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¹ Universite Bordeaux 2, Laboratoire de Physiologie Cellulaire Respiratoire, F-33076 Bordeaux, France
Institut National de la Santé et de la Recherche Médicale (INSERM), ² U885 and ³ U688, F-33076 Bordeaux, France
⁴ Centre Hospitalier Universitaire de Bordeaux, F-33076 Bordeaux, France

CORRESPONDENCE Patrick Berger: patrick.berger{at}u-bordeaux2.fr

Asthma and chronic obstructive pulmonary disease (COPD) are characterized by different patterns of airway remodeling, which all include an increased mass of bronchial smooth muscle (BSM). A remaining major question concerns the mechanisms underlying such a remodeling of BSM. Because mitochondria play a major role in both cell proliferation and apoptosis, we hypothesized that mitochondrial activation in BSM could play a role in this remodeling. We describe that both the mitochondrial mass and oxygen consumption were higher in the BSM from asthmatic subjects than in that from both COPD and controls. This feature, which is specific to asthma, was related to an enhanced mitochondrial biogenesis through up-regulation of peroxisome proliferator-activated receptor coactivator (PGC)–1, nuclear respiratory factor-1, and mitochondrial transcription factor A. The priming event of such activation was an alteration in BSM calcium homeostasis. BSM cell apoptosis was not different in the three groups of subjects. Asthmatic BSM was, however, characterized by increased cell growth and proliferation. Both characteristics were completely abrogated in mitochondria-deficient asthmatic BSM cells. Conversely, in both COPD and control BSM cells, induction of mitochondrial biogenesis reproduced these characteristics. Thus, BSM in asthmatic patients is characterized by an altered calcium homeostasis that increases mitochondrial biogenesis, which, in turn, enhances cell proliferation, leading to airway remodeling.

Abbreviations used: ANOVA, analysis of variance; BSM, bronchial smooth muscle; CaMK-IV, calcium/calmodulin-dependent protein kinase IV; COPD, chronic obstructive pulmonary disease; mtTFA, mitochondrial transcription factor A; NRF, nuclear respiratory factor; PGC, peroxisome proliferator-activated receptor coactivator.

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