,p2494)
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dhodapkar, K. M.
Right arrow Articles by Dhodapkar, M. V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dhodapkar, K. M.
Right arrow Articles by Dhodapkar, M. V.
Right arrowPubmed/NCBI databases
*Gene*GEO DataSet
*GEO Profiles*HomoloGene
*UniGene
*Substance via MeSH
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

ARTICLE

 Selective blockade of the inhibitory Fc{gamma} receptor (Fc{gamma}RIIB) in human dendritic cells and monocytes induces a type I interferon response program

Kavita M. Dhodapkar1, Devi Banerjee1, John Connolly6, Anjli Kukreja3, Elyana Matayeva1, Maria Concetta Veri5, Jeffrey V. Ravetch2,4, Ralph M. Steinman1,4, and Madhav V. Dhodapkar3,4

1 Lab of Cellular Physiology and Immunology, 2 Lab of Molecular Genetics and Immunology, 3 Lab of Tumor Immunology and Immunotherapy, and 4 Chris Browne Center for Immunology, The Rockefeller University, New York, NY 10021
5 MacroGenics, Inc., Rockville, MD 20850
6 Baylor Institute of Immunology Research, Dallas, TX 75204

CORRESPONDENCE Kavita M. Dhodapkar: dhodapk{at}rockefeller.edu

The ability of dendritic cells (DCs) to activate immunity is linked to their maturation status. In prior studies, we have shown that selective antibody-mediated blockade of inhibitory Fc{gamma}RIIB receptor on human DCs in the presence of activating immunoglobulin (Ig) ligands leads to DC maturation and enhanced immunity to antibody-coated tumor cells. We show that Fc{gamma} receptor (Fc{gamma}R)–mediated activation of human monocytes and monocyte-derived DCs is associated with a distinct gene expression pattern, including several inflammation-associated chemokines, as well as type 1 interferon (IFN) response genes, including the activation of signal transducer and activator of transcription 1 (STAT1). Fc{gamma}R-mediated STAT1 activation is rapid and requires activating Fc{gamma}Rs. However, this IFN response is observed without a detectable increase in the expression of type I IFNs themselves or the need to add exogenous IFNs. Induction of IFN response genes plays an important role in Fc{gamma}R-mediated effects on DCs, as suppression of STAT1 by RNA interference inhibited Fc{gamma}R-mediated DC maturation. These data suggest that the balance of activating/inhibitory Fc{gamma}Rs may regulate IFN signaling in myeloid cells. Manipulation of Fc{gamma}R balance on DCs and monocytes may provide a novel approach to regulating IFN-mediated pathways in autoimmunity and human cancer.

Abbreviations used: Fc{gamma}R, Fc{gamma} receptor; GEP, gene expression profile; IC, immune complex; IDC, immature DC; IFI, IFN-{alpha} inducible; IFNAR, IFN-{alpha} receptor; IRG, IFN response gene; STAT, signal transducer and activator of transcription.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS