The Journal of Experimental Medicine
Fluorescence In Vivo Endomicroscopy
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Published 18 July 2005. doi:10.1084/jem.20050569
Rockefeller University Press, 0022-1007 $8.00
JEM, Volume 202, Number 2, 309-319
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ARTICLE

 CD4–CD8– T cells control intracellular bacterial infections both in vitro and in vivo

Siobhán C. Cowley1, Elizabeth Hamilton1, Jeffrey A. Frelinger2, Jie Su3, James Forman3, and Karen L. Elkins1

1 Laboratory of Mycobacterial Diseases and Cellular Immunology, Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, Rockville, MD 20852
2 Department of Microbiology and Immunology, University of North Carolina, Chapel Hill, Chapel Hill, NC 27599
3 Center for Immunology, University of Texas Southwestern Medical Center, Dallas, TX 75390

CORRESPONDENCE Siobhán C. Cowley: cowley{at}cber.fda.gov OR Karen L. Elkins: elkins{at}cber.fda.gov

Memory T cells, including the well-known CD4+ and CD8+ T cells, are central components of the acquired immune system and are the basis for successful vaccination. After infection, CD4+ and CD8+ T cells expand into effector cells, and then differentiate into long-lived memory cells. We show that a rare population of CD4–CD8–CD3+{alpha}ß+{gamma}{delta}–NK1.1– T cells has similar functions. These cells potently and specifically inhibit the growth of the intracellular bacteria Mycobacterium tuberculosis (M. tb.) or Francisella tularensis Live Vaccine Strain (LVS) in macrophages in vitro, promote survival of mice infected with these organisms in vivo, and adoptively transfer immunity to F. tularensis LVS. Furthermore, these cells expand in the spleens of mice infected with M. tb. or F. tularensis LVS, and then acquire a memory cell phenotype. Thus, CD4–CD8– T cells have a role in the control of intracellular infection and may contribute to successful vaccination.

Abbreviations used: BMMØ, BM-derived macrophage; DN, double negative; i.d., intradermal(ly); LVS, Live Vaccine Strain; MOI, multiplicity of infection; M. tb., Mycobacterium tuberculosis.


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