The Journal of Experimental Medicine
for flow cytometry > invitrogen
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published 2 September 2002. doi:10.1084/jem.20020784
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 227K)
Right arrow PPT slides of all figures
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Miller, J. P.
Right arrow Articles by Allman, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Miller, J. P.
Right arrow Articles by Allman, D.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?
© Rockefeller University Press, 0022-1007/2002/9/705/ $5.00
The Journal of Experimental Medicine, Volume 196, Number 5, September 2, 2002 705-711

Brief Definitive Report

The Earliest Step in B Lineage Differentiation from Common Lymphoid Progenitors Is Critically Dependent upon Interleukin 7

Juli P. Miller1, David Izon1, William DeMuth2, Rachel Gerstein3, Avinash Bhandoola1 and David Allman1,2

1 Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA 19104
2 Abramson Family Cancer Research Institute, University of Pennsylvania School of Medicine, Philadelphia, PA 19104
3 Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01655

Address correspondence to David Allman, University of Pennsylvania School of Medicine, Biomedical Research Building 2,3, 421 Curie Blvd., Room 553, Philadelphia, PA 19104. Phone: 215-746-5547; Fax: 215-573-8590. E-mail: dallman{at}mail.med.upenn.edu

Little is known about the signals that promote early B lineage differentiation from common lymphoid progenitors (CLPs). Using a stromal-free culture system, we show that interleukin (IL)-7 is sufficient to promote the in vitro differentiation of CLPs into B220+ CD19+ B lineage progenitors. Consistent with current models of early B cell development, surface expression of B220 was initiated before CD19 and was accompanied by the loss of T lineage potential. To address whether IL-7 receptor (R) activity is essential for early B lineage development in vivo, we examined the frequencies of CLPs and downstream pre–pro- and pro-B cells in adult mice lacking either the {alpha} chain or the common gamma chain ({gamma}c) of the IL-7R. The data indicate that although {gamma}c-/- mice have normal frequencies of CLPs, both {gamma}c-/- and IL-7R{alpha}-/- mice lack detectable numbers of all downstream early B lineage precursors, including pre–pro-B cells. These findings challenge previous notions regarding the point in B cell development affected by the loss of IL-7R signaling and suggest that IL-7 plays a key and requisite role during the earliest phases of B cell development.

Key Words: B lymphocytes • hematopoiesis • cellular differentiation • cytokine • development


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS