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Brief Definitive Report |
Address correspondence to Dr. Stefan H.I. Kappe, Michael Heidelberger Division, Dept. of Pathology, New York University School of Medicine, 550 First Ave., MSB127, New York, NY 10016. Phone: 212-263-7870; Fax: 212-263-8179; E-mail: kappes01{at}endeavor.med.nyu.edu
Malaria parasite species that infect mammals, including humans, must first take up residence in hepatic host cells as exoerythrocytic forms (EEF) before initiating infection of red blood cells that leads to malaria disease. Despite the importance of hepatic stages for immunity against malaria, little is known about their biology and antigenic composition. Here, we show that sporozoites, the parasites' transmission stage that resides in the mosquito vector salivary glands, can transform into early EEF without intracellular residence in host hepatocytes. The morphological sequence of transformation and the expression of proteins in the EEF appear indistinguishable from parasites that develop within host cells. Transformation depends on temperature elevation to 37°C and serum. Our findings demonstrate that residence in a host hepatocyte or specific host cell–derived factors are not necessary to bring about the profound morphological and biochemical changes of the parasite that occur after its transmission from vector to mammalian host.
Key Words: Plasmodium • liver stage • hepatocyte • temperature • axenic culture
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