The Journal of Experimental Medicine
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Published online 12 January 2004 doi:10.1084/jem.20031615
Rockefeller University Press, 0022-1007 $8.00
JEM, Volume 199, Number 2, 221-229
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ß-Catenin Is Dispensable for Hematopoiesis and Lymphopoiesis

Monica Cobas1, Anne Wilson1, Bettina Ernst1, Stéphane J.C. Mancini1, H. Robson MacDonald1, Rolf Kemler2, and Freddy Radtke1

1 Ludwig Institute for Cancer Research, Lausanne Branch, University of Lausanne, 1066 Epalinges, Switzerland
2 Department of Molecular Embryology, Max-Planck Institute of Immunology, 79108 Freiburg, Germany

Address correspondence to Freddy Radtke, Ludwig Institute for Cancer Research, Lausanne Branch, University of Lausanne, Chemin des Boveresses 155, 1066 Epalinges, Switzerland. Phone: 41-21-692-59-64; Fax: 41-21-653-44-74; email: Freddy.Radtke{at}isrec.unil.ch

ß-catenin–mediated Wnt signaling has been suggested to be critically involved in hematopoietic stem cell maintenance and development of T and B cells in the immune system. Unexpectedly, here we report that inducible Cre-loxP–mediated inactivation of the ß-catenin gene in bone marrow progenitors does not impair their ability to self-renew and reconstitute all hematopoietic lineages (myeloid, erythroid, and lymphoid), even in competitive mixed chimeras. In addition, both thymocyte survival and antigen-induced proliferation of peripheral T cells is ß-catenin independent. In contrast to earlier reports, these data exclude an essential role for ß-catenin during hematopoiesis and lymphopoiesis.

Key Words: Wnt signaling • T cells • B cells • development • gene targeting

Abbreviations used in this paper: APC, adenomatous polyposis coli; CFSE, 5- and 6-carboxyfluorescein diacetate succinimidyl ester; CLP, common lymphoid progenitor; CMP, common myeloid progenitor; DN, double negative; DP, double positive; FSC, forward scatter; HSC, hematopoietic stem cell; ISP, immature single positive; pI-pC, polyI-polyC; SEB, staphylococcal enterotoxin B; SSC, side scatter.


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