The Journal of Experimental Medicine
Torrey Pines Biolabs
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online
doi:10.1084/jem.20071006
The Journal of Experimental Medicine, Vol. 204, No. 11, 2655-2665
The Rockefeller University Press, 0022-1007 $30.00
© Bende et al.
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 3991K)
Right arrow PPT slides of all figures
Right arrow Supplemental Material Index
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bende, R. J.
Right arrow Articles by van Noesel, C. J.M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bende, R. J.
Right arrow Articles by van Noesel, C. J.M.
Related Collections
Right arrowRelated Article
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

ARTICLE

 Germinal centers in human lymph nodes contain reactivated memory B cells

Richard J. Bende, Febe van Maldegem, Martijn Triesscheijn, Thera A.M. Wormhoudt, Richard Guijt, and Carel J.M. van Noesel

Department of Pathology, Academic Medical Center, 1105 AZ Amsterdam, Netherlands

CORRESPONDENCE C.J.M. van Noesel: c.j.vannoesel{at}amc.uva.nl

To reveal migration trails of antigen-responsive B cells in lymphoid tissue, we analyzed immunoglobulin (Ig)M-VH and IgG-VH transcripts of germinal center (GC) samples microdissected from three reactive human lymph nodes. Single B cell clones were found in multiple GCs, one clone even in as many as 19 GCs. In several GCs, IgM and IgG variants of the same clonal origin were identified. The offspring of individual hypermutated IgG memory clones were traced in multiple GCs, indicating repeated engagement of memory B cells in GC reactions. These findings imply that recurring somatic hypermutation progressively drives the Ig repertoire of memory B cells to higher affinities and infer that transforming genetic hits in non-Ig genes during lymphomagenesis do not have to arise during a single GC passage, but can be collected during successive recall responses.

Abbreviations used: Ag, antigen(ic); CDR3, complementarity determining region 3; CSR, class-switch recombination; FM, follicular mantle zone; GC, germinal center; IgV, Ig variable region; SHM, somatic hypermutation; TZ, T cell zone.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?

Related Article

Germinal centers are free for all
Hema Bashyam
J. Exp. Med. 2007 204: 2497. [Full Text] [PDF]



This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS