The Journal of Experimental Medicine
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Published 17 February 2004. doi:10.1084/jem.20030437
Rockefeller University Press, 0022-1007 $8.00
JEM, Volume 199, Number 4, 471-482
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Specific Regulation of T Helper Cell 1–mediated Murine Colitis by CEACAM1

Hideki Iijima1, Markus F. Neurath1,3, Takashi Nagaishi1, Jonathan N. Glickman2, Edward E. Nieuwenhuis1, Atsushi Nakajima4, Daohong Chen1, Ivan J. Fuss5, Nalan Utku6, Daniel N. Lewicki7, Christoph Becker3, Thomas M. Gallagher7, Kathryn V. Holmes8, and Richard S. Blumberg1

1 Gastroenterology Division, Department of Medicine
2 Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115
3 Laboratory of Immunology, I. Medical Clinic, University of Mainz, Mainz 55128, Germany
4 The Third Department of Internal Medicine, Yokohama City University School of Medicine, Yokohama 236-0004, Japan
5 Mucosal Immunity Section, Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892
6 Department of Immunology, Charite-Humboldt University, Berlin 10115, Germany
7 Department of Microbiology and Immunology, Loyola University Medical Center, Maywood, IL 60153
8 Department of Microbiology, University of Colorado Health Sciences Center, Denver, CO 80262

Address correspondence to Richard S. Blumberg, Gastroenterology Division, Brigham and Women's Hospital, Harvard Medical School, 75 Francis Street, Boston, MA 02115. Phone: (617) 732-6917; Fax: (617) 264-5185; email: rblumberg{at}partners.org

Carcinoembryonic antigen-related cellular adhesion molecule 1 (CEACAM1) is a cell surface molecule that has been proposed to negatively regulate T cell function. We have shown that CEACAM1 is associated with specific regulation of T helper cell (Th)1 pathways, T-bet–mediated Th1 cytokine signaling, and Th1-mediated immunopathology in vivo. Mice treated with anti–mouse CEACAM1-specific monoclonal antibody (mAb) CC1 during the effector phase exhibited a reduced severity of trinitrobenzene sulfonic acid colitis in association with decreased interferon (IFN)-{gamma} production. Although oxazolone colitis has been reported as Th2 mediated, mice treated with the CC1 mAb or a CEACAM1-Fc chimeric protein exhibited a reduced severity of colitis in association with a significant reduction of IFN-{gamma} and T-bet activation, whereas signal transducer and activator of antigen 4 activation was unaffected. Both interleukin-4 and IFN-{gamma} gene–deficient mice exhibited less severe colitis induction by oxazolone. Direct ligation of T cells in vitro with the murine hepatitis virus spike protein, a natural ligand for the N-domain of CEACAM1, inhibited the differentiation of naive cells into Th1 but not Th2 cells and activation of Th1 but not Th2 cytokine production. These results indicate that CEACAM1 isoforms are a novel class of activation-induced cell surface molecules on T cells that function in the specific regulation of Th1-mediated inflammation such as that associated with inflammatory bowel disease.

Key Words: CEACAM1 • inflammatory bowel disease • hapten-induced colitis • T cell immunity • Th1 cytokine

The online version of this article contains supplemental material.

Abbreviations used in this paper: CEACAM1, carcinoembryonic antigen-related cellular adhesion molecule 1; ITIM, immunoreceptor tyrosine-based inhibitory motif; LPL, lamina propria lymphocyte; MHV, mouse hepatitis virus; STAT-4, signal transducer and activator of antigen 4; TNBS, trinitrobenzene sulfonic acid.


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