The Journal of Experimental Medicine
Torrey Pines Biolabs
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online
doi:10.1084/jem.20070773
The Journal of Experimental Medicine, Vol. 204, No. 12, 2865-2874
The Rockefeller University Press, 0022-1007 $30.00
© Nagamachi et al.
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 1887K)
Right arrow PPT slides of all figures
Right arrow Supplemental Material Index
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nagamachi, M.
Right arrow Articles by Narumiya, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nagamachi, M.
Right arrow Articles by Narumiya, S.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

ARTICLE

 Facilitation of Th1-mediated immune response by prostaglandin E receptor EP1

Miyako Nagamachi1,2, Daiji Sakata1, Kenji Kabashima1,2, Tomoyuki Furuyashiki1, Takahiko Murata1, Eri Segi-Nishida1, Kitipong Soontrapa1, Toshiyuki Matsuoka1, Yoshiki Miyachi2, and Shuh Narumiya1

1 Department of Pharmacology and 2 Department of Dermatology, Faculty of Medicine, Kyoto University, Kyoto 606-8501, Japan

CORRESPONDENCE Shuh Narumiya: snaru{at}mfour.med.kyoto-u.ac.jp

Prostaglandin E2 (PGE2) exerts its actions via four subtypes of the PGE receptor, EP1–4. We show that mice deficient in EP1 exhibited significantly attenuated Th1 response in contact hypersensitivity induced by dinitrofluorobenzene (DNFB). This phenotype was recapitulated in wild-type mice by administration of an EP1-selective antagonist during the sensitization phase, and by adoptive transfer of T cells from sensitized EP1–/– mice. Conversely, an EP1-selective agonist facilitated Th1 differentiation of naive T cells in vitro. Finally, CD11c+ cells containing the inducible form of PGE synthase increased in number in the draining lymph nodes after DNFB application. These results suggest that PGE2 produced by dendritic cells in the lymph nodes acts on EP1 in naive T cells to promote Th1 differentiation.

Abbreviations used: CHS, contact hypersensitivity; COX, cyclooxygenase; cPGES, cytosolic PGE synthase; DNBS, dinitrobenzene sulfonic acid; DNFB, dinitrofluorobenzene; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; LC, Langerhans cell; mPGES-1, membrane-associated PGE synthase-1; PG, prostaglandin; PGES, PGE synthase; T-bet, T-box-expressed-in-T cells.

M. Nagamachi, D. Sakata, and K. Kabashima contributed equally to this work.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS