The Journal of Experimental Medicine
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Published online 9 June 2003 doi:10.1084/jem.20012065
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© Rockefeller University Press, 0022-1007/2003/6/1677 $5.00
The Journal of Experimental Medicine, Volume 197, Number 12, 1677-1687

Visualization of the Genesis and Fate of Isotype-switched B Cells during a Primary Immune Response

Kathryn A. Pape1, Valerie Kouskoff2, David Nemazee3, H. Lucy Tang4, Jason G. Cyster4, Lina E. Tze5, Keli L. Hippen5, Timothy W. Behrens5 and Marc K. Jenkins1

1 Department of Microbiology and Center for Immunology, University of Minnesota Medical School, Minneapolis, MN 55455
2 Division of Basic Sciences, Department of Pediatrics, National Jewish Medical and Research Center, Denver, CO 80206
3 Department of Immunology, The Scripps Research Institute, La Jolla, CA 92037
4 Department of Microbiology and Immunology, Division of Pediatric Immunology and Rheumatology, Howard Hughes Medical Institute, University of California, San Francisco, CA 94143
5 Department of Medicine and Center for Immunology, University of Minnesota Medical School, Minneapolis, MN 55455

Address correspondence to Kathryn A. Pape, University of Minnesota Medical School, Dept. of Microbiology and Center for Immunology, MMC334, 420 Delaware St. S.E., Minneapolis, MN 55455. Phone: 612-626-1188; Fax: 612-625-2199; E-mail: papex001{at}umn.edu

The life history of isotype-switched B cells is unclear, in part, because of an inability to detect rare antigen-specific B cells at early times during the immune response. To address this issue, a small population of B cells carrying targeted antibody transgenes capable of class switching was monitored in immunized mice. After contacting helper T cells, the first switched B cells appeared in follicles rather than in the red pulp, as was expected. Later, some of the switched B cells transiently occupied the red pulp and marginal zone, whereas others persisted in germinal centers (GCs). Antigen-experienced IgM B cells were rarely found in GCs, indicating that these cells switched rapidly after entering GCs or did not persist in this environment.

Key Words: lymphocyte activation • marginal zone • T cells • transgenic mice • germinal centers


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