The Journal of Experimental Medicine
ELISpot, FluoroSpot and ELISA kits from Mabtech
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online December 11, 2006
doi:10.1084/jem.20052246
The Journal of Experimental Medicine, Vol. 203, No. 13, 2865-2877
The Rockefeller University Press, 0022-1007 $30.00
© 2006 Casazza et al.
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 3747K)
Right arrow PPT slides of all figures
Right arrow Supplemental Material Index
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Casazza, J. P.
Right arrow Articles by Koup, R. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Casazza, J. P.
Right arrow Articles by Koup, R. A.
Right arrowPubmed/NCBI databases
*Compound via MeSH
*Substance via MeSH
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

ARTICLE

 Acquisition of direct antiviral effector functions by CMV-specific CD4+ T lymphocytes with cellular maturation

Joseph P. Casazza1, Michael R. Betts1,4, David A. Price2, Melissa L. Precopio1, Laura E. Ruff2, Jason M. Brenchley2, Brenna J. Hill2, Mario Roederer3, Daniel C. Douek2, and Richard A. Koup1

1 Immunology Laboratory, 2 Human Immunology Section, and 3 Immunotechnology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, MD 20892
4 Department of Microbiology, University of Pennsylvania, Philadelphia, PA 19104

CORRESPONDENCE Richard A. Koup: rkoup{at}mail.nih.gov

The role of CD4+ T cells in the control of persistent viral infections beyond the provision of cognate help remains unclear. We used polychromatic flow cytometry to evaluate the production of the cytokines interferon (IFN)-{gamma}, tumor necrosis factor (TNF)-{alpha}, and interleukin (IL)-2, the chemokine macrophage inflammatory protein (MIP)-1ß, and surface mobilization of the degranulation marker CD107a by CD4+ T cells in response to stimulation with cytomegalovirus (CMV)-specific major histocompatibility complex class II peptide epitopes. Surface expression of CD45RO, CD27, and CD57 on responding cells was used to classify CD4+ T cell maturation. The functional profile of virus-specific CD4+ T cells in chronic CMV infection was unique compared with that observed in other viral infections. Salient features of this profile were: (a) the simultaneous production of MIP-1ß, TNF-{alpha}, and IFN-{gamma} in the absence of IL-2; and (b) direct cytolytic activity associated with surface mobilization of CD107a and intracellular expression of perforin and granzymes. This polyfunctional profile was associated with a terminally differentiated phenotype that was not characterized by a distinct clonotypic composition. Thus, mature CMV-specific CD4+ T cells exhibit distinct functional properties reminiscent of antiviral CD8+ T lymphocytes.

Abbreviations used: B-LCL, B lymphoblastoid cell; CMTMR, chloromethyl-benzoyl-aminotetramethyl-rhodamine; MIP, macrophage inflammatory protein.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS