The Journal of Experimental Medicine
Avanti Polar Lipids, Inc.
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online 12 July 2004 doi:10.1084/jem.20032082
Rockefeller University Press, 0022-1007 $8.00
JEM, Volume 200, Number 2, 137-147
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 422K)
Right arrow PPT slides of all figures
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Stokes, A. J.
Right arrow Articles by Turner, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Stokes, A. J.
Right arrow Articles by Turner, H.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?
A TRPV2–PKA Signaling Module for Transduction of Physical Stimuli in Mast Cells

Alexander J. Stokes1, Lori M.N. Shimoda1, Murielle Koblan-Huberson1, Chaker N. Adra2, and Helen Turner1,3

1 Center for Biomedical Research at the Queen's Medical Center, Honolulu, HI 96813
2 Beth Israel Deaconess Medical Center, Boston, MA 02215
3 Department of Cell and Molecular Biology, University of Hawaii, Honolulu, HI 96822

Address correspondence to Helen Turner, Center for Biomedical Research, 1301 Punchbowl St., University Tower 8, Honolulu, HI 96813. Phone: (808) 537-7927; Fax: (808) 537-7926; email: hturner{at}queens.org

Cutaneous mast cell responses to physical (thermal, mechanical, or osmotic) stimuli underlie the pathology of physical urticarias. In vitro experiments suggest that mast cells respond directly to these stimuli, implying that a signaling mechanism couples functional responses to physical inputs in mast cells. We asked whether transient receptor potential (vanilloid) (TRPV) cation channels were present and functionally coupled to signaling pathways in mast cells, since expression of this channel subfamily confers sensitivity to thermal, osmotic, and pressure inputs. Transcripts for a range of TRPVs were detected in mast cells, and we report the expression, surface localization, and oligomerization of TRPV2 protein subunits in these cells. We describe the functional coupling of TRPV2 protein to calcium fluxes and proinflammatory degranulation events in mast cells. In addition, we describe a novel protein kinase A (PKA)–dependent signaling module, containing PKA and a putative A kinase adapter protein, Acyl CoA binding domain protein (ACBD)3, that interacts with TRPV2 in mast cells. We propose that regulated phosphorylation by PKA may be a common pathway for TRPV modulation.

Key Words: calcium channels • inflammation • physical urticaria • transient receptor potential • vanilloid receptor

Abbreviations used in this paper: ACBD, Acyl CoA binding domain protein; AKAP, A kinase adapter protein; BMMC, BM-derived mast cell; PKA, protein kinase A; PKAR, PKA regulatory; PKG, protein kinase G; PU, physical urticaria; TRPV, transient receptor potential (vanilloid); VDAC, voltage-dependent anion channel.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS