Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents

This Article Full Text Full Text (PDF, 342K) PPT slides of all figures Alert me when this article is cited Citation Map Services Email this article Similar articles in this journal Similar articles in PubMed Alert me to new content in the JEM Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Fillatreau, S. Articles by Gray, D. Search for Related Content PubMed PubMed Citation Articles by Fillatreau, S. Articles by Gray, D. Social Bookmarking                      What's this?

T Cell Accumulation in B Cell Follicles Is Regulated by Dendritic Cells and Is Independent of B Cell Activation

Simon Fillatreau and David Gray

Institute of Cell, Animal and Population Biology, University of Edinburgh, Edinburgh EH9 3JT, United Kingdom

Address correspondence to David Gray, Institute of Cell, Animal and Population Biology, University of Edinburgh, Ashworth Laboratories, King's Buildings, West Mains Road, Edinburgh EH9 3JT, United Kingdom. Phone: 44-131-650-5500; Fax: 44-131-650-7322; E-mail: d.gray{at}ed.ac.uk

We investigated the mechanism of CD4 T cell accumulation in B cell follicles after immunization. Follicular T cell numbers were correlated with the number of B cells, indicating B cell control of the niche that T cells occupy. Despite this, we found no role for B cells in the follicular migration of T cells. Instead, T cells are induced to migrate into B cell follicles entirely as a result of interaction with dendritic cells (DCs). Migration relies on CD40-dependent maturation of DCs, as it did not occur in CD40-deficient mice but was reconstituted with CD40⁺ DCs. Restoration was not achieved by the activation of DCs with bacterial activators (e.g., lipopolysaccharide, CpG), but was by the injection of OX40L–huIgG1 fusion protein. Crucially, the up-regulation of OX40L (on antigen-presenting cells) and CXCR-5 (on T cells) are CD40-dependent events and we show that T cells do not migrate to follicles in immunized OX40-deficient mice.

Key Words: T lymphocyte migration • B lymphocytes • OX40 • CXCR5 • lymphoid follicles

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

• Barr, T. A., Brown, S., Mastroeni, P., Gray, D. (2009). B Cell Intrinsic MyD88 Signals Drive IFN-{gamma} Production from T Cells and Control Switching to IgG2c. J. Immunol. 183: 1005-1012 [Abstract] [Full Text]  
• Tsuji, M., Komatsu, N., Kawamoto, S., Suzuki, K., Kanagawa, O., Honjo, T., Hori, S., Fagarasan, S. (2009). Preferential Generation of Follicular B Helper T Cells from Foxp3+ T Cells in Gut Peyer's Patches. Science 323: 1488-1492 [Abstract] [Full Text]  
• Tonti, E., Galli, G., Malzone, C., Abrignani, S., Casorati, G., Dellabona, P. (2009). NKT-cell help to B lymphocytes can occur independently of cognate interaction. Blood 113: 370-376 [Abstract] [Full Text]  
• Jenkins, S. J., Perona-Wright, G., MacDonald, A. S. (2008). Full Development of Th2 Immunity Requires Both Innate and Adaptive Sources of CD154. J. Immunol. 180: 8083-8092 [Abstract] [Full Text]  
• Kalies, K., Konig, P., Zhang, Y.-M., Deierling, M., Barthelmann, J., Stamm, C., Westermann, J. (2008). Nonoverlapping Expression of IL10, IL12p40, and IFN{gamma} mRNA in the Marginal Zone and T Cell Zone of the Spleen after Antigenic Stimulation. J. Immunol. 180: 5457-5465 [Abstract] [Full Text]  
• Lampropoulou, V., Hoehlig, K., Roch, T., Neves, P., Gomez, E. C., Sweenie, C. H., Hao, Y., Freitas, A. A., Steinhoff, U., Anderton, S. M., Fillatreau, S. (2008). TLR-Activated B Cells Suppress T Cell-Mediated Autoimmunity. J. Immunol. 180: 4763-4773 [Abstract] [Full Text]  
• MacLeod, M., Kwakkenbos, M. J., Crawford, A., Brown, S., Stockinger, B., Schepers, K., Schumacher, T., Gray, D. (2006). CD4 memory T cells survive and proliferate but fail to differentiate in the absence of CD40. JEM 203: 897-906 [Abstract] [Full Text]  
• Crawford, A., MacLeod, M., Schumacher, T., Corlett, L., Gray, D. (2006). Primary T Cell Expansion and Differentiation In Vivo Requires Antigen Presentation by B Cells. J. Immunol. 176: 3498-3506 [Abstract] [Full Text]  
• Johansson-Lindbom, B., Svensson, M., Pabst, O., Palmqvist, C., Marquez, G., Forster, R., Agace, W. W. (2005). Functional specialization of gut CD103+ dendritic cells in the regulation of tissue-selective T cell homing. JEM 202: 1063-1073 [Abstract] [Full Text]  
• Kursar, M., Hopken, U. E., Koch, M., Kohler, A., Lipp, M., Kaufmann, S. H.E., Mittrucker, H.-W. (2005). Differential requirements for the chemokine receptor CCR7 in T cell activation during Listeria monocytogenes infection. JEM 201: 1447-1457 [Abstract] [Full Text]  
• Radstake, T R D J, van der Voort, R, ten Brummelhuis, M, de Waal Malefijt, M, Looman, M, Figdor, C G, van den Berg, W B, Barrera, P, Adema, G J (2005). Increased expression of CCL18, CCL19, and CCL17 by dendritic cells from patients with rheumatoid arthritis, and regulation by Fc gamma receptors. Ann Rheum Dis 64: 359-367 [Abstract] [Full Text]  
• Lee, B. O., Moyron-Quiroz, J., Rangel-Moreno, J., Kusser, K. L., Hartson, L., Sprague, F., Lund, F. E., Randall, T. D. (2003). CD40, but Not CD154, Expression on B Cells Is Necessary for Optimal Primary B Cell Responses. J. Immunol. 171: 5707-5717 [Abstract] [Full Text]  
• Lemos, M. P., Fan, L., Lo, D., Laufer, T. M. (2003). CD8{alpha}+ and CD11b+ Dendritic Cell-Restricted MHC Class II Controls Th1 CD4+ T Cell Immunity. J. Immunol. 171: 5077-5084 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search

TABLE OF CONTENTS