The Journal of Experimental Medicine
VeriKine-HS Human IFN-Beta
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online
doi:10.1084/jem.20070176
The Journal of Experimental Medicine, Vol. 204, No. 5, 1095-1106
The Rockefeller University Press, 0022-1007 $30.00
© Soares et al.
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 3568K)
Right arrow PPT slides of all figures
Right arrow Supplemental Material Index
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Soares, H.
Right arrow Articles by Steinman, R. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Soares, H.
Right arrow Articles by Steinman, R. M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

ARTICLE

 A subset of dendritic cells induces CD4+ T cells to produce IFN-{gamma} by an IL-12–independent but CD70-dependent mechanism in vivo

Helena Soares1, HaeNa Waechter1, Nicholas Glaichenhaus3, Evelyne Mougneau3, Hideo Yagita4, Olga Mizenina1, Diana Dudziak2, Michel C. Nussenzweig2, and Ralph M. Steinman1

1 Laboratory of Cellular Physiology and Immunology and Chris Browne Center, and 2 Laboratory of Molecular Immunology and the Howard Hughes Institute, The Rockefeller University, New York, NY 10021
3 E03-44, Institut National de la Sainté et de la Recherche Médicale, University of Nice-Sophia Antipolis, 06560 Valbonne, France
4 Department of Immunology, Juntendo University School of Medicine, Tokyo 113-8421, Japan

CORRESPONDENCE Ralph M. Steinman: steinma{at}mail.rockefeller.edu

Interferon (IFN)-{gamma}, a cytokine critical for resistance to infection and tumors, is produced by CD4+ helper T lymphocytes after stimulation by cultured dendritic cells (DCs) that secrete a cofactor, interleukin (IL)-12. We have identified a major IL-12–independent pathway whereby DCs induce IFN-{gamma}–secreting T helper (Th)1 CD4+ T cells in vivo. This pathway requires the membrane-associated tumor necrosis family member CD70 and was identified by targeting the LACK antigen from Leishmania major within an antibody to CD205 (DEC-205), an uptake receptor on a subset of DCs. Another major DC subset, targeted with 33D1 anti-DCIR2 antibody, also induced IFN-{gamma} in vivo but required IL-12, not CD70. Isolated CD205+ DCs expressed cell surface CD70 when presenting antigen to T cell receptor transgenic T cells, and this distinction was independent of maturation stimuli. CD70 was also essential for CD205+ DC function in vivo. Detection of the IL-12–independent IFN-{gamma} pathway was obscured with nontargeted LACK, which was presented by both DC subsets. This in situ analysis points to CD70 as a decision maker for Th1 differentiation by CD205+ DCs, even in Th2-prone BALB/c animals and potentially in vaccine design. The results indicate that two DC subsets have innate propensities to differentially affect the Th1/Th2 balance in vivo and by distinct mechanisms.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS