The Journal of Experimental Medicine
Torrey Pines Biolabs
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published 15 August 2005. doi:10.1084/jem.20050381
Rockefeller University Press, 0022-1007 $8.00
JEM, Volume 202, Number 4, 479-484
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 1011K)
Right arrow PPT slides of all figures
Right arrow Supplemental Material Index
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wheat, W. H.
Right arrow Articles by Routes, J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wheat, W. H.
Right arrow Articles by Routes, J. M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

BRIEF DEFINITIVE REPORT

 Possible role of human herpesvirus 8 in the lymphoproliferative disorders in common variable immunodeficiency

William H. Wheat1, Carlyne D. Cool2,7, Yoshikazu Morimoto7, Pradeep R. Rai2, Charles H. Kirkpatrick3, Barbara A. Lindenbaum7, Christopher A. Bates7, Misoo C. Ellison4,5,7, Amanda E. Serls2, Kevin K. Brown3,7, and John M. Routes1,3,6,7

1 Integrated Department of Immunology, National Jewish Medical and Research Center and University of Colorado Health Sciences Center
2 Department of Pathology, University of Colorado Health Sciences Center, Denver, CO 80262
3 Department of Medicine, University of Colorado Health Sciences Center, Denver, CO 80262
4 Department of Preventive Medicine, University of Colorado Health Sciences Center, Denver, CO 80262
5 Department of Biometrics, University of Colorado Health Sciences Center, Denver, CO 80262
6 Cancer Center, University of Colorado Health Sciences Center, Denver, CO 80262
7 Department of Medicine, National Jewish Medical and Research Center, Denver, CO 80206

CORRESPONDENCE John M. Routes: routesj{at}njc.org

Patients who have common variable immunodeficiency (CVID) and granulomatous/lymphocytic interstitial lung disease (GLILD) are at high risk for early mortality and B cell lymphomas. Infection with human herpes virus type 8 (HHV8), a B cell lymphotrophic virus, is linked to lymphoproliferative disorders in people who have secondary immunodeficiencies. Therefore, we determined the prevalence of HHV8 infection in CVID patients with GLILD. Genomic DNA isolated from peripheral blood mononuclear cells was screened by nested- and real time-quantitative PCR (QRT-PCR) for the presence of HHV8 genome. It was positive in 6/9 CVID patients with GLILD (CVID-GLILD), 1/21 CVID patients without GLILD (CVID-control), and no patients receiving intravenous gamma globulin (n = 13) or normal blood donors (n = 20). Immunohistochemistry (IHC) demonstrated expression of the latency-associated nuclear antigen-1 (LANA-1) in the biopsies of the lung, liver, and bone marrow of four patients with CVID-GLILD. One CVID-GLILD patient developed a B cell lymphoma during the course of the study. QRT-PCR demonstrated high copy number of HHV8 genome and IHC showed diffuse staining for LANA-1 in the malignant lymph node. HHV8 infection may be an important factor in the pathogenesis of the interstitial lung disease and lymphoproliferative disorders in patients with CVID.

W.H. Wheat's present address is Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, CO 80523.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS