The Journal of Experimental Medicine
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Published online 30 June 2003 doi:10.1084/jem.20022230
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© Rockefeller University Press, 0022-1007/2003/7/123 $5.00
The Journal of Experimental Medicine, Volume 198, Number 1, 123-132

Restricted Clonal Expression of IL-2 By Naive T Cells Reflects Differential Dynamic Interactions with Dendritic Cells

Vincent Hurez1, Arman Saparov1, Albert Tousson2, Michael J. Fuller2, Takekazu Kubo1,4, James Oliver1, Benjamin T. Weaver1 and Casey T. Weaver1,3

1 Department of Pathology, University of Alabama at Birmingham, Birmingham, AL 35294
2 Department of Cell Biology, University of Alabama at Birmingham, Birmingham, AL 35294
3 Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294
4 Biological Research Laboratories, Sankyo Co., Ltd., Tokyo 140-8710, Japan

Address correspondence to Casey T. Weaver, 870 Bevill Biomedical Research Building, 845 19th St. South, Birmingham, AL 35294-2170. Phone: 205-975-5537; Fax: 205-975-8310; E-mail: weaver{at}path.uab.edu

Limited frequencies of T cells express IL-2 in primary antigenic responses, despite activation marker expression and proliferation by most clonal members. To define the basis for restricted IL-2 expression, a videomicroscopic system and IL-2 reporter transgenic model were used to characterize dendritic cell (DC)–T cell interactions. T cells destined to produce IL-2 required prolonged interactions with DCs, whereas most T cells established only transient interactions with DCs and were activated, but did not express IL-2. Extended conjugation of T cells with DCs was not always sufficient to initiate IL-2 expression. Thus, there is intrinsic variability in clonal T cell populations that restricts IL-2 commitment, and prolonged engagement with mature DCs is necessary, but not sufficient, for IL-2 gene transcription.

Key Words: transgenic mice • T lymphocytes • lymphocyte activation • interleukin 2 • dendritic cells


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