Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents

This Article Full Text Full Text (PDF, 2745K) PPT slides of all figures Alert me when this article is cited Citation Map Services Email this article Similar articles in this journal Similar articles in PubMed Alert me to new content in the JEM Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Johansson-Lindbom, B. Articles by Agace, W. W. Search for Related Content PubMed PubMed Citation Articles by Johansson-Lindbom, B. Articles by Agace, W. W. Pubmed/NCBI databases Gene GEO Profiles HomoloGene UniGene Substance via MeSH Related Collections Related Articles Social Bookmarking                      What's this?

¹ Immunology Section, Lund University, BMC I-13, 221 84 Lund, Sweden
² Institute of Immunology, Hannover Medical School, 30625 Hannover, Germany
³ Departamento de Immunologia y Oncologia, Centro Nacional de Biotecnologia/Consejo Superior de Investigaciones Cientificas, Universidad Autonoma de Madrid, Cantoblanco, 28040 Madrid, Spain

CORRESPONDENCE William W. Agace: William.Agace{at}immuno.lu.se

Gut-associated lymphoid tissue (GALT) dendritic cells (DCs) display a unique ability to generate CCR9⁺₄ß₇⁺ gut-tropic CD8⁺ effector T cells. We demonstrate efficient induction of CCR9 and ₄ß₇ on CD8⁺ T cells in mesenteric lymph nodes (MLNs) after oral but not intraperitoneal (i.p.) antigen administration indicating differential targeting of DCs via the oral route. In vitro, lamina propria (LP)–derived DCs were more potent than MLN or Peyer's patch DCs in their ability to generate CCR9⁺₄ß₇⁺ CD8⁺ T cells. The integrin chain CD103 (_E) was expressed on almost all LP DCs, a subset of MLN DCs, but on few splenic DCs. CD103⁺ MLN DCs were reduced in number in CCR7^–/– mice and, although CD8⁺ T cells proliferated in the MLNs of CCR7^–/– mice after i.p. but not oral antigen administration, they failed to express CCR9 and had reduced levels of ₄ß₇. Strikingly, although CD103⁺ and CD103^– MLN DCs were equally potent at inducing CD8⁺ T cell proliferation and IFN- production, only CD103⁺ DCs were capable of generating gut-tropic CD8⁺ effector T cells in vitro. Collectively, these results demonstrate a unique function for LP-derived CD103⁺ MLN DCs in the generation of gut-tropic effector T cells.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

Related Articles

Essential role for CD103 in the T cell–mediated regulation of experimental colitis

Oliver Annacker, Janine L. Coombes, Vivianne Malmstrom, Holm H. Uhlig, Tim Bourne, Bengt Johansson-Lindbom, William W. Agace, Christina M. Parker, and Fiona Powrie
J. Exp. Med. 2005 202: 1051-1061. [Abstract] [Full Text] [PDF]

This article has been cited by other articles:

• Stavnezer, J., Kang, J. (2009). The Surprising Discovery That TGF{beta} Specifically Induces the IgA Class Switch. J. Immunol. 182: 5-7 [Full Text]  
• del Rio, M.-L., Rodriguez-Barbosa, J.-I., Bolter, J., Ballmaier, M., Dittrich-Breiholz, O., Kracht, M., Jung, S., Forster, R. (2008). CX3CR1+c-kit+ Bone Marrow Cells Give Rise to CD103+ and CD103- Dendritic Cells with Distinct Functional Properties. J. Immunol. 181: 6178-6188 [Abstract] [Full Text]  
• Behrends, J., Karsten, C. M., Wilke, S., Robke, A., Kruse, A. (2008). Identification of ITGA4/ITGB7 and ITGAE/ITGB7 Expressing Subsets of Decidual Dendritic-Like Cells Within Distinct Microdomains of the Pregnant Mouse Uterus. Biol. Reprod. 79: 624-632 [Abstract] [Full Text]  
• Edele, F., Molenaar, R., Gutle, D., Dudda, J. C., Jakob, T., Homey, B., Mebius, R., Hornef, M., Martin, S. F. (2008). Cutting Edge: Instructive Role of Peripheral Tissue Cells in the Imprinting of T Cell Homing Receptor Patterns. J. Immunol. 181: 3745-3749 [Abstract] [Full Text]  
• Kaufman, D. R., Liu, J., Carville, A., Mansfield, K. G., Havenga, M. J. E., Goudsmit, J., Barouch, D. H. (2008). Trafficking of Antigen-Specific CD8+ T Lymphocytes to Mucosal Surfaces following Intramuscular Vaccination. J. Immunol. 181: 4188-4198 [Abstract] [Full Text]  
• Jaensson, E., Uronen-Hansson, H., Pabst, O., Eksteen, B., Tian, J., Coombes, J. L., Berg, P.-L., Davidsson, T., Powrie, F., Johansson-Lindbom, B., Agace, W. W. (2008). Small intestinal CD103+ dendritic cells display unique functional properties that are conserved between mice and humans. JEM 205: 2139-2149 [Abstract] [Full Text]  
• Ahrendt, M., Hammerschmidt, S. I., Pabst, O., Pabst, R., Bode, U. (2008). Stromal Cells Confer Lymph Node-Specific Properties by Shaping a Unique Microenvironment Influencing Local Immune Responses. J. Immunol. 181: 1898-1907 [Abstract] [Full Text]  
• Schlickum, S., Sennefelder, H., Friedrich, M., Harms, G., Lohse, M. J., Kilshaw, P., Schon, M. P. (2008). Integrin {alpha}E(CD103){beta}7 influences cellular shape and motility in a ligand-dependent fashion. Blood 112: 619-625 [Abstract] [Full Text]  
• Elgueta, R., Sepulveda, F. E., Vilches, F., Vargas, L., Mora, J. R., Bono, M. R., Rosemblatt, M. (2008). Imprinting of CCR9 on CD4 T Cells Requires IL-4 Signaling on Mesenteric Lymph Node Dendritic Cells. J. Immunol. 180: 6501-6507 [Abstract] [Full Text]  
• Miles, A., Liaskou, E., Eksteen, B., Lalor, P. F., Adams, D. H. (2008). CCL25 and CCL28 promote {alpha}4{beta}7-integrin-dependent adhesion of lymphocytes to MAdCAM-1 under shear flow. Am. J. Physiol. Gastrointest. Liver Physiol. 294: G1257-G1267 [Abstract] [Full Text]  
• Blazquez, A. B., Berin, M. C. (2008). Gastrointestinal Dendritic Cells Promote Th2 Skewing via OX40L. J. Immunol. 180: 4441-4450 [Abstract] [Full Text]  
• Beilhack, A., Schulz, S., Baker, J., Beilhack, G. F., Nishimura, R., Baker, E. M., Landan, G., Herman, E. I., Butcher, E. C., Contag, C. H., Negrin, R. S. (2008). Prevention of acute graft-versus-host disease by blocking T-cell entry to secondary lymphoid organs. Blood 111: 2919-2928 [Abstract] [Full Text]  
• Amersi, F. F., Terando, A. M., Goto, Y., Scolyer, R. A., Thompson, J. F., Tran, A. N., Faries, M. B., Morton, D. L., Hoon, D. S.B. (2008). Activation of CCR9/CCL25 in Cutaneous Melanoma Mediates Preferential Metastasis to the Small Intestine. Clin. Cancer Res. 14: 638-645 [Abstract] [Full Text]  
• Knight, A. K., Blazquez, A. B., Zhang, S., Mayer, L., Sampson, H. A., Berin, M. C. (2007). CD4 T cells activated in the mesenteric lymph node mediate gastrointestinal food allergy in mice. Am. J. Physiol. Gastrointest. Liver Physiol. 293: G1234-G1243 [Abstract] [Full Text]  
• Pals, S. T., de Gorter, D. J. J., Spaargaren, M. (2007). Lymphoma dissemination: the other face of lymphocyte homing. Blood 110: 3102-3111 [Abstract] [Full Text]  
• Pabst, O., Bernhardt, G., Forster, R. (2007). The impact of cell-bound antigen transport on mucosal tolerance induction. J. Leukoc. Biol. 82: 795-800 [Abstract] [Full Text]  
• Iliev, I. D., Matteoli, G., Rescigno, M. (2007). The yin and yang of intestinal epithelial cells in controlling dendritic cell function. JEM 204: 2253-2257 [Abstract] [Full Text]  
• Saurer, L., McCullough, K. C., Summerfield, A. (2007). In Vitro Induction of Mucosa-Type Dendritic Cells by All-Trans Retinoic Acid. J. Immunol. 179: 3504-3514 [Abstract] [Full Text]  
• Coombes, J. L., Siddiqui, K. R.R., Arancibia-Carcamo, C. V., Hall, J., Sun, C.-M., Belkaid, Y., Powrie, F. (2007). A functionally specialized population of mucosal CD103+ DCs induces Foxp3+ regulatory T cells via a TGF-{beta} and retinoic acid dependent mechanism. JEM 204: 1757-1764 [Abstract] [Full Text]  
• Sun, C.-M., Hall, J. A., Blank, R. B., Bouladoux, N., Oukka, M., Mora, J. R., Belkaid, Y. (2007). Small intestine lamina propria dendritic cells promote de novo generation of Foxp3 T reg cells via retinoic acid. JEM 204: 1775-1785 [Abstract] [Full Text]  
• Benson, M. J., Pino-Lagos, K., Rosemblatt, M., Noelle, R. J. (2007). All-trans retinoic acid mediates enhanced T reg cell growth, differentiation, and gut homing in the face of high levels of co-stimulation. JEM 204: 1765-1774 [Abstract] [Full Text]  
• Takenaka, S., Safroneeva, E., Xing, Z., Gauldie, J. (2007). Dendritic Cells Derived from Murine Colonic Mucosa Have Unique Functional and Phenotypic Characteristics. J. Immunol. 178: 7984-7993 [Abstract] [Full Text]  
• Stenstad, H., Svensson, M., Cucak, H., Kotarsky, K., Agace, W. W. (2007). Differential homing mechanisms regulate regionalized effector CD8{alpha}beta+ T cell accumulation within the small intestine. Proc. Natl. Acad. Sci. USA 104: 10122-10127 [Abstract] [Full Text]  
• del Rio, M.-L., Rodriguez-Barbosa, J.-I., Kremmer, E., Forster, R. (2007). CD103- and CD103+ Bronchial Lymph Node Dendritic Cells Are Specialized in Presenting and Cross-Presenting Innocuous Antigen to CD4+ and CD8+ T Cells. J. Immunol. 178: 6861-6866 [Abstract] [Full Text]  
• Saalbach, A., Klein, C., Sleeman, J., Sack, U., Kauer, F., Gebhardt, C., Averbeck, M., Anderegg, U., Simon, J. C. (2007). Dermal Fibroblasts Induce Maturation of Dendritic Cells. J. Immunol. 178: 4966-4974 [Abstract] [Full Text]  
• Kiriya, K., Watanabe, N., Nishio, A., Okazaki, K., Kido, M., Saga, K., Tanaka, J., Akamatsu, T., Ohashi, S., Asada, M., Fukui, T., Chiba, T. (2007). Essential role of Peyer's patches in the development of Helicobacter-induced gastritis. Int Immunol 19: 435-446 [Abstract] [Full Text]  
• Marski, M., Ye, A. L., Abraham, C. (2007). CD18 Is Required for Intestinal T Cell Responses at Multiple Immune Checkpoints. J. Immunol. 178: 2104-2112 [Abstract] [Full Text]  
• Beaty, S. R., Rose, C. E. Jr., Sung, S.-s. J. (2007). Diverse and Potent Chemokine Production by Lung CD11bhigh Dendritic Cells in Homeostasis and in Allergic Lung Inflammation. J. Immunol. 178: 1882-1895 [Abstract] [Full Text]  
• Zhang, C., Lou, J., Li, N., Todorov, I., Lin, C.-L., Cao, Y.-A., Contag, C. H., Kandeel, F., Forman, S., Zeng, D. (2007). Donor CD8+ T Cells Mediate Graft-versus-Leukemia Activity without Clinical Signs of Graft-versus-Host Disease in Recipients Conditioned with Anti-CD3 Monoclonal Antibody. J. Immunol. 178: 838-850 [Abstract] [Full Text]  
• Klonowski, K. D., Marzo, A. L., Williams, K. J., Lee, S.-J., Pham, Q.-M., Lefrancois, L. (2006). CD8 T Cell Recall Responses Are Regulated by the Tissue Tropism of the Memory Cell and Pathogen. J. Immunol. 177: 6738-6746 [Abstract] [Full Text]  
• Hintzen, G., Ohl, L., del Rio, M.-L., Rodriguez-Barbosa, J.-I., Pabst, O., Kocks, J. R., Krege, J., Hardtke, S., Forster, R. (2006). Induction of Tolerance to Innocuous Inhaled Antigen Relies on a CCR7-Dependent Dendritic Cell-Mediated Antigen Transport to the Bronchial Lymph Node. J. Immunol. 177: 7346-7354 [Abstract] [Full Text]  
• Kwa, S.-f., Beverley, P., Smith, A. L. (2006). Peyer's Patches Are Required for the Induction of Rapid Th1 Responses in the Gut and Mesenteric Lymph Nodes during an Enteric Infection.. J. Immunol. 176: 7533-7541 [Abstract] [Full Text]  
• Worbs, T., Bode, U., Yan, S., Hoffmann, M. W., Hintzen, G., Bernhardt, G., Forster, R., Pabst, O. (2006). Oral tolerance originates in the intestinal immune system and relies on antigen carriage by dendritic cells. JEM 203: 519-527 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search

TABLE OF CONTENTS