The Journal of Experimental Medicine
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Published online 5 June 2006 doi:10.1084/jem.20052217
Rockefeller University Press, 0022-1007 $8.00
JEM, Volume 203, Number 6, 1567-1578
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ARTICLE

 Forced usage of positively charged amino acids in immunoglobulin CDR-H3 impairs B cell development and antibody production

Gregory C. Ippolito1, Robert L. Schelonka1, Michael Zemlin1, Ivaylo I. Ivanov1, Ryoki Kobayashi1, Cosima Zemlin1, G. Larry Gartland1, Lars Nitschke2, Jukka Pelkonen3, Kohtaro Fujihashi1, Klaus Rajewsky4, and Harry W. Schroeder, Jr.1

1 Department of Microbiology, Department of Pediatrics, Department of Medicine, Department of Pediatric Dentistry, and Department of Genetics, University of Alabama at Birmingham, SHEL 401, Birmingham, AL 35294
2 Department of Genetics, University of Erlangen, 91058 Erlangen, Germany
3 Department of Clinical Microbiology, University of Kuopio, POB 1627, 70211 Kuopio, Finland
4 The Center for Blood Research, Harvard Medical School, Boston, MA 02115

CORRESPONDENCE Harry W. Schroeder Jr.: hwsj{at}uab.edu

Tyrosine and glycine constitute 40% of complementarity determining region 3 of the immunoglobulin heavy chain (CDR-H3), the center of the classic antigen-binding site. To assess the role of DH RF1-encoded tyrosine and glycine in regulating CDR-H3 content and potentially influencing B cell function, we created mice limited to a single DH encoding asparagine, histidine, and arginines in RF1. Tyrosine and glycine content in CDR-H3 was halved. Bone marrow and spleen mature B cell and peritoneal cavity B-1 cell numbers were also halved, whereas marginal zone B cell numbers increased. Serum immunoglobulin G subclass levels and antibody titers to T-dependent and T-independent antigens all declined. Thus, violation of the conserved preference for tyrosine and glycine in DH RF1 alters CDR-H3 content and impairs B cell development and antibody production.

Abbreviations used: {Delta}D-DFL, depleted DH locus with single DFL16.1 gene segment; {Delta}D-iD, depleted DH locus with a single, mutated DFL16.1 gene segment containing inverted DSP 2.2 sequence; CDR-H3, complementarity determining region 3 of the immunoglobulin heavy chain; DEX, {alpha}(1->3)-dextran; i-RF, inverted DH reading frame; NP19-CGG, [4-hydroxy-3-nitrophenyl] acetyl-chicken {gamma} globulin; RF, reading frame; TT, tetanus toxin.


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