The Journal of Experimental Medicine
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Published online 27 December 2005 doi:10.1084/jem.20050903
Rockefeller University Press, 0022-1007 $8.00
JEM, Volume 203, Number 1, 119-129
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ARTICLE

 LAT-mediated signaling in CD4+CD25+ regulatory T cell development

Surapong Koonpaew, Shudan Shen, Lawrence Flowers, and Weiguo Zhang

Department of Immunology, Duke University Medical Center, Durham, NC 27710

CORRESPONDENCE: Weiguo Zhang: zhang033{at}mc.duke.edu

Engagement of the T cell receptor for antigen (TCR) induces formation of signaling complexes mediated through the transmembrane adaptor protein, the linker for activation of T cells (LAT). LAT plays an important role in T cell development, activation, and homeostasis. A knock-in mutation at Tyr136, which is the phospholipase C (PLC)-{gamma}1–binding site in LAT, leads to a severe autoimmune disease in mice. In this study, we show that CD4+CD25+ T reg cells that expressed Foxp3 transcription factor were nearly absent in both thymus and peripheral lymphoid organs of LATY136F mice. This defect was not a result of the autoimmune environment as LATY136F T reg cells also failed to develop in healthy LAT–/– mice that received mixed wild-type and LATY136F bone marrow cells. Moreover, adoptive transfer of normal CD4+CD25+ T reg cells protected neonatal LATY136F mice from developing this disease. These T reg cells effectively controlled expansion of CD4+ T cells in LATY136F mice likely via granzymes and/or TGF-ß–mediated suppression. Furthermore, ectopic expression of Foxp3 conferred a suppressive function in LATY136F T cells. Our data indicate that the LAT–PLC-{gamma}1 interaction plays a critical role in Foxp3 expression and the development of CD4+CD25+ T reg cells

Abbreviations used: GFP, green fluorescence protein; GITR, glucocorticoid-induced TNF receptor; LAT, linker for activation of T cells; PLC, phospholipase C.


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