The Journal of Experimental Medicine
Aegean Conferences: 2009 Conferences
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Published 16 May 2005. doi:10.1084/jem.20050030
Rockefeller University Press, 0022-1007 $8.00
JEM, Volume 201, Number 10, 1579-1589
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ARTICLE

 Hematopoietic cells maintain hematopoietic fates upon entering the brain

Mei Massengale1, Amy J. Wagers1, Hannes Vogel1, and Irving L. Weissman1,2

1 Department of Pathology, Stanford University School of Medicine, Stanford, CA 94305
2 Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305

CORRESPONDENCE Amy J. Wagers: amy.wagers{at}joslin.harvard.edu

Several studies have reported that bone marrow (BM) cells may give rise to neurons and astrocytes in vitro and in vivo. To further test this hypothesis, we analyzed for incorporation of neural cell types expressing donor markers in normal or injured brains of irradiated mice reconstituted with whole BM or single, purified c-kit+Thy1.1loLin–Sca-1+ (KTLS) hematopoietic stem cells (HSCs), and of unirradiated parabionts with surgically anastomosed vasculature. Each model showed low-level parenchymal engraftment of donor-marker+ cells with 96–100% immunoreactivity for panhematopoietic (CD45) or microglial (Iba1 or Mac1) lineage markers in all cases studied. Other than one arborizing structure in the olfactory bulb of one BM-transplanted animal, possibly representing a neuronal or glial cell process, we found no donor-marker–expressing astrocytes or non-Purkinje neurons among >10,000 donor-marker+ cells from 21 animals. These data strongly suggest that HSCs and their progeny maintain lineage fidelity in the brain and do not adopt neural cell fates with any measurable frequency.

Abbreviations used: CNS, central nervous system; EGFP, enhanced GFP; GFAP, glial fibrillary acidic protein; HSC, hematopoietic stem cell; KA, kainic acid; KTLS, c-kit+Thy1.1loLin–Sca-1+; PB, peripheral blood; WBM, whole BM.

A.J. Wager's present address is Section on Developmental and Stem Cell Biology, Joslin Diabetes Center, Boston, MA 02215.


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