The Journal of Experimental Medicine
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Published online 31 October 2005 doi:10.1084/jem.20051038
Rockefeller University Press, 0022-1007 $8.00
JEM, Volume 202, Number 9, 1225-1234
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ARTICLE

 Characterization of marginal zone B cell precursors

Bhaskar Srivastava1, William J. Quinn, III1, Kristin Hazard2, Jan Erikson2, and David Allman1

1 Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine
2 The Wistar Institute, Philadelphia, PA 19104

CORRESPONDENCE David Allman: dallman{at}mail.med.upenn.edu

Selection of recently formed B cells into the follicular or marginal zone (MZ) compartments is proposed to occur by way of proliferative intermediates expressing high levels of CD21/35 and CD23. However, we show that CD21/35high CD23+ splenocytes are not enriched for proliferative cells, and do not contribute substantially to the generation of follicular B cells. Instead, ontogenic relationships, steady-state labeling kinetics, and adoptive transfer experiments suggest that CD21/35high CD23+ splenocytes serve primarily as precursors for MZ B cells, although their developmental potential seems to be broader and is influenced by environmental cues that are associated with lymphopenia. Furthermore, CD21/35high CD23+ splenocytes share several key functional characteristics with MZ B cells, including their capacity to trap T-independent antigen and a heightened proliferative response to LPS. These observations challenge previous models of peripheral B cell maturation, and suggest that MZ B cells develop by way of CD21/35high CD23+ intermediates.

Abbreviations used: BCR, B cell receptor; BI, biotin; CFSE, carboxyl fluorescein succinimidyl ester; Fr., fraction; MZ, marginal zone; SA, streptavidin; VCAM, vascular cell adhesion molecule.


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