The Journal of Experimental Medicine
Torrey Pines Biolabs
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published 4 October 2004. doi:10.1084/jem.20040598
Rockefeller University Press, 0022-1007 $8.00
JEM, Volume 200, Number 7, 857-870
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 360K)
Right arrow PPT slides of all figures
Right arrow Supplemental Material Index
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Khodoun, M. V.
Right arrow Articles by Finkelman, F. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Khodoun, M. V.
Right arrow Articles by Finkelman, F. D.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?
Basophils Initiate IL-4 Production during a Memory T-dependent Response

Marat V. Khodoun, Tatyana Orekhova1, Crystal Potter1, Suzanne Morris1,2, and Fred D. Finkelman1,2,3

1 Department of Medicine, University of Cincinnati College of Medicine, Cincinnati, OH 45267
2 Cincinnati Veterans Affairs Medical Center, Cincinnati, OH 45220
3 Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229

Address correspondence to Fred Finkelman, Dept. of Medicine, University of Cincinnati College of Medicine, Cincinnati, OH 45267. Phone: (513) 558-4701; Fax: (513) 558-3799; email: ffinkelman{at}pol.net

Experiments were performed to characterize and identify the cellular sources of the secondary interleukin (IL)-4 response to a T cell–dependent antigen. Mice were primed by immunization with goat anti–mouse immunoglobulin (Ig)D antibody (GaMD), which stimulates naive CD4+ T cells to secrete IL-4 in 3–4 d. When challenged with goat serum 14 d after immunization, GaMD-primed mice generated an IL-4 response that exceeded the primary response by ~100-fold, started in <2 h, and lasted for 4 d. Studies with 4get mice, in which cells with an accessible Il4 gene express a green fluorescent protein (GFP), revealed CD4+ memory T cells, natural killer T cells, basophils, mast cells, and eosinophils as possible rapid producers of IL-4. GFP+CD4+ T cells and basophils expanded more in the spleen than the other cell types during the primary response to GaMD. Quantitation of in vivo IL-4 production by the in vivo cytokine capture assay after individual cell types were selectively stimulated or deleted demonstrated that basophils and memory CD4+ T cells account for most of the secondary IL-4 response, with basophils initiating that response through IgE/Fc{varepsilon}RI-mediated signaling but secreting IL-4 for <4 h and memory T cells secreting IL-4 within 4 h and continuing to secrete this cytokine for 4 d.

Key Words: cytokine • allergy • NKT cell • eosinophil • mast cell

Abbreviations used in this paper: GaMD, goat anti–mouse IgD antibody; IVCCA, in vivo cytokine capture assay; MMCP1, mouse mast cell protease 1.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS