The Journal of Experimental Medicine
for flow cytometry > invitrogen
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online 26 September 2005 doi:10.1084/jem.20050463
Rockefeller University Press, 0022-1007 $8.00
JEM, Volume 202, Number 7, 919-929
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 1504K)
Right arrow PPT slides of all figures
Right arrow Supplemental Material Index
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ghiringhelli, F.
Right arrow Articles by Zitvogel, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ghiringhelli, F.
Right arrow Articles by Zitvogel, L.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

ARTICLE

 Tumor cells convert immature myeloid dendritic cells into TGF-ß–secreting cells inducing CD4+CD25+ regulatory T cell proliferation

François Ghiringhelli1, Pierre E. Puig1, Stephan Roux2, Arnaud Parcellier1, Elise Schmitt1, Eric Solary1, Guido Kroemer3, François Martin1, Bruno Chauffert1, and Laurence Zitvogel2

1 Institut National de la Santé et de la Recherche Médicale, U517, University of Burgundy, 21079 Dijon, France
2 ERM 0208, Institut National de la Santé et de la Recherche Médicale
3 UMR 8125, Centre National de la Recherche Scientifique, Institut Gustave Roussy, 94805 Villejuif, France

CORRESPONDENCE Laurence Zitvogel: zitvogel{at}igr.fr OR Bruno Chauffert: Bchauffert{at}dijon.fnclcc.fr

The mechanisms through which regulatory T cells accumulate in lymphoid organs of tumor-bearing hosts remain elusive. Our experiments indicate that the accumulation of CD4+CD25+ regulatory T cells (T reg cells) expressing FoxP3 and exhibiting immunosuppressive function originates from the proliferation of naturally occurring CD25+ T cells and requires signaling through transforming growth factor (TGF)–ß receptor II. During tumor progression, a subset of dendritic cells (DCs) exhibiting a myeloid immature phenotype is recruited to draining lymph nodes. This DC subset selectively promotes the proliferation of T reg cells in a TGF-ß–dependent manner in mice and rats. Tumor cells are necessary and sufficient to convert DCs into regulatory cells that secrete bioactive TGF-ß and stimulate T reg cell proliferation. In conclusion, tumor expansion can stimulate T reg cells via a specific DC subset.

Abbreviations used: BrdU, 5-bromo-2'-deoxyuridine; CFSE, carboxyfluorescein diacetate succinimidyl ester; ConA, concanavalin A; DLN, draining LN; DN TGF-ßRII mice, dominant-negative TGF-ß receptor II–signaling mice bearing transgenic T cells; IMDC, immature myeloid DC; TBM, tumor-bearing mice; TBR, tumor-bearing rats; TFM, tumor-free mouse/mice; TFR, tumor-free rats; T reg cells, CD4+CD25+ regulatory T cells.

B. Chauffert and L. Zitvogel contributed equally to this work.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS