The Journal of Experimental Medicine
for flow cytometry > invitrogen
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online 1 May 2006 doi:10.1084/jem.20060418
Rockefeller University Press, 0022-1007 $8.00
JEM, Volume 203, Number 5, 1197-1207
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 735K)
Right arrow PPT slides of all figures
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wei, D. G.
Right arrow Articles by Bendelac, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wei, D. G.
Right arrow Articles by Bendelac, A.
Right arrowPubmed/NCBI databases
*Gene*GEO Profiles
*HomoloGene*UniGene
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

ARTICLE

 Mechanisms imposing the Vß bias of V{alpha}14 natural killer T cells and consequences for microbial glycolipid recognition

Datsen G. Wei1,3, Shane A. Curran1, Paul B. Savage4, Luc Teyton5, and Albert Bendelac1,2

1 Committee on Immunology and 2 Howard Hughes Medical Institute, University of Chicago, Chicago, IL 60637
3 Department of Molecular Biology, Princeton University, Princeton, NJ 08544
4 Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602
5 Department of Immunology, The Scripps Research Institute, La Jolla, CA 92037

CORRESPONDENCE Albert Bendelac: abendela{at}bsd.uchicago.edu

Mouse and human natural killer T (NKT) cells recognize a restricted set of glycosphingolipids presented by CD1d molecules, including self iGb3 and microbial {alpha}-glycuronosylceramides. The importance of the canonical V{alpha}14-J{alpha}18 TCR {alpha} chain for antigen recognition by NKT cells is well recognized, but the mechanisms underlying the Vß8, Vß7, and Vß2 bias in mouse have not been explored. To study the influences of thymic selection and the constraints of pairing with V{alpha}14-J{alpha}18, we have created a population of mature T cells expressing V{alpha}14-J{alpha}18 TCR {alpha} chain in CD1d-deficient mice and studied its recognition properties in vitro and in vivo. Transgenic cells expressed a diverse Vß repertoire but their recognition of endogenous ligands and synthetic iGb3 was restricted to the same biased Vß repertoire as expressed in natural NKT cells. In contrast, {alpha}-GalCer, a synthetic homologue of microbial {alpha}-glycuronosylceramides, was recognized by a broader set of Vß chains, including the biased NKT set but also Vß6, Vß9, Vß10, and Vß14. These surprising findings demonstrate that, whereas Vß8, Vß7, and Vß2 represent the optimal solution for recognition of endogenous ligand, many Vß chains that are potentially useful for the recognition of foreign lipids fail to be selected in the NKT cell repertoire.

Abbreviations used: DN, double negative; DP, double positive; NKT, natural killer T; Tg, transgenic.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS