The Journal of Experimental Medicine
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Published online
doi:10.1084/jem.20061301
The Journal of Experimental Medicine, Vol. 204, No. 4, 793-804
The Rockefeller University Press, 0022-1007 $30.00
© Jaillon et al.
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ARTICLE

 The humoral pattern recognition receptor PTX3 is stored in neutrophil granules and localizes in extracellular traps

Sébastien Jaillon1, Giuseppe Peri2, Yves Delneste1, Isabelle Frémaux1, Andrea Doni2, Federica Moalli2, Cecilia Garlanda2, Luigina Romani3, Hugues Gascan4, Silvia Bellocchio3, Silvia Bozza3, Marco A. Cassatella5, Pascale Jeannin1,6, and Alberto Mantovani2,7

1 Institut National de la Santé et de la Recherche Médicale, Equipe Avenir, Unité 564, University Hospital of Angers, University of Angers, Angers 49933, France
2 Istituto Clinico Humanitas, Istituto di Ricovero e Cura a Carattere Scientifico, 20089 Rozzano, Italy
3 Microbiology Section, Department of Experimental Medicine and Biochemical Sciences, University of Perugia, 05122 Perugia, Italy
4 Institut National de la Santé et de la Recherche Médicale, Unité 564, University of Angers, 49933 Angers, France
5 Department of Pathology, University of Verona, 37134 Verona, Italy
6 Immunology and Allergology Laboratory, University Hospital of Angers, 49933 Angers, France
7 Institute of General Pathology, Faculty of Medicine, University of Milan, 20100 Milan, Italy

CORRESPONDENCE Alberto Mantovani: alberto.mantovani{at}humanitas.it OR Pascale Jeannin: pascale.jeannin{at}univ-angers.fr

The long pentraxin (PTX) 3 is produced by macrophages and myeloid dendritic cells in response to Toll-like receptor agonists and represents a nonredundant component of humoral innate immunity against selected pathogens. We report that, unexpectedly, PTX3 is stored in specific granules and undergoes release in response to microbial recognition and inflammatory signals. Released PTX3 can partially localize in neutrophil extracellular traps formed by extruded DNA. Eosinophils and basophils do not contain preformed PTX3. PTX3-deficient neutrophils have defective microbial recognition and phagocytosis, and PTX3 is nonredundant for neutrophil-mediated resistance against Aspergillus fumigatus. Thus, neutrophils serve as a reservoir, ready for rapid release, of the long PTX3, a key component of humoral innate immunity with opsonic activity.

Abbreviations used: CHO, Chinese hamster ovary; CRP, C-reactive protein; MMP-9, matrix metalloproteinase 9; MPO, myeloperoxidase; NET, neutrophil extracellular trap; OmpA, outer membrane protein A; PRR, pattern recognition receptor; PTX, pentraxin; SAP, serum amyloid P component; TLR, Toll-like receptor.

S. Jaillon, G. Peri, P. Jeannin, and A. Mantovani contributed equally to this work.


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