Published 3 March 2003. doi:10.1084/jem.20021756
© Rockefeller University Press,
0022-1007/2003/3/633 $5.00
The Journal of Experimental Medicine, Volume 197, Number 5, 633-642
Infectious Hepatitis C Virus Pseudo-particles Containing Functional E1E2 Envelope Protein Complexes
Birke Bartosch1,
Jean Dubuisson2 and
François-Loïc Cosset1
1 Laboratoire de Vectorologie Rétrovirale et Thérapie Génique, Institut National de la Santé et de la Recherche Médicale U412, IFR 128, Ecole Normale Supérieure de Lyon, 69364 Lyon Cedex 07, France
2 Centre National de la Recherche Scientifique-UPR2511, Institut de Biologie de Lille, Institut Pasteur de Lille, 59021 Lille Cedex, France
Address correspondence to François-Loïc Cosset, LVRTG, ENS de Lyon, 46 Allée d'Italie, 69364 Lyon Cedex 07, France. Phone: 33-472-72-87-32; Fax: 33-472-72-80-80; E-mail: flcosset{at}ens-lyon.fr
The study of hepatitis C virus (HCV), a major cause of chronic liver disease, has been hampered by the lack of a cell culture system supporting its replication. Here, we have successfully generated infectious pseudo-particles that were assembled by displaying unmodified and functional HCV glycoproteins onto retroviral and lentiviral core particles. The presence of a green fluorescent protein marker gene packaged within these HCV pseudo-particles allowed reliable and fast determination of infectivity mediated by the HCV glycoproteins. Primary hepatocytes as well as hepato-carcinoma cells were found to be the major targets of infection in vitro. High infectivity of the pseudo-particles required both E1 and E2 HCV glycoproteins, and was neutralized by sera from HCV-infected patients and by some anti-E2 monoclonal antibodies. In addition, these pseudo-particles allowed investigation of the role of putative HCV receptors. Although our results tend to confirm their involvement, they provide evidence that neither LDLr nor CD81 is sufficient to mediate HCV cell entry. Altogether, these studies indicate that these pseudo-particles may mimic the early infection steps of parental HCV and will be suitable for the development of much needed new antiviral therapies.
Key Words: hepatitis viral assembly glycoproteins receptor neutralization

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-
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(2008). Broadly neutralizing human monoclonal antibodies to the hepatitis C virus E2 glycoprotein. J. Gen. Virol.
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-
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[Full Text]
-
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[Full Text]
-
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[Full Text]
-
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-
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(2008). Identification of a Broadly Cross-Reacting and Neutralizing Human Monoclonal Antibody Directed against the Hepatitis C Virus E2 Protein. J. Virol.
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[Abstract]
[Full Text]
-
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[Full Text]
-
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(2008). Effect of Cell Polarization on Hepatitis C Virus Entry. J. Virol.
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[Abstract]
[Full Text]
-
Zheng, A., Yuan, F., Li, Y., Zhu, F., Hou, P., Li, J., Song, X., Ding, M., Deng, H.
(2007). Claudin-6 and Claudin-9 Function as Additional Coreceptors for Hepatitis C Virus. J. Virol.
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[Abstract]
[Full Text]
-
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[Abstract]
[Full Text]
-
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(2007). Determination of the human antibody response to the epitope defined by the hepatitis C virus-neutralizing monoclonal antibody AP33. J. Gen. Virol.
88: 2991-3001
[Abstract]
[Full Text]
-
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(2007). Assembly of Hepatitis B Virus Envelope Proteins onto a Lentivirus Pseudotype That Infects Primary Human Hepatocytes. J. Virol.
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[Abstract]
[Full Text]
-
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(2007). Human combinatorial libraries yield rare antibodies that broadly neutralize hepatitis C virus. Proc. Natl. Acad. Sci. USA
104: 16269-16274
[Abstract]
[Full Text]
-
Delgrange, D., Pillez, A., Castelain, S., Cocquerel, L., Rouille, Y., Dubuisson, J., Wakita, T., Duverlie, G., Wychowski, C.
(2007). Robust production of infectious viral particles in Huh-7 cells by introducing mutations in hepatitis C virus structural proteins. J. Gen. Virol.
88: 2495-2503
[Abstract]
[Full Text]
-
Tellinghuisen, T. L., Evans, M. J., von Hahn, T., You, S., Rice, C. M.
(2007). Studying Hepatitis C Virus: Making the Best of a Bad Virus. J. Virol.
81: 8853-8867
[Full Text]
-
Lavillette, D., Pecheur, E.-I., Donot, P., Fresquet, J., Molle, J., Corbau, R., Dreux, M., Penin, F., Cosset, F.-L.
(2007). Characterization of Fusion Determinants Points to the Involvement of Three Discrete Regions of Both E1 and E2 Glycoproteins in the Membrane Fusion Process of Hepatitis C Virus. J. Virol.
81: 8752-8765
[Abstract]
[Full Text]
-
Tani, H., Komoda, Y., Matsuo, E., Suzuki, K., Hamamoto, I., Yamashita, T., Moriishi, K., Fujiyama, K., Kanto, T., Hayashi, N., Owsianka, A., Patel, A. H., Whitt, M. A., Matsuura, Y.
(2007). Replication-Competent Recombinant Vesicular Stomatitis Virus Encoding Hepatitis C Virus Envelope Proteins. J. Virol.
81: 8601-8612
[Abstract]
[Full Text]
-
Falkowska, E., Kajumo, F., Garcia, E., Reinus, J., Dragic, T.
(2007). Hepatitis C Virus Envelope Glycoprotein E2 Glycans Modulate Entry, CD81 Binding, and Neutralization. J. Virol.
81: 8072-8079
[Abstract]
[Full Text]
-
Helle, F., Goffard, A., Morel, V., Duverlie, G., McKeating, J., Keck, Z.-Y., Foung, S., Penin, F., Dubuisson, J., Voisset, C.
(2007). The Neutralizing Activity of Anti-Hepatitis C Virus Antibodies Is Modulated by Specific Glycans on the E2 Envelope Protein. J. Virol.
81: 8101-8111
[Abstract]
[Full Text]
-
Randall, G., Panis, M., Cooper, J. D., Tellinghuisen, T. L., Sukhodolets, K. E., Pfeffer, S., Landthaler, M., Landgraf, P., Kan, S., Lindenbach, B. D., Chien, M., Weir, D. B., Russo, J. J., Ju, J., Brownstein, M. J., Sheridan, R., Sander, C., Zavolan, M., Tuschl, T., Rice, C. M.
(2007). Cellular cofactors affecting hepatitis C virus infection and replication. Proc. Natl. Acad. Sci. USA
104: 12884-12889
[Abstract]
[Full Text]
-
Petit, J.-M., Minello, A., Duvillard, L., Jooste, V., Monier, S., Texier, V., Bour, J.-B., Poussier, A., Gambert, P., Verges, B., Hillon, P.
(2007). Cell surface expression of LDL receptor in chronic hepatitis C: correlation with viral load. Am. J. Physiol. Endocrinol. Metab.
293: E416-E420
[Abstract]
[Full Text]
-
Akazawa, D., Date, T., Morikawa, K., Murayama, A., Miyamoto, M., Kaga, M., Barth, H., Baumert, T. F., Dubuisson, J., Wakita, T.
(2007). CD81 Expression Is Important for the Permissiveness of Huh7 Cell Clones for Heterogeneous Hepatitis C Virus Infection. J. Virol.
81: 5036-5045
[Abstract]
[Full Text]
-
Zhang, P., Wu, C. G., Mihalik, K., Virata-Theimer, M. L., Yu, M.-y. W., Alter, H. J., Feinstone, S. M.
(2007). Hepatitis C virus epitope-specific neutralizing antibodies in Igs prepared from human plasma. Proc. Natl. Acad. Sci. USA
104: 8449-8454
[Abstract]
[Full Text]
-
Schaller, T., Appel, N., Koutsoudakis, G., Kallis, S., Lohmann, V., Pietschmann, T., Bartenschlager, R.
(2007). Analysis of Hepatitis C Virus Superinfection Exclusion by Using Novel Fluorochrome Gene-Tagged Viral Genomes. J. Virol.
81: 4591-4603
[Abstract]
[Full Text]
-
Basu, A., Kanda, T., Beyene, A., Saito, K., Meyer, K., Ray, R.
(2007). Sulfated Homologues of Heparin Inhibit Hepatitis C Virus Entry into Mammalian Cells. J. Virol.
81: 3933-3941
[Abstract]
[Full Text]
-
Tscherne, D. M., Evans, M. J., von Hahn, T., Jones, C. T., Stamataki, Z., McKeating, J. A., Lindenbach, B. D., Rice, C. M.
(2007). Superinfection Exclusion in Cells Infected with Hepatitis C Virus. J. Virol.
81: 3693-3703
[Abstract]
[Full Text]
-
Pestka, J. M., Zeisel, M. B., Blaser, E., Schurmann, P., Bartosch, B., Cosset, F.-L., Patel, A. H., Meisel, H., Baumert, J., Viazov, S., Rispeter, K., Blum, H. E., Roggendorf, M., Baumert, T. F.
(2007). Rapid induction of virus-neutralizing antibodies and viral clearance in a single-source outbreak of hepatitis C. Proc. Natl. Acad. Sci. USA
104: 6025-6030
[Abstract]
[Full Text]
-
Chapel, C., Garcia, C., Bartosch, B., Roingeard, P., Zitzmann, N., Cosset, F.-L., Dubuisson, J., Dwek, R. A., Trepo, C., Zoulim, F., Durantel, D.
(2007). Reduction of the infectivity of hepatitis C virus pseudoparticles by incorporation of misfolded glycoproteins induced by glucosidase inhibitors. J. Gen. Virol.
88: 1133-1143
[Abstract]
[Full Text]
-
Drummer, H. E., Boo, I., Poumbourios, P.
(2007). Mutagenesis of a conserved fusion peptide-like motif and membrane-proximal heptad-repeat region of hepatitis C virus glycoprotein E1. J. Gen. Virol.
88: 1144-1148
[Abstract]
[Full Text]
-
Grove, J., Huby, T., Stamataki, Z., Vanwolleghem, T., Meuleman, P., Farquhar, M., Schwarz, A., Moreau, M., Owen, J. S., Leroux-Roels, G., Balfe, P., McKeating, J. A.
(2007). Scavenger Receptor BI and BII Expression Levels Modulate Hepatitis C Virus Infectivity. J. Virol.
81: 3162-3169
[Abstract]
[Full Text]
-
Ciczora, Y., Callens, N., Penin, F., Pecheur, E.-I., Dubuisson, J.
(2007). Transmembrane Domains of Hepatitis C Virus Envelope Glycoproteins: Residues Involved in E1E2 Heterodimerization and Involvement of These Domains in Virus Entry. J. Virol.
81: 2372-2381
[Abstract]
[Full Text]
-
Keck, Z.-Y., Xia, J., Cai, Z., Li, T.-K., Owsianka, A. M., Patel, A. H., Luo, G., Foung, S. K. H.
(2007). Immunogenic and Functional Organization of Hepatitis C Virus (HCV) Glycoprotein E2 on Infectious HCV Virions. J. Virol.
81: 1043-1047
[Abstract]
[Full Text]
-
Kapadia, S. B., Barth, H., Baumert, T., McKeating, J. A., Chisari, F. V.
(2007). Initiation of Hepatitis C Virus Infection Is Dependent on Cholesterol and Cooperativity between CD81 and Scavenger Receptor B Type I. J. Virol.
81: 374-383
[Abstract]
[Full Text]
-
Meertens, L., Bertaux, C., Dragic, T.
(2006). Hepatitis C Virus Entry Requires a Critical Postinternalization Step and Delivery to Early Endosomes via Clathrin-Coated Vesicles. J. Virol.
80: 11571-11578
[Abstract]
[Full Text]
-
Flint, M., von Hahn, T., Zhang, J., Farquhar, M., Jones, C. T., Balfe, P., Rice, C. M., McKeating, J. A.
(2006). Diverse CD81 Proteins Support Hepatitis C Virus Infection. J. Virol.
80: 11331-11342
[Abstract]
[Full Text]
-
Nakai, K., Okamoto, T., Kimura-Someya, T., Ishii, K., Lim, C. K., Tani, H., Matsuo, E., Abe, T., Mori, Y., Suzuki, T., Miyamura, T., Nunberg, J. H., Moriishi, K., Matsuura, Y.
(2006). Oligomerization of Hepatitis C Virus Core Protein Is Crucial for Interaction with the Cytoplasmic Domain of E1 Envelope Protein. J. Virol.
80: 11265-11273
[Abstract]
[Full Text]
-
Barth, H., Schnober, E. K., Zhang, F., Linhardt, R. J., Depla, E., Boson, B., Cosset, F.-L., Patel, A. H., Blum, H. E., Baumert, T. F.
(2006). Viral and Cellular Determinants of the Hepatitis C Virus Envelope-Heparan Sulfate Interaction. J. Virol.
80: 10579-10590
[Abstract]
[Full Text]
-
Claus, C., Hofmann, J., Uberla, K., Liebert, U. G.
(2006). Rubella virus pseudotypes and a cell-cell fusion assay as tools for functional analysis of the rubella virus E2 and E1 envelope glycoproteins.. J. Gen. Virol.
87: 3029-3037
[Abstract]
[Full Text]
-
Voisset, C., de Beeck, A. O., Horellou, P., Dreux, M., Gustot, T., Duverlie, G., Cosset, F.-L., Vu-Dac, N., Dubuisson, J.
(2006). High-density lipoproteins reduce the neutralizing effect of hepatitis C virus (HCV)-infected patient antibodies by promoting HCV entry. J. Gen. Virol.
87: 2577-2581
[Abstract]
[Full Text]
-
Codran, A., Royer, C., Jaeck, D., Bastien-Valle, M., Baumert, T. F., Kieny, M. P., Pereira, C. A., Martin, J.-P.
(2006). Entry of hepatitis C virus pseudotypes into primary human hepatocytes by clathrin-dependent endocytosis. J. Gen. Virol.
87: 2583-2593
[Abstract]
[Full Text]
-
Owsianka, A. M., Timms, J. M., Tarr, A. W., Brown, R. J. P., Hickling, T. P., Szwejk, A., Bienkowska-Szewczyk, K., Thomson, B. J., Patel, A. H., Ball, J. K.
(2006). Identification of conserved residues in the e2 envelope glycoprotein of the hepatitis C virus that are critical for CD81 binding.. J. Virol.
80: 8695-8704
[Abstract]
[Full Text]
-
Helle, F., Wychowski, C., Vu-Dac, N., Gustafson, K. R., Voisset, C., Dubuisson, J.
(2006). Cyanovirin-N Inhibits Hepatitis C Virus Entry by Binding to Envelope Protein Glycans. J. Biol. Chem.
281: 25177-25183
[Abstract]
[Full Text]
-
Drummer, H. E., Boo, I., Maerz, A. L., Poumbourios, P.
(2006). A conserved gly436-trp-leu-ala-gly-leu-phe-tyr motif in hepatitis C virus glycoprotein e2 is a determinant of CD81 binding and viral entry.. J. Virol.
80: 7844-7853
[Abstract]
[Full Text]
-
Kronenberger, B., Herrmann, E., Hofmann, W. P., Wedemeyer, H., Sester, M., Mihm, U., Ghaliai, T., Zeuzem, S., Sarrazin, C.
(2006). Dynamics of CD81 expression on lymphocyte subsets during interferon-{alpha}-based antiviral treatment of patients with chronic hepatitis C. J. Leukoc. Biol.
80: 298-308
[Abstract]
[Full Text]
-
Blanchard, E., Belouzard, S., Goueslain, L., Wakita, T., Dubuisson, J., Wychowski, C., Rouille, Y.
(2006). Hepatitis C Virus Entry Depends on Clathrin-Mediated Endocytosis. J. Virol.
80: 6964-6972
[Abstract]
[Full Text]
-
Dreux, M., Pietschmann, T., Granier, C., Voisset, C., Ricard-Blum, S., Mangeot, P.-E., Keck, Z., Foung, S., Vu-Dac, N., Dubuisson, J., Bartenschlager, R., Lavillette, D., Cosset, F.-L.
(2006). High Density Lipoprotein Inhibits Hepatitis C Virus-neutralizing Antibodies by Stimulating Cell Entry via Activation of the Scavenger Receptor BI. J. Biol. Chem.
281: 18285-18295
[Abstract]
[Full Text]
-
Koutsoudakis, G., Kaul, A., Steinmann, E., Kallis, S., Lohmann, V., Pietschmann, T., Bartenschlager, R.
(2006). Characterization of the early steps of hepatitis C virus infection by using luciferase reporter viruses.. J. Virol.
80: 5308-5320
[Abstract]
[Full Text]
-
Cocquerel, L., Voisset, C., Dubuisson, J.
(2006). Hepatitis C virus entry: potential receptors and their biological functions.. J. Gen. Virol.
87: 1075-1084
[Abstract]
[Full Text]
-
Bertaux, C., Dragic, T.
(2006). Different domains of CD81 mediate distinct stages of hepatitis C virus pseudoparticle entry.. J. Virol.
80: 4940-4948
[Abstract]
[Full Text]
-
Appel, N., Schaller, T., Penin, F., Bartenschlager, R.
(2006). From Structure to Function: New Insights into Hepatitis C Virus RNA Replication. J. Biol. Chem.
281: 9833-9836
[Full Text]
-
Lavillette, D., Barbouche, R., Yao, Y., Boson, B., Cosset, F.-L., Jones, I. M., Fenouillet, E.
(2006). Significant Redox Insensitivity of the Functions of the SARS-CoV Spike Glycoprotein: COMPARISON WITH HIV ENVELOPE. J. Biol. Chem.
281: 9200-9204
[Abstract]
[Full Text]
-
Eren, R., Landstein, D., Terkieltaub, D., Nussbaum, O., Zauberman, A., Ben-Porath, J., Gopher, J., Buchnick, R., Kovjazin, R., Rosenthal-Galili, Z., Aviel, S., Ilan, E., Shoshany, Y., Neville, L., Waisman, T., Ben-Moshe, O., Kischitsky, A., Foung, S. K. H., Keck, Z.-Y., Pappo, O., Eid, A., Jurim, O., Zamir, G., Galun, E., Dagan, S.
(2006). Preclinical Evaluation of Two Neutralizing Human Monoclonal Antibodies against Hepatitis C Virus (HCV): a Potential Treatment To Prevent HCV Reinfection in Liver Transplant Patients. J. Virol.
80: 2654-2664
[Abstract]
[Full Text]
-
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(2006). Subcellular Localization of Hepatitis C Virus Structural Proteins in a Cell Culture System That Efficiently Replicates the Virus. J. Virol.
80: 2832-2841
[Abstract]
[Full Text]
-
Lavillette, D., Bartosch, B., Nourrisson, D., Verney, G., Cosset, F.-L., Penin, F., Pecheur, E.-I.
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281: 3909-3917
[Abstract]
[Full Text]
-
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[Full Text]
-
Kobayashi, M., Bennett, M. C., Bercot, T., Singh, I. R.
(2006). Functional Analysis of Hepatitis C Virus Envelope Proteins, Using a Cell-Cell Fusion Assay. J. Virol.
80: 1817-1825
[Abstract]
[Full Text]
-
Broer, R., Boson, B., Spaan, W., Cosset, F.-L., Corver, J.
(2006). Important Role for the Transmembrane Domain of Severe Acute Respiratory Syndrome Coronavirus Spike Protein during Entry. J. Virol.
80: 1302-1310
[Abstract]
[Full Text]
-
Sandrin, V., Cosset, F.-L.
(2006). Intracellular Versus Cell Surface Assembly of Retroviral Pseudotypes Is Determined by the Cellular Localization of the Viral Glycoprotein, Its Capacity to Interact with Gag, and the Expression of the Nef Protein. J. Biol. Chem.
281: 528-542
[Abstract]
[Full Text]
-
Callens, N., Ciczora, Y., Bartosch, B., Vu-Dac, N., Cosset, F.-L., Pawlotsky, J.-M., Penin, F., Dubuisson, J.
(2005). Basic Residues in Hypervariable Region 1 of Hepatitis C Virus Envelope Glycoprotein E2 Contribute to Virus Entry. J. Virol.
79: 15331-15341
[Abstract]
[Full Text]
-
Sandrin, V., Boulanger, P., Penin, F., Granier, C., Cosset, F.-L., Bartosch, B.
(2005). Assembly of functional hepatitis C virus glycoproteins on infectious pseudoparticles occurs intracellularly and requires concomitant incorporation of E1 and E2 glycoproteins. J. Gen. Virol.
86: 3189-3199
[Abstract]
[Full Text]
-
Cai, Z., Zhang, C., Chang, K.-S., Jiang, J., Ahn, B.-C., Wakita, T., Liang, T. J., Luo, G.
(2005). Robust Production of Infectious Hepatitis C Virus (HCV) from Stably HCV cDNA-Transfected Human Hepatoma Cells. J. Virol.
79: 13963-13973
[Abstract]
[Full Text]
-
Keck, Z.-Y., Li, T.-K., Xia, J., Bartosch, B., Cosset, F.-L., Dubuisson, J., Foung, S. K. H.
(2005). Analysis of a Highly Flexible Conformational Immunogenic Domain A in Hepatitis C Virus E2. J. Virol.
79: 13199-13208
[Abstract]
[Full Text]
-
Ciczora, Y., Callens, N., Montpellier, C., Bartosch, B., Cosset, F.-L., De Beeck, A. O., Dubuisson, J.
(2005). Contribution of the charged residues of hepatitis C virus glycoprotein E2 transmembrane domain to the functions of the E1E2 heterodimer. J. Gen. Virol.
86: 2793-2798
[Abstract]
[Full Text]
-
Yamada, E., Montoya, M., Schuettler, C. G., Hickling, T. P., Tarr, A. W., Vitelli, A., Dubuisson, J., Patel, A. H., Ball, J. K., Borrow, P.
(2005). Analysis of the binding of hepatitis C virus genotype 1a and 1b E2 glycoproteins to peripheral blood mononuclear cell subsets. J. Gen. Virol.
86: 2507-2512
[Abstract]
[Full Text]
-
Owsianka, A., Tarr, A. W., Juttla, V. S., Lavillette, D., Bartosch, B., Cosset, F.-L., Ball, J. K., Patel, A. H.
(2005). Monoclonal Antibody AP33 Defines a Broadly Neutralizing Epitope on the Hepatitis C Virus E2 Envelope Glycoprotein. J. Virol.
79: 11095-11104
[Abstract]
[Full Text]
-
Zhang, C., Cai, Z., Kim, Y.-C., Kumar, R., Yuan, F., Shi, P.-Y., Kao, C., Luo, G.
(2005). Stimulation of Hepatitis C Virus (HCV) Nonstructural Protein 3 (NS3) Helicase Activity by the NS3 Protease Domain and by HCV RNA-Dependent RNA Polymerase. J. Virol.
79: 8687-8697
[Abstract]
[Full Text]
-
Bartenschlager, R., Pietschmann, T.
(2005). Efficient hepatitis C virus cell culture system: What a difference the host cell makes. Proc. Natl. Acad. Sci. USA
102: 9739-9740
[Full Text]
-
Bartosch, B., Verney, G., Dreux, M., Donot, P., Morice, Y., Penin, F., Pawlotsky, J.-M., Lavillette, D., Cosset, F.-L.
(2005). An Interplay between Hypervariable Region 1 of the Hepatitis C Virus E2 Glycoprotein, the Scavenger Receptor BI, and High-Density Lipoprotein Promotes both Enhancement of Infection and Protection against Neutralizing Antibodies. J. Virol.
79: 8217-8229
[Abstract]
[Full Text]
-
Goffard, A., Callens, N., Bartosch, B., Wychowski, C., Cosset, F.-L., Montpellier, C., Dubuisson, J.
(2005). Role of N-Linked Glycans in the Functions of Hepatitis C Virus Envelope Glycoproteins. J. Virol.
79: 8400-8409
[Abstract]
[Full Text]
-
Zhong, J., Gastaminza, P., Cheng, G., Kapadia, S., Kato, T., Burton, D. R., Wieland, S. F., Uprichard, S. L., Wakita, T., Chisari, F. V.
(2005). Robust hepatitis C virus infection in vitro. Proc. Natl. Acad. Sci. USA
102: 9294-9299
[Abstract]
[Full Text]
-
Lavillette, D., Morice, Y., Germanidis, G., Donot, P., Soulier, A., Pagkalos, E., Sakellariou, G., Intrator, L., Bartosch, B., Pawlotsky, J.-M., Cosset, F.-L.
(2005). Human Serum Facilitates Hepatitis C Virus Infection, and Neutralizing Responses Inversely Correlate with Viral Replication Kinetics at the Acute Phase of Hepatitis C Virus Infection. J. Virol.
79: 6023-6034
[Abstract]
[Full Text]
-
Barth, H., Cerino, R., Arcuri, M., Hoffmann, M., Schurmann, P., Adah, M. I., Gissler, B., Zhao, X., Ghisetti, V., Lavezzo, B., Blum, H. E., von Weizsacker, F., Vitelli, A., Scarselli, E., Baumert, T. F.
(2005). Scavenger Receptor Class B Type I and Hepatitis C Virus Infection of Primary Tupaia Hepatocytes. J. Virol.
79: 5774-5785
[Abstract]
[Full Text]
-
Meunier, J.-C., Engle, R. E., Faulk, K., Zhao, M., Bartosch, B., Alter, H., Emerson, S. U., Cosset, F.-L., Purcell, R. H., Bukh, J.
(2005). Evidence for cross-genotype neutralization of hepatitis C virus pseudo-particles and enhancement of infectivity by apolipoprotein C1. Proc. Natl. Acad. Sci. USA
102: 4560-4565
[Abstract]
[Full Text]
-
Voisset, C., Callens, N., Blanchard, E., Op De Beeck, A., Dubuisson, J., Vu-Dac, N.
(2005). High Density Lipoproteins Facilitate Hepatitis C Virus Entry through the Scavenger Receptor Class B Type I. J. Biol. Chem.
280: 7793-7799
[Abstract]
[Full Text]
-
Meyer, K., Beyene, A., Bowlin, T. L., Basu, A., Ray, R.
(2004). Coexpression of Hepatitis C Virus E1 and E2 Chimeric Envelope Glycoproteins Displays Separable Ligand Sensitivity and Increases Pseudotype Infectious Titer. J. Virol.
78: 12838-12847
[Abstract]
[Full Text]
-
Yi, L., Li, Z., Yuan, K., Qu, X., Chen, J., Wang, G., Zhang, H., Luo, H., Zhu, L., Jiang, P., Chen, L., Shen, Y., Luo, M., Zuo, G., Hu, J., Duan, D., Nie, Y., Shi, X., Wang, W., Han, Y., Li, T., Liu, Y., Ding, M., Deng, H., Xu, X.
(2004). Small Molecules Blocking the Entry of Severe Acute Respiratory Syndrome Coronavirus into Host Cells. J. Virol.
78: 11334-11339
[Abstract]
[Full Text]
-
Cormier, E. G., Durso, R. J., Tsamis, F., Boussemart, L., Manix, C., Olson, W. C., Gardner, J. P., Dragic, T.
(2004). L-SIGN (CD209L) and DC-SIGN (CD209) mediate transinfection of liver cells by hepatitis C virus. Proc. Natl. Acad. Sci. USA
101: 14067-14072
[Abstract]
[Full Text]
-
Fernandez, J., Taylor, D., Morhardt, D. R., Mihalik, K., Puig, M., Rice, C. M., Feinstone, S. M., Major, M. E.
(2004). Long-Term Persistence of Infection in Chimpanzees Inoculated with an Infectious Hepatitis C Virus Clone Is Associated with a Decrease in the Viral Amino Acid Substitution Rate and Low Levels of Heterogeneity. J. Virol.
78: 9782-9789
[Abstract]
[Full Text]
-
Giroglou, T., Cinatl, J. Jr., Rabenau, H., Drosten, C., Schwalbe, H., Doerr, H. W., von Laer, D.
(2004). Retroviral Vectors Pseudotyped with Severe Acute Respiratory Syndrome Coronavirus S Protein. J. Virol.
78: 9007-9015
[Abstract]
[Full Text]
-
Steinmann, D., Barth, H., Gissler, B., Schurmann, P., Adah, M. I., Gerlach, J. T., Pape, G. R., Depla, E., Jacobs, D., Maertens, G., Patel, A. H., Inchauspe, G., Liang, T. J., Blum, H. E., Baumert, T. F.
(2004). Inhibition of Hepatitis C Virus-Like Particle Binding to Target Cells by Antiviral Antibodies in Acute and Chronic Hepatitis C. J. Virol.
78: 9030-9040
[Abstract]
[Full Text]
-
Keck, Z.-Y., Op De Beeck, A., Hadlock, K. G., Xia, J., Li, T.-K., Dubuisson, J., Foung, S. K. H.
(2004). Hepatitis C Virus E2 Has Three Immunogenic Domains Containing Conformational Epitopes with Distinct Properties and Biological Functions. J. Virol.
78: 9224-9232
[Abstract]
[Full Text]
-
McKeating, J. A., Zhang, L. Q., Logvinoff, C., Flint, M., Zhang, J., Yu, J., Butera, D., Ho, D. D., Dustin, L. B., Rice, C. M., Balfe, P.
(2004). Diverse Hepatitis C Virus Glycoproteins Mediate Viral Infection in a CD81-Dependent Manner. J. Virol.
78: 8496-8505
[Abstract]
[Full Text]
-
Lozach, P.-Y., Amara, A., Bartosch, B., Virelizier, J.-L., Arenzana-Seisdedos, F., Cosset, F.-L., Altmeyer, R.
(2004). C-type Lectins L-SIGN and DC-SIGN Capture and Transmit Infectious Hepatitis C Virus Pseudotype Particles. J. Biol. Chem.
279: 32035-32045
[Abstract]
[Full Text]
-
Drummer, H. E., Poumbourios, P.
(2004). Hepatitis C Virus Glycoprotein E2 Contains a Membrane-proximal Heptad Repeat Sequence That Is Essential for E1E2 Glycoprotein Heterodimerization and Viral Entry. J. Biol. Chem.
279: 30066-30072
[Abstract]
[Full Text]
-
Logvinoff, C., Major, M. E., Oldach, D., Heyward, S., Talal, A., Balfe, P., Feinstone, S. M., Alter, H., Rice, C. M., McKeating, J. A.
(2004). Neutralizing antibody response during acute and chronic hepatitis C virus infection. Proc. Natl. Acad. Sci. USA
101: 10149-10154
[Abstract]
[Full Text]