The Journal of Experimental Medicine
Fluorescence In Vivo Endomicroscopy
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online 9 August 2004 doi:10.1084/jem.20040383
Rockefeller University Press, 0022-1007 $8.00
JEM, Volume 200, Number 4, 481-491
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 2804K)
Right arrow PPT slides of all figures
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Misslitz, A.
Right arrow Articles by Förster, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Misslitz, A.
Right arrow Articles by Förster, R.
Right arrowPubmed/NCBI databases
*Gene*GEO Profiles
*HomoloGene*UniGene
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Thymic T Cell Development and Progenitor Localization Depend on CCR7

Ana Misslitz1, Oliver Pabst1, Gabriele Hintzen1, Lars Ohl1, Elisabeth Kremmer2, Howard T. Petrie3, and Reinhold Förster1

1 Institute of Immunology, Hannover Medical School, 30625 Hannover, Germany
2 Institute of Molecular Immunology, GSF, 81377 Munich, Germany
3 University of Miami, School of Medicine, Miami, FL 33101

Address correspondence to Reinhold Förster, Institute of Immunology, Hannover Medical School, Carl-Neuberg-Str. 1, Bldg. K11, Level 2, 30625 Hannover, Germany. Phone: 49-511-5329721; Fax: 49-511-5329722; email: foerster.reinhold{at}mh-hannover.de

T cell differentiation in the adult thymus depends on sequential interactions between lymphoid progenitors and stromal cells found in distinct regions of the cortex and medulla. Therefore, migration of T cell progenitors through distinct stromal environments seems to be a crucial process regulating differentiation and homeostasis inside the thymus.

Here we show that CCR7-deficient mice are distinguished by a disturbed thymic architecture, impaired T cell development, and decreased numbers of the thymocytes. Analysis of developing double negative (CD4–CD8–) pool of wild-type thymus reveals that CCR7 expression is restricted to a CD25intCD44+ subpopulation. Correspondingly, CCR7 deficiency results in an accumulation of this population in mutant thymus. Furthermore, immunohistology shows that in CCR7-deficient mice CD25+CD44+ cells accumulate at the cortico-medullary junction, suggesting that CCR7 signaling regulates the migration of early progenitors toward the outer thymic cortex, thereby continuing differentiation. Results obtained from mixed bone marrow chimeras support this view, since the development of CCR7-deficient thymocytes is also disturbed in a morphologically intact thymus. Thus, our findings establish an essential role for CCR7 in intrathymic migration and proper T cell development.

Key Words: chemokines • T cell development • cell migration • thymus • progenitor

A. Misslitz and O. Pabst contributed equally to this work.

Abbreviations used in this paper: CMJ, cortico-medullary junction; DN, double negative; DP, double positive; SCZ, subcapsular zone; SP, single positive.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS