The Journal of Experimental Medicine
for flow cytometry > invitrogen
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Published 20 June 2005. doi:10.1084/jem.20042408
Rockefeller University Press, 0022-1007 $8.00
JEM, Volume 201, Number 12, 1999-2010
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ARTICLE

 Repertoire, diversity, and differentiation of specific CD8 T cells are associated with immune protection against human cytomegalovirus disease

Karim Sacre1, Guislaine Carcelain1, Nathalie Cassoux2, Anne-Marie Fillet3, Dominique Costagliola4, Daniel Vittecoq7, Dominique Salmon8, Zahir Amoura5, Christine Katlama6, and Brigitte Autran1

1 Laboratoire d'Immunologie Cellulaire et Tissulaire, INSERM U543
2 Service d'Ophtalmologie, EA 2387
3 Laboratoire de Virologie, EA 2387
4 Unité d'Epidémiologie Clinique et Traitements de l'Infection VIH, INSERM U720
5 Service de Médecine Interne, Hôpital Pitié-Salpétrière, Université Pierre et Marie Curie, 75013 Paris, France
6 Service des Maladies Infectieuses, Hôpital Pitié-Salpétrière, Université Pierre et Marie Curie, 75013 Paris, France
7 Service des Maladies Infectieuses, Hôpital Paul Brousse, 94800 Villejuif, France
8 Service de Médecine Interne, Hôpital Cochin, 75014 Paris, France

CORRESPONDENCE Brigitte Autran: brigitte.autran{at}psl.ap-hop-paris.fr

To determine the correlates of immune recovery from active human CMV (HCMV) disease, we compared the antigenic repertoire, diversity, magnitude, and differentiation of HCMV-specific CD8+ T cells in HIV-HCMV coinfected subjects with no, cured, or active HCMV disease and in healthy HIV-negative HCMV-positive controls. ELISPOT–IFN-{gamma} assays using peptide pools spanning the pp65 and immediate early 1 (IE1) HCMV proteins showed that HCMV-specific CD8+ T cells had a significantly broader antigenic repertoire and greater diversity in HIV-positive patients controlling HCMV replication than in those with active HCMV disease, but the magnitude of the CD8 T cell response did not differ between the different groups. HCMV-specific T cells mainly were focused against IE1 during the short-term recovery from retinitis, and switched toward pp65 during long-term recovery. HCMV-specific T cells displaying an "early" (CD8+CD27+CD28+) and "intermediate" (CD8+CD27–CD28+) differentiation phenotype were increased significantly during long-term recovery compared with other HIV-positive patients and were nearly undetectable during active HCMV disease. HCMV-specific T cells with a "late" (CD8+CD27–28–) differentiation phenotype predominated in all cases. Therefore, restoration of immune protection against HCMV after active HCMV disease in immunodeficient individuals is associated with enlarged repertoire and diversity, and with early differentiation of virus-specific CD8+ T cells, thus defining immune correlates of protection against diseases caused by persistent viruses.

Abbreviations used: AHCMV, active human CMV; HAART, highly active antiretroviral therapy; HCMV, human CMV; IE1, immediate early 1; LTNP, long-term nonprogressor; LTRR, long-term recovery from retinitis; SFC, spot-forming cell; STRR, short-term recovery from retinitis.


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