The Journal of Experimental Medicine
IL-17 System from Mabtech
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online 29 December 2003 doi:10.1084/jem.20031237
Rockefeller University Press, 0022-1007 $8.00
JEM, Volume 199, Number 1, 81-90
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 397K)
Right arrow PPT slides of all figures
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Doyle, S. E.
Right arrow Articles by Cheng, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Doyle, S. E.
Right arrow Articles by Cheng, G.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Toll-like Receptors Induce a Phagocytic Gene Program through p38

Sean E. Doyle1, Ryan M. O'Connell1, Gustavo A. Miranda2,3, Sagar A. Vaidya1,4, Edward K. Chow5, Philip T. Liu1, Shinobu Suzuki8, Nobutaka Suzuki8, Robert L. Modlin6, Wen-Chen Yeh8, Timothy F. Lane2,3 and Genhong Cheng1,2,7

1 Department of Microbiology, Immunology and Molecular Genetics
2 Jonsson Comprehensive Cancer Center, Department of Medicine, David Geffen School of Medicine
3 Department of Obstetrics and Gynecology and Biological Chemistry, Department of Medicine, David Geffen School of Medicine
4 Medical Scientist Training Program Graduate Program, Department of Medicine, David Geffen School of Medicine
5 UCLA ACCESS Graduate Program, Department of Medicine, David Geffen School of Medicine
6 Division of Dermatology, Department of Medicine, David Geffen School of Medicine
7 Molecular Biology Institute, University of California, Los Angeles, Los Angeles, CA 90095
8 Advanced Medical Discovery Institute, University Health Network and Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada M5G 2C1

Address correspondence to Genhong Cheng, University of California, Los Angeles, Dept. of Microbiology, Immunology and Molecular Genetics, 8-240 Factor Building, 10833 Le Conte Avenue, Los Angeles, CA 90095. Phone: (310) 825-8896; Fax: (310) 206-5553; email: genhongc{at}microbio.ucla.edu

Toll-like receptor (TLR) signaling and phagocytosis are hallmarks of macrophage-mediated innate immune responses to bacterial infection. However, the relationship between these two processes is not well established. Our data indicate that TLR ligands specifically promote bacterial phagocytosis, in both murine and human cells, through induction of a phagocytic gene program. Importantly, TLR-induced phagocytosis of bacteria was found to be reliant on myeloid differentiation factor 88–dependent signaling through interleukin-1 receptor–associated kinase-4 and p38 leading to the up-regulation of scavenger receptors. Interestingly, individual TLRs promote phagocytosis to varying degrees with TLR9 being the strongest and TLR3 being the weakest inducer of this process. We also demonstrate that TLR ligands not only amplify the percentage of phagocytes uptaking Escherichia coli, but also increase the number of bacteria phagocytosed by individual macrophages. Taken together, our data describe an evolutionarily conserved mechanism by which TLRs can specifically promote phagocytic clearance of bacteria during infection.

Key Words: macrophage • phagocytosis • toll-like receptor • scavenger receptor • p38

Sean E. Doyle and Ryan M. O'Connell contributed equally to this work.

Abbreviations used in this paper: BMM, bone marrow–derived macrophage; DAPI, 6'-diamidino-2-phenylindole; ERK 1/2, extracellular signal–related kinase 1/2; FISH, fluorescence in situ hybridization; GFP, green fluorescence protein; ICAM-1, intercellular adhesion molecule-1; IRAK, IL-1 receptor–associated kinase; LOX-1, lectin-like oxidized LDL receptor; MARCO, macrophage scavenger receptor with collagenous structure; M-CSF, macrophage colony-stimulating factor; MOI, multiplicity of infection; MyD88, myeloid differentiation factor 88; NF, nuclear factor; PGN, peptidoglycan; poly I, polyinosinic acid; PRR, pattern recognition receptor; Q-PCR, quantitative realtime PCR; SR, scavenger receptor; SR-A, scavenger receptor-A; TLR, toll-like receptor; TRAF6, tumor necrosis factor receptor–associated factor 6.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS