The Journal of Experimental Medicine
Avanti Polar Lipids, Inc.
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online 31 May 2005 doi:10.1084/jem.20042524
Rockefeller University Press, 0022-1007 $8.00
JEM, Volume 201, Number 11, 1715-1723
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 3883K)
Right arrow PPT slides of all figures
Right arrow Supplemental Material Index
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dik, W. A.
Right arrow Articles by Staal, F. J.T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dik, W. A.
Right arrow Articles by Staal, F. J.T.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

BRIEF DEFINITIVE REPORT

 New insights on human T cell development by quantitative T cell receptor gene rearrangement studies and gene expression profiling

Willem A. Dik1, Karin Pike-Overzet1, Floor Weerkamp1, Dick de Ridder1,3, Edwin F.E. de Haas1, Miranda R.M. Baert1, Peter van der Spek2, Esther E.L. Koster1, Marcel J.T. Reinders3, Jacques J.M. van Dongen1, Anton W. Langerak1, and Frank J.T. Staal1

1 Department of Immunology, Erasmus MC, 3015 GE Rotterdam, Netherlands
2 Department of Bioinformatics, Erasmus MC, 3015 GE Rotterdam, Netherlands
3 Information and Communication Theory Group, Faculty of Electrical Engineering, Mathematics and Computer Science, Delft University of Technology, 2600 GA Delft, Netherlands

CORRESPONDENCE Frank J.T. Staal: f.staal{at}erasmusmc.nl

To gain more insight into initiation and regulation of T cell receptor (TCR) gene rearrangement during human T cell development, we analyzed TCR gene rearrangements by quantitative PCR analysis in nine consecutive T cell developmental stages, including CD34+ lin– cord blood cells as a reference. The same stages were used for gene expression profiling using DNA microarrays. We show that TCR loci rearrange in a highly ordered way (TCRD-TCRG-TCRB-TCRA) and that the initiating D{delta}2-D{delta}3 rearrangement occurs at the most immature CD34+CD38–CD1a– stage. TCRB rearrangement starts at the CD34+CD38+CD1a– stage and complete in-frame TCRB rearrangements were first detected in the immature single positive stage. TCRB rearrangement data together with the PTCRA (pT{alpha}) expression pattern show that human TCRß-selection occurs at the CD34+CD38+CD1a+ stage. By combining the TCR rearrangement data with gene expression data, we identified candidate factors for the initiation/regulation of TCR recombination. Our data demonstrate that a number of key events occur earlier than assumed previously; therefore, human T cell development is much more similar to murine T cell development than reported before.

W.A. Dik and K. Pike-Overzet contributed equally to this work.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS