The Journal of Experimental Medicine
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Published online 27 October 2003 doi:10.1084/jem.20030916
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© Rockefeller University Press, 0022-1007/2003/11/1369 $5.00
The Journal of Experimental Medicine, Volume 198, Number 9, 1369-1380

CD27 Promotes Survival of Activated T Cells and Complements CD28 in Generation and Establishment of the Effector T Cell Pool

Jenny Hendriks, Yanling Xiao and Jannie Borst

Division of Immunology, Netherlands Cancer Institute, 1066 CX Amsterdam, Netherlands

Address correspondence to Jannie Borst, Division of Immunology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, Netherlands. Phone: 31-20-5122056; Fax: 31-20-5122057; email: j.borst{at}nki.nl

CD27, like CD28, acts in concert with the T cell receptor to support T cell expansion. Using CD27-/- mice, we have shown earlier that CD27 determines the magnitude of primary and memory T cell responses to influenza virus. Here, we have examined the relative contributions of CD27 and CD28 to generation of the virus-specific effector T cell pool and its establishment at the site of infection (the lung), using CD27-/-, CD28-/-, and CD27/CD28-/- mice. We find that primary and memory CD8+ T cell responses to influenza virus are dependent on the collective contribution of both receptors. In the primary response, CD27 and CD28 impact to a similar extent on expansion of virus-specific T cells in draining lymph nodes. CD27 is the principle determinant for accumulation of virus-specific T cells in the lung because it can sustain this response in CD28-/- mice. Unlike CD28, CD27 does not affect cell cycle activity, but promotes survival of activated T cells throughout successive rounds of division at the site of priming and may do so at the site of infection as well. CD27 was found to rescue CD28-/- T cells from death at the onset of division, explaining its capacity to support a T cell response in absence of CD28.

Key Words: costimulation • apoptosis • influenza virus • MHC tetramer • CFSE


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