The Journal of Experimental Medicine
Rockland Immunochemicals for Research
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Published online 25 April 2005 doi:10.1084/jem.20041204
Rockefeller University Press, 0022-1007 $8.00
JEM, Volume 201, Number 9, 1447-1457
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ARTICLE

 Differential requirements for the chemokine receptor CCR7 in T cell activation during Listeria monocytogenes infection

Mischo Kursar1, Uta E. Höpken2, Markus Koch1, Anne Köhler1, Martin Lipp2, Stefan H.E. Kaufmann1, and Hans-Willi Mittrücker1

1 Department of Immunology, Max-Planck Institute for Infection Biology, 10117 Berlin, Germany
2 Department for Molecular Tumor Genetics and Immunogenetics, Max-Delbrück Center for Molecular Medicine, 13092 Berlin, Germany

CORRESPONDENCE Hans-Willi Mittrücker: mittruecker{at}mpiib-berlin.mpg.de

Effective priming of T cell responses depends on cognate interactions between naive T cells and professional antigen-presenting cells (APCs). This contact is the result of highly coordinated migration processes, in which the chemokine receptor CCR7 and its ligands, CCL19 and CCL21, play a central role. We used the murine Listeria monocytogenes infection model to characterize the role of the CCR7/CCR7 ligand system in the generation of T cell responses during bacterial infection. We demonstrate that efficient priming of naive major histocompatibility complex (MHC) class Ia–restricted CD8+ T cells requires CCR7. In contrast, MHC class Ib–restricted CD8+ T cells and MHC class II–restricted CD4+ T cells seem to be less dependent on CCR7; memory T cell responses are independent of CCR7. Infection experiments with bone marrow chimeras or mice reconstituted with purified T cell populations indicate that CCR7 has to be expressed on CD8+ T cells and professional APCs to promote efficient MHC class Ia–restricted T cell priming. Thus, different T cell subtypes and maturation stages have discrete requirements for CCR7.

Abbreviations used: CFSE, carboxyfluorescein succinimidyl ester; f-met, formyl-methionine; LLO, listeriolysin O; LmOVA, recombinant Listeria monocytogenes strain secreting a truncated OVA protein; MACS, magnetic cell sorting; TSB, tryptic soy broth.


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